FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n01_vk5-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n01_vk5-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5110603
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT29800.035.129211
GCGTGAA41950.032.871966
GCTTGAA204600.032.65583866
CTGCTTG185650.032.3883464
TGCTTGA191850.031.83421365
GCCGTGT12200.031.5568356
CGTGAAA46750.031.14384367
GCCCACA14350.030.7421
ACGAGTG5700.030.70122338
CTGCGTG33650.030.05891464
TGCGTGA32900.029.78671865
CTTGAAA233150.029.70782767
GCCGTCT246400.029.68698556
TCTGCTT209550.029.59624163
CCGTCTT257100.029.3907857
GAAAAAA320450.029.2708370
GGCGTGA11800.029.06729165
TGAAAAA314650.028.72030369
TGCCGTC288650.028.63972755
GAGATCG109950.028.5263066
Produced by FastQC (version 0.11.5)