FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n01_vk5-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n01_vk5-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6513243
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAA39650.033.71929666
CTGCGTG32150.031.2434764
ACACTCT32200.030.5561641
TGCGTGA31300.030.41465265
CGTGAAA47400.029.60904167
GCCGTCT88600.028.95533456
GGCGTGA12300.028.17010365
GAGATCG91100.028.1670026
TGCCGTC113150.027.59107455
TATGCCG135700.027.1843653
GCCCACA20150.027.108031
ATGCCGT121200.026.56706254
CCGTCTT112350.025.91842557
CTGCTTG73300.025.73612464
GTATGCC151550.025.5883452
GCTTGAA92950.025.37866866
CGTATGC150100.025.3458751
AAGATCG161350.025.2328196
ATCTCGT130000.024.84944747
TCGTATG144750.024.68683850
Produced by FastQC (version 0.11.5)