FastQCFastQC Report
Fri 17 Jan 2020
H27VNAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VNAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33612477
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3346010.9954666536476916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA528750.027.14161368
GAGATCG381300.026.7262846
GCCCACA111750.025.9086041
GCCGTCT618400.025.56738358
ATGCCGT718700.025.23215356
ACACTCT161400.024.8713591
CTGCTTG518800.024.75659866
TGCCGTC740150.024.36152657
AAGATCG655100.024.1478146
TGCTTGA550450.023.61295167
TATGCCG799700.023.58867355
CTCGTAT770400.023.2277151
TCACAGT1133550.023.22492237
ATCTCGT807600.023.08818849
GTCACAG1144350.023.04871736
TCTCGTA774900.023.03229950
CTTGAAA660550.022.72601969
AATCTCG586350.022.69044548
CGTCTGA1549350.022.0252523
AGTTCCG483850.022.01901641
Produced by FastQC (version 0.11.5)