FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n01_vk5-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n01_vk5-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6089608
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA77500.024.8833168
GCCCACA19500.024.7798631
CTGCTTG76750.023.66722166
GCCGTCT91000.022.8456458
TGCTTGA79600.022.8198467
TGCCGTC105250.020.94964857
CCGTCTT100100.020.94358459
ACACTCT29850.020.8799821
ATGCCGT111250.019.6939456
TATGCCG113550.019.35667655
GTATGCC121100.018.29438654
TCTGCTT107200.017.00987665
CGTATGC132100.016.77100853
GTCTTCT118750.016.62278461
CTCGTAT124350.015.98677351
GATCTCG108500.015.93512548
TCGTATG137250.015.81020952
ATCTCGT111100.015.65671349
TCTCGTA119000.015.4408350
CTTGAAA129550.015.42612969
Produced by FastQC (version 0.11.5)