FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n01_vk5-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n01_vk5-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4468255
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT31750.037.054781
GCCCACA24100.035.7411
GCTTGAA98550.030.6132768
TGCTTGA95300.029.74755167
CTGCTTG94650.029.65601766
GCGTGAA17800.025.36464568
GCCGTCT130700.025.30552358
CCGTCTT138100.024.32969359
CTTGAAA128950.023.85759469
TGCCGTC153950.023.50714757
TCTGCTT123950.023.06931165
TGCGTGA15200.022.79558267
ATGCCGT163550.022.21293356
GTCTTCT138200.022.10880761
GCCACAC22750.022.0091511
TTGAAAA145550.021.80992570
CTGCGTG16450.021.7016866
TATGCCG170900.021.58527855
TGAAAAA102700.021.4357870
GTATGCC179350.020.66586954
Produced by FastQC (version 0.11.5)