FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n01_vk5-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n01_vk5-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4376292
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT29450.040.783811
GCCCACA28050.033.7062071
GCGTGAA36700.032.5195168
CTGCTTG76800.031.53555566
GCTTGAA91950.031.4020468
TGCGTGA26800.031.34238867
CTGCGTG27750.030.14328266
CGTGAAA41950.029.61774869
TGCTTGA85250.029.47716567
GCCGTCT111550.029.14753258
TGCCGTC140550.028.43746857
AAAGATC49550.028.3325315
GAGATCG86150.028.1213056
CCGTCGT29650.027.8575559
CTTGAAA110750.027.55677669
CCGTCTT126900.027.55240459
ATGCCGT145050.027.24155456
TATGCCG162100.026.66487755
TGAAAAA109900.026.65528970
GCCACAC21900.026.5425681
Produced by FastQC (version 0.11.5)