FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4082925
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG163760.40108500645982964No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG161270.39498643741925216No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAA152010.37230661841694374No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA144650.35428032599178283No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA138650.3395849789060539No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTT135860.3327516425111899No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCAGTTT117730.2883472020671455No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108690.2662062124579805No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTTCATTATCCCATGCACAG108410.2655204295939798No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATT104290.25542962459511254No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATG100290.24563272653795992No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTTTCGCTTAA98080.2402199403613831No Hit
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTC86370.21153952129906867No Hit
TTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTT79950.19581549991733865No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAAC74540.18256519529503973No Hit
TTTCATATGTTCCTCCTATTTAAAAACCTGCATTAGTGCTCTTAAACGAGTGTTATTGTGGGCCGGTACTATTAC69640.1705639951750277No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC63750.15613806278587042No Hit
GTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCT60300.14768823821157626No Hit
GTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGA59330.14531249043271674No Hit
GCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTACCGGCCCACAATAACACTCGTTTAAGAGCACTAATGCAGG57710.14134474671956992No Hit
CGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACAT57270.14026708793328313No Hit
CCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTTTCGCTTAAGTTAGTCTTACGACGGTCC54910.13448691807956303No Hit
GGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGC54820.1342664878732771No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC52650.1289516706772718No Hit
CCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTTCAT51990.12733518249784162No Hit
GTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCA50270.12312251633326597No Hit
ATTTAAAAACCTGCATTAGTGCTCTTAAACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGT49190.12047735385783476No Hit
GTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCAT49090.12023243140640595No Hit
CTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAA48670.11920375711040491No Hit
TCTTATATGTGATTAAATACTTGTATCTTTTCATATGTTCCTCCTATTTAAAAACCTGCATTAGTGCTCTTAAAC48410.11856695873669No Hit
GCATTAGTGCTCTTAAACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGG48340.11839551302068982No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA48330.11837102077554694No Hit
CCATAAAAGTAGCTACACCCAATTGCTAGCTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTC47820.11712191627325998No Hit
GTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAA47070.11528499788754387No Hit
ATCAGATACCGCCCTAGTTCTAACCATAAACGATGCCAGCTAGCAATTGGGTGTAGCTACTTTTATGGCTCTCTC46360.11354604848239926No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA46330.1134725717469706No Hit
ATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAA45670.11185608356754043No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT45400.11119479294868263No Hit
GTAATAGTACCGGCCCACAATAACACTCGTTTAAGAGCACTAATGCAGGTTTTTAAATAGGAGGAACATATGAAA45380.11114580845839686No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC44850.10984771946582414No Hit
ATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGC44590.10921092109210923No Hit
ATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTC44390.10872107618925157No Hit
CCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTTTCGCTTAAGTTAGTCTTAC42380.10379813491553237No Hit
AAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTC41570.10181426305895895No Hit
GAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGA41030.10049168182124336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT202150.030.3979664
CTTGTGT33700.030.19756969
TCCGATC209200.029.6417983
TTCCGAT210900.029.4882892
CTTCCGA216600.028.9222531
GATCTGG39050.026.0645356
ATCTGGG18300.025.6351437
CGGTCCA81850.025.49923710
GGTCCAA82000.025.4105211
CGATCTG111250.024.2524575
ACGGTCC87600.023.7861069
GATCTAG16000.023.7203856
GATCTAC19050.023.3638136
GACGGTC89400.023.2686028
TGTGTAG14400.022.99792369
GCGTCGT89150.022.9895332
CGACGGT90650.022.9454967
ACGACGG91050.022.9257976
CTCGCGT89800.022.81753329
TCGCGTC90450.022.6591130
Produced by FastQC (version 0.11.2)