FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4307154
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG141620.32880180276813875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG103800.027.158935
CCGATCT254250.026.464684
CGATCTA55300.026.3938795
TCCGATC264000.025.5018443
TTCCGAT264950.025.415422
CTTCCGA272700.024.8608381
ATAGTGT31600.024.5624969
CGATCTT44050.020.914785
GATCTGA31500.020.702566
ATCTGTG10150.020.3911657
GATCTAG25800.019.5256716
GATCTGG47650.019.116746
GACCGTT34000.018.6679127
ATCTGGC18500.018.0865967
CGATCTC90950.017.641615
GATCTGC49550.017.4088136
GGACCGT39500.017.2084566
CCGTTAT36200.016.8675429
GTCCTAA41100.016.629641
TTAGGAC40400.016.4851993
Produced by FastQC (version 0.11.2)