FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5215985
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG665741.2763456950125431No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75530.1448048642777922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA29450.038.095361
TTCGGAA33150.034.255322
CGTGTAG70450.024.67854369
TACGTGT143750.024.26125569
ATCTGTG22900.021.2391417
GATCTGG114700.020.7854566
TTACGTG169600.020.3600268
CGATCTA181850.019.9599695
CGATCTG473400.019.7951555
ATCTAGC26850.019.013887
ATCTGGC52150.018.3222487
CCGATCT1188800.018.1024284
GATCTTG54950.017.9573926
ATCTGGT26850.017.7291587
GATCTAG62150.017.6535036
ATCTGGG40950.017.436927
ACGTGTA101000.017.41882368
TTCCGAT1263600.017.1212352
TCCGATC1284700.016.9282953
ATCTATG13950.016.8146577
Produced by FastQC (version 0.11.2)