FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n01_vk5-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n01_vk5-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4493
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA150.002081698770.0000167
TGCCGTC200.00650785752.556
TTCTGCT200.00650785752.563
TGCTTGA200.00650785752.566
ATGCCGT200.00650785752.555
CTGCTTG200.00650785752.565
GCCGTCT200.00650785752.557
CTTGAAA200.00650785752.568
Produced by FastQC (version 0.11.5)