FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4765514
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG344770.7234686541682597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA19650.031.4432851
ATAGTGT81950.026.1829869
CGATCTG259950.022.8163265
ATCTGTG19550.022.7613377
AGTGTAG36550.022.55735269
TTCGGAA29000.022.3741822
CCGATCT674900.020.6336444
ATCTGGC34500.020.596957
GATCTGG89800.019.7100186
TTCCGAT710700.019.5561162
TCCGATC717100.019.4360713
GATAGTG108750.019.34987668
CGATCTA145800.018.837155
CACTATA7800.018.58431439
CTTCCGA755450.018.559731
TAGTGTA45750.018.47364668
CGATCTT117750.017.698475
ATCTGGG31200.017.2474487
CATACGT6050.017.1092174
GTCCTAA31550.017.0672151
Produced by FastQC (version 0.11.2)