FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94
Sequences flagged as poor quality0
Sequence length75
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAAGATCTATTTTTTTTTTTTTAATTTAATTTTAATTTGAACAAAATAGAGTGCTTAAAGCAGGCTTCAAATG11.0638297872340425No Hit
GTGTGGTAGCGGGGGGTTGGGGGGATGGGGAGGGGGGCGGGAGAGGGGAAGGGGGTGGGCCGGCGGAAGGGTAGG11.0638297872340425No Hit
GTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATGTGATTAAATACTTGTATTTCTGTCT11.0638297872340425No Hit
GTGCTGTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTATAAAAATTAAAATAAGAAGAT11.0638297872340425No Hit
CATAATATCTTTATATTATTTTTTTTATATATATTTATATTCCAGGGAAACCACAGCTTTTGGGATCAGGGTTAA11.0638297872340425No Hit
AAGTTATTTTCATGGAGCTAATGCATTAATTAATATGTGAAAATCTATGTGTCGACGTCATAGAAGACGAACTTA11.0638297872340425No Hit
GTGCTCGTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGATTTTTGGGGGAATTAGTATTAGGACG11.0638297872340425No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTATAAATTTTTTAAAAGTTGAATTAAATATTAAGAAACCTG11.0638297872340425No Hit
GTTCGGATCTTTTTTTTTTTTTTTTTTATTTATTTTTATATAATAAAAATTAAAAAAAGAAGAAGAGATCCACTG11.0638297872340425No Hit
ATTCCGATCTTGGTGGTTTTTTTATTAGTTGTTTTGTGCACGTAAACGACAGTGATTGTGGGCATTTACGATTAC11.0638297872340425No Hit
CTTCGGAGCTTTGTTTTTTTTTATTATAATGATTTATTTATTATGTATATTATAAAAATATGAATATTAAGAAGA11.0638297872340425No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGATGTACGGAATAAAAAAATGAGTCGGCGAGA11.0638297872340425No Hit
GTTCTCTTCCGATCTATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTATTTTTTTTTTTTTT11.0638297872340425No Hit
GTGCAGTTCAAATCTTTTTTTTTGATGTTTAAGTTGTAATTGTATATTAAGCATCGGGTTAGGGGGGGACAAAAA11.0638297872340425No Hit
CTCTTTTAATGCTAGAATTCTAACCTAACCCCATGAATCTGGGGAAGGGACACTCTCGTTGGACAGTTTGACTGG11.0638297872340425No Hit
CTTAAGATCTTTTAAATTATTTTTTATTTATTTTTATATAGAAATTGCCAAACAACATTTCATTAATCAAGAAAA11.0638297872340425No Hit
GTTCTACGTGATGATCTTCATGCAGCAGGTGGCGAAGATCGATCTGCAGACCTCATACATTTTGATGGCAGTTGG11.0638297872340425No Hit
GATCATCCGATCTTTTTATATATTTTAATTTTTTTTATTTATAATAAAACAAAAAGAAAGAGCAAATATCTAAAG11.0638297872340425No Hit
CTTAAGATCTATTTTTTTTTTTTATTTTTTTTTTTTTTTTGATAAAAAGTATCTTGGGAATATTAAATACAGATA11.0638297872340425No Hit
ATACATAACATGCAGCACGTATGGTCTTGTACCGACGACAGCTCTTTCAAATGTATGCCCTATACACTTTTGATC11.0638297872340425No Hit
ATACGTTGCTCACACCTTTAATCCCAAAACATAAAAAGACAAAGACACACGAAATACAAAAGGTAAAAATTTATA11.0638297872340425No Hit
CTCTAGAGATGTTATTTTTTTATTTTTTTTTATTTTTTATTTTAATTTTTATTATTATTTTTATTTTATTTTTTT11.0638297872340425No Hit
ACCGATCTTTTTTTTTTTATTTTTTTTTTTTTAATTATTTGATTAATTAAAATATATTAGATATAAAATTTAGAT11.0638297872340425No Hit
GTGCTATACCGATATGTATTTAATATTATTAAATGTATATTATAATAATATTAAGATGAATAAGATAATAGTTGT11.0638297872340425No Hit
TAGCGATACCGATCTGTTTTTAAATGTTTTTATTTTTTTTTTATTTTTATATGTATTTTATTATTTTTTTTTTTT11.0638297872340425No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTATTTTTTTATTAAAAACACATAAAATAAAAAAAAAAACAGGGCAGGCTGA11.0638297872340425No Hit
TTGTTGGTTTTTTATAGTTTGTTTTTTTATATTTTAATTGTGTTTTATATTTATTTTTTTTAGTAATTGTTTTTT11.0638297872340425No Hit
GATCTTGTTTTTTTTTTTTGTTGTTTTTTTTTTTTTTATAGTATAAAATATTTATTTTTTATTAATGTTAGTTAT11.0638297872340425No Hit
GTATTTTTTTTTTTAATTTATTTTTATTATTTTAAATTAAAATATTTTTTTTTAAAAAATTTAATTTTAATATTT11.0638297872340425No Hit
GTCATATTCCACATCTGTTGCAGGCGTCGCTGTCACGCCGTTCGGTCCTTATTTGGCGCCGTATTATTGCTGTCT11.0638297872340425No Hit
ATTCCGATCTTTTTATTAGTTTTTGTTTTATATTTTATTTTGTAAAAGGGACTATTAATTGGAATTTGCTGGTAC11.0638297872340425No Hit
CTTCCGATCTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTATTTTTTATGATTTGAAAAATTCAGTAGTCAAG11.0638297872340425No Hit
CTCTTGCGGTCTTTTTTTTTTTTTTTATTATTTTTTTTTTTATACGAGCTGCATGGTTTCTAGATTTAAACCAAT11.0638297872340425No Hit
GTGCGCTTCGGATGTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAGAAATATTAAATGCTGTCTCTTATA11.0638297872340425No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT11.0638297872340425No Hit
TATTTTTAATTTTTTTTATTTTTTTTTTTTTTTTATTTTAATTTATTTTATTAATTAAATTGATTTAGTGGGTCA11.0638297872340425No Hit
CTTCATATATTATTATTTTTTTTTTATTTTTTATAATTAAAAGGACTCCCCACAAAGTTGCGTTGCAGATTACAA11.0638297872340425No Hit
CAACCGATGAATTAATATATAATATATTATAATTATTTTATTTATATTATATTATTATATTAATTTATTAAATAT11.0638297872340425No Hit
TGTTAGTATTTAGCCTTAGCGGAGGGGCCCCCTGTCTTCACAACGGATTCCTCGTGTCCGGTGCTACTTTCCTCC11.0638297872340425No Hit
GTGATCTTCAGATATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATT11.0638297872340425No Hit
CTTCAGATCTTTTTTTTTTTTTTTTTTTTTTATGTGATGTTATTAATTATATAGAACAATACGGGCACTAGCAGA11.0638297872340425No Hit
ATCTTTTTTTTTTATTTTTTTATTATTTTATATGTAGGGACCCGAAAGATGGTGAACTATTAAACAATAGGGCGA11.0638297872340425No Hit
TAACGAGAGTTCACTCGCTCACCTTAGGATGCTCTCCTCGACTACCTGTGTCGGTTTGCGGTACGGGCCTGTCTC11.0638297872340425No Hit
ATTAAAAAAATGAATCTAATCATAATTAAAACAAATTCAAAAACCTCAATTACTCCTCATAAAAGATTGAAAAAT11.0638297872340425No Hit
CTTCCGATCTTTTTTTGTATTTTAATATTATTTTTTTAATGTAACTAGGAACCGTAAACAAAACCGACACAGGTG11.0638297872340425No Hit
GTGCACTGGCGAGCTATATTTTTATTTTAATTTATATTATTTTTTTATATATGTTATATATGTAATTTTGTGGAA11.0638297872340425No Hit
GAAAATTAAGTAATTTTAATAATTATTTTATAAATATAATTTCTGTCTCTAAAACACAACTGACGCTGCCGAATA11.0638297872340425No Hit
CTTTTCATATGTTCCTCCTATTTAAAAACCTACATTAGTACAAATAAACGAGTGTTATTGTGGCAAGATACTATT11.0638297872340425No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTATTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0638297872340425No Hit
GTGCAGGGCTATGATGTTTAGATTGGTTATGAATGTGATTTATTTTTGAATTTTTCGTTAATTATTTTTTTGAGT11.0638297872340425No Hit
GTGCTAGTGCGGTATGTTTTATAGTTATTTTGTATTTATTTATTTATTGTTTATTTAATTGATATTTTTATTATT11.0638297872340425No Hit
GTGTGGGTGTGTGGGGGGGGTGGGGGGGGGGGGGGGGTGGGGGGGTGGGGGGGTGGGGGGGGGTGGTGTGGGGGG11.0638297872340425No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTATTGATTAATAGAAGCAGTTT11.0638297872340425No Hit
ATCCATCAGTTTGTCAATCAATCAACTAACTGGCACATGAAGTATGCAGAAACTTTTAACAAACAACTTGATCGA11.0638297872340425No Hit
ATTCAGATCTTTAATATTTATTTTTTTATTTTTATTTTAAAAATCACAAGCAAGAAAGTGGTTAAGAGTTATACA11.0638297872340425No Hit
CTTCAGATCGGAACATTGTATATTTATATTATTTTATTATAAAAAATATATATGTACAAATGGAAGCTCCATGCA11.0638297872340425No Hit
TTTTTTTTTTTTATTTATATTATATTTTTATTTTTTTTTTTAAATTTATTTTTTTTTTTATATTTATATATTTTT11.0638297872340425No Hit
TCTTACGATTTTGCAGAGCCCTGTGTTTTTGATAAACAGTCGCCTGGACTCTTTCACTGCGGCCAGCATTGCTGC11.0638297872340425No Hit
ATTCAAATCGTTGTTTGATATTTTAAAAAGGAAAGGGTATCCTTGCTGCTGATGCTGTCTCTTATAAACATCTGA11.0638297872340425No Hit
TCGTCGTCCGATCTTGTTTTTATATTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTGTATTTTTTTTTTAAAGAT11.0638297872340425No Hit
GTGCGGTTCGGCTGGGTTTTTGTTTGTGTTTTTTTTTTTTTTGTGTTTTTGTGTTTTGTTTGTGGGTTGGATTGT11.0638297872340425No Hit
GTTTGTTTGTTTTTTTTTATTTTTATAGGTTAGTTTTTGTTTGTGGGATTTTAATGGGGGTAGGTTGTTTTTTGT11.0638297872340425No Hit
AAAATAAAAAATAACAAAAAACATAAAAAAAAAAACTAAAAAAAAATAACTAAAAAAAAACAAAAAAAAAAAAAA11.0638297872340425No Hit
ATGCAGATCCGAACCTCAGAGCGCTTCCCGGAAATCCAATACCTGGAAGAAGTGGATCCCAGTGGAGTCAGTCTC11.0638297872340425No Hit
CGTCGGATCATTTGTGATGTTTATTATTTTTTGTTTATTGGTGATGGCATAGCCCGTGAGGGTAAGCACGGACTT11.0638297872340425No Hit
TGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAGGAAA11.0638297872340425No Hit
AGCTTTGGTTTCCCGGGAAGCGACTGAGAGAGCCATAAAAGTAGCTACACCCAATTGCTAGCTGGCATCGGTTAT11.0638297872340425No Hit
TACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGCTGTCTCTTATACACATCTGACGCTGCCGACGCTGC11.0638297872340425No Hit
GTGCTCTTCCGATCTTTTAATATTTTTTTTTTATATTTTTTTATATAAAATTTTTAAATTTTAAAAGATTTAAAG11.0638297872340425No Hit
TTGCTAGTCCGATATTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTATATTTTTTTTATTTTT11.0638297872340425No Hit
GCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCACGCCGCGTG11.0638297872340425No Hit
GACTTGTGGATCGGAGTGAAAGGATAATAAAGATATGAGATTGATATTTCTCATACTCTCTCTTATACACATCAG11.0638297872340425No Hit
CTTCCGATCTTTTTTTTAATTTTTTTTTTATATTAATAGAACAACAAAACCTATTAGTTTTACCAATAAACACAA11.0638297872340425No Hit
CTTCAAATCATATATTATTTTTTATTTTTTTTATTTTATAGAAGGGGCACAAACACTCTCTGGAGCCACTATACA11.0638297872340425No Hit
GTGCTCATCCGAACTTTATTTTTATTTTTTTTTTTTTTTTTTTTTATATTTTTATGATTAATATTTTTTTTTTTT11.0638297872340425No Hit
CAAAAACTAAAGAGAAAGAGTATCCCAAAGATATTTTCATTAATCAAGAACGAAAGTTATAGTTTCGAAGGAGAT11.0638297872340425No Hit
GTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCTGTCTCTTATACACATCTGACGCTGCCGACG11.0638297872340425No Hit
GAGCAGTTCCGATCTATATTTTTTTTAATTTTATAATTTAATTATTTTAAATTTTTTAGATTAATAAAAATAGAA11.0638297872340425No Hit
TTTTTATTTTTTTTATATTTTTTTTTTTTTTTATTTGTTTTATATTTTTAATATTTATAAATATTTTTTTTTTTT11.0638297872340425No Hit
CTTCGGAGGAGTTTTTTTTATGTTTAATTATATTAACGGGAAACCAAAGCAGAGGGGCTCAGGGGGAAGTATCGT11.0638297872340425No Hit
CTTCCGATCTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTGTTAGTTAGTTTTTTTTTTTT11.0638297872340425No Hit
ATTATTTTATATAAATTTTTCACTATTCCCATCCTGACTCTTATACACATATAACCCTGACGACGAACAGGATAT11.0638297872340425No Hit
GTACAGGTTAAATCGTTATTATTTTAGGTTTTTTTTTTATTTTATTTTTAAAGAATAGGAGGTATTTACAGCTCA11.0638297872340425No Hit
TTATAGAAGTGTGCTCTGCCGATCTTTTTATTTTTTATTTATTATTATATTTTATGAATCGCATTAGGGATGTAA11.0638297872340425No Hit
GATCATGGACCATCTTGAGTTGACAAGAACAGACATCGACTTCTTTGACCGCGAGGCGGTGTTCATTGACCAGCA11.0638297872340425No Hit
TGCCACCACAGCGCGTGGGGCAGGGCGTGCAGGGGATCGAATCGCTTGGACACGGGCTTTCTCCGGCGGATGCGT11.0638297872340425No Hit
ATGCGTAAATTTTTTATGATTATTTTTATATATTATAGATGTATTTTAATTAAATAATATTTTACAAATTAAATA11.0638297872340425No Hit
ACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAAGGCGATCAGAT11.0638297872340425No Hit
CTTCCGAGATATTTTGTTTTATGAATTTTTTTTATTTTTATATTAATTATTGATGGTTATAAGAAATGCAGGCCC11.0638297872340425No Hit
CTTCCGAACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGATAGGGGATAGAAGGTCCACATCGTTGATGGGTA11.0638297872340425No Hit
CACTACCTCCCCGTGTCGGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG11.0638297872340425No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTTCATTATCCCATGCACAG11.0638297872340425No Hit
GGTTATGATGTTGTTGTTTTTGGTTGTTGCCTGTTGTTGTTGTGTTTTTGATTTTTTTCAAGCCATTATAATTAT11.0638297872340425No Hit
GTGCTCTTCAGATCTATTTTTTATTTTTTTTTTTTTTTATTATTAAAACAAGATCTGAACTAGAGTAATTTAAAA11.0638297872340425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATT50.069.014
TAAATTT50.069.048
CAGTTCC50.069.04
TATATTT50.069.015
AAATTTT50.069.049
AATTTTT50.069.050
AATTTTA50.069.027
AATTTAA50.069.035
ATCTATA50.069.012
TTCCGAT50.069.07
GTTCCGA50.069.06
TATAATT50.069.032
TTATTTT50.069.042
TAAAAAT50.069.065
AATTATT50.069.040
AAATAGA50.069.068
TTTTTAG50.069.053
TTTTTAA50.069.022
TTTTATA50.069.029
ATAATTT50.069.033