FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n01_vk5-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n01_vk5-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2033053
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA14700.032.4014661
GGGGGGA5100.025.39137368
CGCGAGA1352.3104803E-723.33953
GAGATCG19950.023.33956
GGGTGGA4950.023.33261368
TTGCGGG6500.023.1531357
GCCACAC13950.023.0970611
GGGGGAA5650.022.91964569
ATCGGGG22200.022.5443549
GCGTGGA2350.022.33973568
AATCGGG25450.022.0032548
GAATCGG27800.020.89863447
TGGCGGG6450.020.61951857
TCGCGGT6700.020.37250550
CGCGGTT5850.020.34125151
GCGGGGA2252.1282176E-1020.22159867
GGGGAAA7300.020.13636470
CCGGCGT2800.019.99938259
TCGGGGG11950.019.32994750
GGGTGGC4350.019.30974854
Produced by FastQC (version 0.11.5)