FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56
Sequences flagged as poor quality0
Sequence length75
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGTTACTTGTTCCCCGGTTAGTTTAGTGACGTAGCCAATTGTGGGACTTTCTTGCTATAATTTTTAAGAATA11.7857142857142856No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACA11.7857142857142856No Hit
GACGTGTGCTCTTCCGATCTCGTCAGGGAGACGTGTGCTCTTACGATCTCGTCAGGGAGACGTGTGCCTGTCTCT11.7857142857142856No Hit
ATCTTCAGCAGCGGCGCAGCAGCAGTAACATCAGCAGCAGCGGACGCGGCAGCAACACCACCACCAATTGCCGAT11.7857142857142856No Hit
ACCATACTTCCCCCGGAGCCCAAAAGCTTTGGTTTCCCGGGAAGCTGTCTCTTATACACATCTGACGCTGCCGAC11.7857142857142856No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATT11.7857142857142856No Hit
TCTTAGTAGCGACACAAAGACCTTCAGTTAATGTTATTACTCGACTTATTAAATCTAATATACAAACCCGAATTG11.7857142857142856No Hit
CAACAAGATGAAGACCAGCTACCGCATCAAGCAGGTCCTGCAGACGCTCTTCAAGAAGCAACAGAAGCAGCAGCA11.7857142857142856No Hit
ATTCTGTGCATGGGATAGTGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAA11.7857142857142856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.7857142857142856No Hit
GCGTGGTGCCGTATGTGACCGATCCAGACTTGGCGCCCATTGGCGTGGGGCGCATCGAGGTGCTGTCTCTTATAC11.7857142857142856No Hit
GTCTTACGACGGTCCAAGAATTTCACTTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA11.7857142857142856No Hit
CCCGAGAAACACTCTCTCAGTTCGGATCGTAGGCTGCAACTCGCCTACGTGAAGTCGGAATCGCTAGTAATCGCA11.7857142857142856No Hit
GACCTCCAATATCTCCTTCAAGTTATTATGCAGCTCCAAGCCGCAAATTTACCTAACCATAACAGTCCAGTCTGG11.7857142857142856No Hit
CTTGTACACACCTCCCGTCACACCATGAGAGTTGGCAACACCCGAAGCCGGCGGCCCAACCCGCAAGGGAGGGAG11.7857142857142856No Hit
CTTCCGATCTCAGCGGGGGTGTGCTCTCTGTCTCTTATACACATCTGACGCTGCCGACAAAGGGATAGTGTAGAT11.7857142857142856No Hit
TCCAACAGCTGGACAACCTTCCGCGATCCGTCCCCCCATCGCATCATACGACGGTGCAGGAATATTAACCTGCTT11.7857142857142856No Hit
CTTCTGTGCCGGGAGTTAACGCAATAAGTACCCCGCCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATT11.7857142857142856No Hit
AAAGTATATTTTATAAAATAAGAGAGTTGTTGACTTACGATGTATTTTGTATAATACAATTTTGATCTCTTCTGC11.7857142857142856No Hit
GTTCGTCGGATATGTCGCCCTTGCCGCAGTCGAACTCCCCGTCGCAGAGCCAATTGTTCGGGATGCAGTGGCGGC11.7857142857142856No Hit
GATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTGATCTCGAGAGCTTGCTCT11.7857142857142856No Hit
GCAGATAAATTAATAAAAAAAAAACATTAGGCGTTAAAGGAGTGAGGATGCATGGCCATGTTTGCTTGTTTGCCA11.7857142857142856No Hit
CCGTTTATCTGAAGAGCTGACGAGTACGCAGGCGTGGAGGTCAGTGAAAAATCCTAGGCAGAGATGCTCGGAGAA11.7857142857142856No Hit
ACCATAAACGATGCCAGCTAGCAATTGGGTGTAGCTACTTTTATGGCTCTCTCAGTCGCTTCCCGGGAAACCAAA11.7857142857142856No Hit
TTTTATGTTTTATATTTTTTTTTTATTTTTATTTTTTTTTTATTTTATATATTTTTTTTTTGTTAATATTTTATT11.7857142857142856No Hit
GGCGGGGGGGGGCGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGG11.7857142857142856No Hit
GAATAATGAACAAAACAAAATATTTTTTTTTTTTTATATTTTTAAAATTAAATCACGGTATAGTAAAAACAAATT11.7857142857142856No Hit
TAGTTAGCCGGTGCTTCTTTACCCATTACCGTCACTCACGCTTCGTCCCCGGCGAAAGCGGTTTACAACCCGAAG11.7857142857142856No Hit
GGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGA11.7857142857142856No Hit
GACCAAAACCTTTCGTTTCACGTGAAGCGAATGAGAGAACCATTAAAGTAGCTACACACAAATGCTAGCTGGCAT11.7857142857142856No Hit
TATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAATCCTTTAACAAGGACCAGTCTCATAGACACAACTGAC11.7857142857142856No Hit
GATAGAAACAGCAAACGTAACCCCAATTACATCTCAAGTAAAAACCTAACACAAAGAATCAAGTAACTAATAACA11.7857142857142856No Hit
CTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGT11.7857142857142856No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG11.7857142857142856No Hit
GTGCTAATACGATCTTTTTTTTTTTTTTTTTTTTTTATTTTATATATTAATTTTTTTTTATTTTATTAAAATGTA11.7857142857142856No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTAATTTATTTTAATTAATTATTATTTAA11.7857142857142856No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTCTGTCTCTTATACACATCTGACGCTGCCGA11.7857142857142856No Hit
GTCGTAAGACTAACTTAAGCGAAAGCATTTGCAAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGA11.7857142857142856No Hit
GTGCTCTTCAGCTCTTTGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATTTAAGGAGGAAAATAAAA11.7857142857142856No Hit
GTACAGGAACACTAGCGGTCGATTACGAGTTGTTAGCCTTGGAGGATGGTCCCCCCATGTTCAGACAGGATTTCT11.7857142857142856No Hit
CTTCCTCTCTGAGTTTGTGGTTTTTTTTTTTTAAAAAGAGAAGGAGACGGGGAAGGAAAAACTGGGCGCTGTCGC11.7857142857142856No Hit
ACCAGGGGGTGGCGACTGTTTACTAAAAACACAGGGCTCTGCGAAGTCGTAAGACGACGTATAGTCTCTCTCTTA11.7857142857142856No Hit
GGTACTACGTTCGAGCTGCCTCGATCGCCCGACACATCTACCTCAGGAAGCACGTCGGTGTCGGCTGTCTCTTAT11.7857142857142856No Hit
ATTGTAAAGAAATTCTGTTCCCAAAACCAGGGTTGAAACAAGATAAGATCTCTAAAAGGAGCTTAATATCAACGT11.7857142857142856No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTATCATT11.7857142857142856No Hit
GGTCAGTGAAGAAGAATTGGCAGTGATGATGGGTGAAGAGGAATATAGTGGAGATATTGGTGGTGGGAGGAAATA11.7857142857142856No Hit
CATCAAACAAAACAAAAATTAAAAATACATCAAGACCAAAGAAAACTGACCAACCAACTCCAAATACAATTAAAA11.7857142857142856No Hit
CTTCCGAGCTGTTATTTTTTTTTTTTTTTTTTATTTTATTTGATAAAAAAGAAAGCCAATAATAAAGAACGGAAT11.7857142857142856No Hit
ATCATAAAGGGCATGATGATATGACGTCATCCCCACCTTCCTCCGGTTTATCACAGGCAGTATCGTTAGAGTCCC11.7857142857142856No Hit
CTTACATACATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACG11.7857142857142856No Hit
AAAAAGATCTTTTTGTTTTTTTATAATAATATGACGCGGAAACCACCGGTGTGGTTTGTTACGTGAGCTGTCTCG11.7857142857142856No Hit
TATTGATGAACCTGCCAGTATTTATAAGATAGTCTCAAAGATTAAACAATTCATGACTACTGTCTCGTAAACACA11.7857142857142856No Hit
GATTGAAGCAACCTAACCGATCGCCAGTCAGCGAATCGGCGCCACACTGCAGCACCTGTCTCTTATACACATCTG11.7857142857142856No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA11.7857142857142856No Hit
CATCTAATGCCTAGTTTCACGTTCGACCTGTTGGGAGGGAAAGCCAAACGCCGTTTTGAGATCATCTGCGAACCC11.7857142857142856No Hit
CCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTAGACCTGTCTCTTATACACATCTGACGC11.7857142857142856No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAATT50.069.069
ACAAAAT50.069.015
ACAAAAC50.069.010
AACAAAT50.069.068
TATATTT50.069.035
TAAATCA50.069.049
AAATCAC50.069.050
AAATTAA50.069.045
TAAAATT50.069.043
AATCACG50.069.051
TAAAAAC50.069.064
AAATATT50.069.018
TTTTTTA50.069.030
TTTTTAT50.069.031
TTTTTAA50.069.039
AATTAAA50.069.046
ATGAACA50.069.06
TAATGAA50.069.04
TTATATT50.069.034
TTTTATA50.069.032
Produced by FastQC (version 0.11.2)