FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4209302
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG336280.798897299362222No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA15700.029.0235161
TTCGGAA21350.023.2814142
GAGGTGT87600.022.87851369
ACACTAT10850.020.9938438
CGATCTG299400.020.9179885
GATCTGG88350.020.5425156
ATCTGTG25150.020.1606337
CACTATA10600.019.86102339
CGATCTA156400.019.6578525
GGTGTAG48950.019.52013669
GACACTA12900.019.25849737
CCGATCT811050.018.9376344
TTCCGAT842650.018.3475722
AGGTGTA54400.018.13522368
TCCGATC858700.018.0697523
AGAGGTG113450.017.60474868
CTTCCGA896450.017.4517731
ATCTGAG29650.017.3335047
ATCTGGC37450.017.2232287
ATCTATG10300.017.0788147
Produced by FastQC (version 0.11.2)