FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n01_vk5-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n01_vk5-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4467317
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA21350.036.4066051
GCTTGAA216700.036.22681468
GCGTGAA54900.036.21060668
CTGCGTG40350.035.64987666
TGAAAAA169000.035.62062570
CTGCTTG191600.035.60214266
TGCTTGA197850.035.1320167
TGCGTGA39500.034.82212467
GCCGTCT249100.034.2827758
CCGTCTT262550.033.59296459
TGCCGTC296050.033.48019457
GAGATCG125300.033.190046
ATGCCGT304200.032.93987356
CTTGAAA245550.032.86844369
TCTGCTT214500.032.69867765
CGTGAAA62300.032.69600769
GAAGATC55700.032.6177945
AAGATCG198550.032.5641256
TATGCCG318800.032.5401655
AAAGATC67600.032.365015
Produced by FastQC (version 0.11.5)