FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length75
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAGATATTATAATTTTTAATTTTTTTTTTATTATATTATAAATTTATATTATTTAAATATTAATATTAATAA11.2658227848101267No Hit
GTGCTCTGCCGATCTATATATTATTTTTTTTTTTTTTTAATTTTATGTTTTTTTTTTTATTTATTTTATTTAGAA11.2658227848101267No Hit
CTCGAGAAAGCCCATCTGGGCCAAGCGGAACCGCTACGCCGCATGCCAGGACGGCTCTGTCTAATAAGCTTAAGA11.2658227848101267No Hit
GAGCTCTTCGGATCTTTTTTTTTTTTTATTTTTTTTTATTATTTTTTTATTTAAGTTGTAATATATGATTATTTG11.2658227848101267No Hit
CTTCCGAGGTTTGTGTTTTTTTTTTTTGTTGTTGTGGATTTTTTGTTTTTTTGATTGATATTTGGTGTTGATTGT11.2658227848101267No Hit
TTTCTATTCGGAGCAGATATTTTTTTTTTATATATAATTTTATAGCTTAGAGTTAACAGCGGACGTTAACAGCGC11.2658227848101267No Hit
CGTTAGCATCGCGATACTATATCGGTAATCTGTCACGCTTATCTATGCAGTCAAGCTGAACACTCTGTGGGTCGT11.2658227848101267No Hit
CGGGAGAACTTCTCCTCCACCGGCAACTTCGGTTTCGGCATCCAGGAACCTGTCTCTTATACACATCTGACGCTG11.2658227848101267No Hit
TTGTAGAAGTGTTGGCTTACGAGAGTTTTTGTTTTTTTTTTTTTTGTTATTTATGATTTTTTTGTTGTTTTATTT11.2658227848101267No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC11.2658227848101267No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTATTATTTAAATTTTTTTTTTTTTTTATTTTAATATTAT11.2658227848101267No Hit
TTTTTTGATTTTTTGTTTTTTTTTTATTATTATTTTTGTTATGTTTATTTTTTTTTTTTTTTTTATGTTTTTTTT11.2658227848101267No Hit
CTTCAACACTATTTTTTTATAATTTTTGAATTTTTTAAGAAATAAGACACTTAAACAAATCTGAAGCTGCCTAAG11.2658227848101267No Hit
TGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAGGAAATACACGTTGCTACTTTCATTGTAGCGCGCGC11.2658227848101267No Hit
GTACGGGGGGGTGGGGGGGGGGGGGGGTTGAGGTTGGTAGGGGAGTGGTGTGGGGGAGGGGAGGGGGGAGGGGGG11.2658227848101267No Hit
GGATAATGAAATAAGACCTCTGTGCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGACTGTCTCT11.2658227848101267No Hit
GTTCACTTCAGATCGGATTGATTTATAAATAAAATTTAGGAAATTAATGCTTAAGGGAGAAACCAACACATCGTC11.2658227848101267No Hit
GTACTATAACTTTGAACAAATTAGACAGATTAAAGCAGGCTACAAATGCCTCAAAATTCTGTGCATGGGATAATG11.2658227848101267No Hit
GTCCGCTTCCGATCTTTTTTTTTAATTTATTTTTTATATTATAAATAAATTAATATAAATTATAAGAATTATTAT11.2658227848101267No Hit
AACAAACAAGAAGGCAGAACTGCCAAAACCAACAACAAAAAAAAAACAAAACACAAAAATAACAAAAAAAAAGTA11.2658227848101267No Hit
GTGCTATTCGGATCTTTTTTTTTTTTTTTTTTTTTTTTTAATTTTTAAAAGAATTGAGAAGGCAGATATCTGGGC11.2658227848101267No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAATCCTTTAACAAGGACCAAATTGGAG11.2658227848101267No Hit
TTAATTTATATTATTTTTTTTTTAATTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTATATAATTTTTTTTATAT11.2658227848101267No Hit
CTAATGAACAAAAAAAGATAAAATCAAAATTATGAGTTTTCAAATATAATTACTCAGTAAAAATAAATAAAAATA11.2658227848101267No Hit
GAATAATACAAGCAGTGTGGGAACGTTAGAATTACGAAGTTCTGATTTAAAAGTATTACGCTGCAGCCGATATGG11.2658227848101267No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTGTTTTTATTATTTTTTTTTTTGAACTGTTAATACAAAGGGGGAGGGCC11.2658227848101267No Hit
TGTTGCGACGGTCCAAGATGTTCACATCTCGCGGGGTAATAGCGGGATCTGACTCTGAGGGGGCGCTGCAGGGGG11.2658227848101267No Hit
TTGCTAATCAAATCTTTTTTATTTTTTTTTTATTATTTTTTTTTTTTATTTTTTGTTTTATTTATTTTTTATTAT11.2658227848101267No Hit
TTACTAGACATAAAATAATTTTTATTTTAAATATTTTAGTTAAAGTCTGGTGCCACCCGCCGCGGTAATTAAATA11.2658227848101267No Hit
ACCCACTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCATATCCGAGGCCCTGTAATTGGAA11.2658227848101267No Hit
GTATACTTTAGTGTAGTGCAGTTTATTTCGTTTTAGCTTTATCTTTGCTTTGGGCAATTATTATCTTCATTCGCA11.2658227848101267No Hit
CTGATATAATGAGCCTTTTGCGGTTTCACTTTTAATTCGTGTGTACTTAGACTGTCTCTTATACACATCTGACGC11.2658227848101267No Hit
GTGCTATACCGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTT11.2658227848101267No Hit
TTTTTTTTTATATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTATATTATAATTTTATATTTATTTTT11.2658227848101267No Hit
CTAGGGCGGAATCTGATCGCCTTATAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTT11.2658227848101267No Hit
ATTTTAAGATGTATTTTGTTATTTTTTATTTTTTTGTTTATTTTATATTTTTATATTTTGTGTATTAGTTATTAT11.2658227848101267No Hit
TACCAGAGATCCTTACAGCAATTAATACTAACATCATATTAGAATATGTCTATTATTATCATATAACCACGCGAA11.2658227848101267No Hit
GGAAAAATTATAGATAAAAACATACAAAAAACAGAAGTTAAAATAAGCCCCAAAACACATCAGATTATTCTTTGG11.2658227848101267No Hit
GTGCTATGCCGATCGGTTTTTTTTTTTGTTTTTTTTTTTTTTTTATTTTATGTAATTATTTAGTTTTATATTGAT11.2658227848101267No Hit
ATATTTGATTTTTATTTTTTGAAAAAATATTTATTAGTTATTGATATTTATATGTGTAATAGAGAAGAGTCTTAT11.2658227848101267No Hit
CTTTTTTGTGTTTTTTTTGTTGTTTTTTTTGATTTGTTGGTGGTTTTTTTTATGTGTGTGTTTTAAAGTTGTGTG11.2658227848101267No Hit
GTTAATGACTCACACTTACTGGGATTTCCAAGTTCATGTGAACAGATTCAGTTCACAATCCCAAGCATGAAAGTG11.2658227848101267No Hit
ACATGCAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTA11.2658227848101267No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAATCCACCCACACAAAAAAAAATAAAAATTATAA11.2658227848101267No Hit
GTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTATTTTTCGTCAC11.2658227848101267No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG11.2658227848101267No Hit
CGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGCGGGGAGGGGGGGGTGGGGGGGGGGGCGGTG11.2658227848101267No Hit
AGATGTTTTCATTAATCAAGAACGAAAGATAGACGTTCGAAGGCGATCAGATACCGCCCTGACTCTTATACACAA11.2658227848101267No Hit
GTGCTCTTCGGATCTTTTTTTTTTTTTTTTTTTTTTTTATGTTATTTTATTTTGTATGTTGGGAAGTTATGCAGT11.2658227848101267No Hit
TTATTTAAATTTTTATTTTATTTTTAAAATAATAAAATTTTAAAATAAAAAAAAATAATATAATATAAAAAAAAT11.2658227848101267No Hit
TCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTATATCGTTAACA11.2658227848101267No Hit
CTTCAGATCTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATTTTTTTTTTTTTTTTTTTTTTT11.2658227848101267No Hit
CTTTTAAGTCGTGTGTACTTAGACATGCATGGCTTTAATCTTTGAGACCTGTCTCTTATACAGATCTGACGCTGC11.2658227848101267No Hit
CTTTAATACTTTAAACGCGTTTTCAGTTAATCCCTTCACTCCAATTAATTCCTGTCTCTTATACACATCTGACGC11.2658227848101267No Hit
GCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCC11.2658227848101267No Hit
AGACTCATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAAT11.2658227848101267No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTTTTTTTTTGTGAT11.2658227848101267No Hit
TCCGTTGTATGTAGTACATTTGCGAGGCAGATAACGCCTGTCTCTTATACACATCTGACGCTGCCGACGATATAG11.2658227848101267No Hit
GTAAAAGGATTTAAAGTGTACTAAGTACAATTTCAGAACCTCGGATACTATCAATTATACACATCTGAAGCTGCC11.2658227848101267No Hit
GTATCTAGGACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCTGTCTCTT11.2658227848101267No Hit
GTGCTATTCAGATCTTGGTGTTTTTTGATTTATTTTTTTTTATTATATTTTTTTTTTTATTATTTTATTAATTTT11.2658227848101267No Hit
CATTATATCAGTTATGGTTACTTAGATAGTTAACAGTTACTTGCATAACTTTGGTAATTCAAGAGCTAATAAATG11.2658227848101267No Hit
ATGTTATACCGAACAAATTTTTATTATATTTAGTATATAAAATAAAATAATATATTAATGTTATATAGATTTAAA11.2658227848101267No Hit
GTTTTGAAATAGCACAGCAGCCGCAACACTGTCCGATTTGCAGAAAAACAAATCAATGAGCGTTTCAATTAATGT11.2658227848101267No Hit
GTTCTATGGCGGGCTGGTTTTGTTTTATTTTTGTAATGTTTGGTGTTGTTGTTTGTTACGTTTATTTTTTATACA11.2658227848101267No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.2658227848101267No Hit
GGGTCCGCCTCGGCCGAGGACTCGTAGCTGACTACGACGTAAGCTTGGGAATACATGAAAGACGGGAGCAACTCC11.2658227848101267No Hit
CTACCATGGTTGCAACGGGAAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT11.2658227848101267No Hit
CAACAGATCTATTTTTTTTTTTTTTTTATTAAATAAATAAAATTTATATAATAAAAAAATAAAGCAGCCGACGAT11.2658227848101267No Hit
ATGTTAATTTTCGTCACTACCTCCAAGAACTGGGAGTGGGTAATTTACGCGACTGCTGCCTTCATTTGATGTGGT11.2658227848101267No Hit
GATGGATTGTGCGTGTCGTTTAATTGATTTCAAGGATTGCCAAATGGCAGAAGTAAATTGAAACTGTCGTCGTTG11.2658227848101267No Hit
GTCTTGGAGCTATCGTTATGTAGGATTTTTTATGGTTATTTGAGTTTAATTTTGTGTTTGTTTATTGATGTTAAT11.2658227848101267No Hit
GGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAATCCTTTTACAAGGACCAATTGGAGGGCAAG11.2658227848101267No Hit
GTTCAGAACATATATTTTAAATTTTTTTTATTTTATTTTTATATATAAAAAATAACAACATGCCTACAAAAACAA11.2658227848101267No Hit
GTGCTATACCGATCTATTTTTTTTGATTTTTTTATTTTTATTTTTTGATAGTTAATTAAGAATCGTTCTGTCACG11.2658227848101267No Hit
GTATTGGCCAGTTGCGAGTGGCCAGAGGACCGAATGCGATGATTCACCCGCCGCGCAGCAATGTAGAGACTCGCT11.2658227848101267No Hit
TTTTTTATTAATGTTATTTTATTTTTATATAAAAATTTATATTTTATTTTTTTTATTAATTTATTTTTTTTAATT11.2658227848101267No Hit
GTATAGTACTAACAACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTAAACTACTGTCTCTTATACACAT11.2658227848101267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACC50.069.04
TATACCG50.069.05
GTACTTT50.069.011
TTATTTT50.069.069
TTTTTTA50.069.065
TTTTTAT50.069.066
ACCGTAC50.069.08
TTTTATT50.069.067
ACTTTTT50.069.013
GCTATAC50.069.03
CCGTACT50.069.09
TACCGTA50.069.07
CGTACTT50.069.010
TGCTATA50.069.02
ATACCGT50.069.06
TTTATTT50.069.068
TACTTTT50.069.012
CTTTTTT50.069.014
GTGCTAT50.069.01
Produced by FastQC (version 0.11.2)