FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119
Sequences flagged as poor quality0
Sequence length75
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTATTCAGAACGTGTTTTTTATTTTTATTTATATTATTAAAATCTGACGATGCCGACGATACTCCTTGTGTA10.8403361344537815No Hit
CTTCTAATACAAACGATTTATTATTTTATTTATGTTTATAAAAACGAGGGACAACAGGCGCAGAATTGTGGGGGG10.8403361344537815No Hit
TATTATTTATAATTATTAATATTTTATTATTTTTTTAATTTAATATTTATATTTTTTATTTTTATTTAATATAAT10.8403361344537815No Hit
ATGCTCAGCCGATTTATTTTTTTAATATTTTTTTTTTTTTTATTTTGCGTTAAATGATCGCCTTCGAACATCTAA10.8403361344537815No Hit
GTGCTATGGCGATGGTTTTTTTTTTTTTTATTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT10.8403361344537815No Hit
GTCGAGACTGATTAGCGTAGATTCCGGCACCTTAACCTCACGATCCGATCATCCCGCATCGCCAGTTCTGCTTAC10.8403361344537815No Hit
CTCCCAAGGCTTCTTAAAGGAAATCATAGCCTGCCTACTCGTCGGGGCATCGTTTCTACACCGACGGCGAGGTAT10.8403361344537815No Hit
GGTGGGGGCTGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGCGGGTAGGGTG10.8403361344537815No Hit
GAGCTCTTCCGATCTAATTTTTTATTTTTTTTTTTTTTTTTTTATTATTTATTTTTTTTTTTTTTTTTATTTTTT10.8403361344537815No Hit
CGTCCGAGCTTCTGTTTTTTGATTTTTTTTATTTTTATATAGGAGGAACGATGAGGGTTTTTTTATGCTGTCTCT10.8403361344537815No Hit
CTTCCGATCTTAAAAATTATTTTTTTATAAATATTTTAAAAAAAAAAAAAAAAAAAAAAACGAAACAACAAAAAA10.8403361344537815No Hit
TTGTTAGTCGGATATACGCCGGGGGAATTGAGCTCGTGCGATGATTTAATAATTATATTTTTTTTTATTTTAGAA10.8403361344537815No Hit
CTTCCGATCTTTTTTTTATATTTTTTTTTTTTTTTTTTATTTTTTTTTAATAAAAATTATATTATTAATTTTATT10.8403361344537815No Hit
GGTAAATCCGCCGCGCAAGGCTGAGACCTGATGCCGAGCCGATTGTGGTGAAGTGGATGATCCTATGCTGTCGAG10.8403361344537815No Hit
GGATGCGGGGTGTGTCGCGGTAGCCCTTGCCGGCCGCGGTCGAGCGGGGCGGGGTGCGGGAGGCGATTCGGAGGG10.8403361344537815No Hit
GTGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGTGG10.8403361344537815No Hit
GTGCAATGTTGGCGCCGCCGGCTCTCTACTGTCGCTTCCTCCTCTCCTCCAGCGCCTGCTCGTTCTCCACCCAAT10.8403361344537815No Hit
CACTTCTAGGGATGTTTATTAAGTGTTTTTGTTTTGATGTACGGTGGAAGCCCGGGGGTGGCAGACGCTGGACCT10.8403361344537815No Hit
ATTCTACACCCTCCATGTCTTTTCACAATGTCAAACTAGAGTCAAGCACAACAGGGTCTTCTTTCCCCGGAGAAT10.8403361344537815No Hit
TTTTTTTTTTTTTTTATTTATTATTTATATTTATTAATTTTTTTTATAATTATTATATTTGTGATAAAGTATAAA10.8403361344537815No Hit
GTGCTCTTCCGAAGTATTTTTTATTTTATTTTTTATTTTTATAGCGCGTGAACACACAAAGTTATTTCTGCCAAT10.8403361344537815No Hit
GTATTTTTTAGTGTATATTTTTTGTTAGAAAATTATTTTTTTTTTAAATATATTATTTTTTTAAAAGGTTGGATT10.8403361344537815No Hit
CAGTTGGGTCTTCGTGTGGTATTTGTTTATTTTTTTTTTTTATTTTTTATTTAACTGTCTCTAAGACACATCTGA10.8403361344537815No Hit
GGTGAACGCGGACATGCTGAAGGAGCGGATCGAGGCGCAGGTGTTTCAGCCTCGGCCGCCTCCTGTCTCTTATAC10.8403361344537815No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.8403361344537815No Hit
CACCCAAATACTTGCATAGATGTTAGACTCCTTGGTCCGTGTTACAAGACGGGTCGATAAAAACCATTTCGTACA10.8403361344537815No Hit
GTTATCATCAGATGAATTTTTTTTTTATTTTAATTTTTTTTTTATATTTTTTATAATTATTATTTTATTTTATTT10.8403361344537815No Hit
GTGCTATTCAGATCTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTAATAGGTTTTAAGATAAAGAAGTAATGAT10.8403361344537815No Hit
TTATATAACTTTATTTTTTTTTATATAAAAAAAGACAGAGAGACCCTGTCGAAGAAAGTCCAATGGTTACTGCTT10.8403361344537815No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTATTTTTTATTTTATTTATGTTTATTAAATAATTGGTG10.8403361344537815No Hit
GCTCATACGATCTTTTTTTTTTTTATATTTTTTTTTTTTTTTTTTTAAGATAATGAAAAAGAAAAATTACAAACA10.8403361344537815No Hit
AATAACACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8403361344537815No Hit
GAACCGGCAGAATATTGCACTACGCATCTCAACCTTACTAGGAAAACGAATGCATAGAGAGTCAGAATCCCTTAT10.8403361344537815No Hit
CAAATACAAACTTTATTATTTTTATATTTTATAATTTATTATAAATAAATTAATATTTATAAAAAAAATAATTAG10.8403361344537815No Hit
ATCAACAGATCATTAAAAATTATAAATTAATATTTGGTAACCCGTTAAGATAAAAAGAGAGCCTAAAAAGAAGAT10.8403361344537815No Hit
ATGTTTAGTTGGTATAATTGTTTTTTTTTTTATTTTTATGTTTTATTTTTTGTTGTTTTGATAGTAAATTAATTA10.8403361344537815No Hit
CGGCCGGGGTGATTTGGTTTTGGAGTTTGATTTTGTATTTATATGGTTTTTGAATTTGATGGGATATAAAAAAAA10.8403361344537815No Hit
ACTAATCCGAAATAATCTTAAAAACATATCCTCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8403361344537815No Hit
GACATGTGCCTCACACATTGTATAAATGAATATATTTTATATATAGGTGGTAGAAGAAAAAAAAAAAAAAAAAAA10.8403361344537815No Hit
GCTCTAGATAACATGCAGATATTTTTGTGTTTTTAAGAAGACAGATCTTTCACATGTCTGCCCTATCAACTTTTG10.8403361344537815No Hit
CTTCCGATCAATTTTTTTTTTTTTTTATTTTTTTATTTTTTTTTTTTTTTTTAATATAATTTAATAAATTTTAAT10.8403361344537815No Hit
GTGCTCTTCCGATCTTTTTTTTATTTTTTTTTTTTTTTTTATTTTTTTTATTTTTTTTTTTTATTTTAGTAAGAG10.8403361344537815No Hit
CTCAACCGATCTTTATTTTTTATTATTATTTATTTATTGAAGACACCAATTTAGTTAAGAAAAAGTTCCAGAGGC10.8403361344537815No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTATTTTTATTTAATTGAAGATAAATTAGAGTATATT10.8403361344537815No Hit
ATCTGTTTTTTTGTTTTGTGTTTTTGTTGATTTAAGAAGCTAGTGTAACTGTCTCATATACACATCTGACGCTGA10.8403361344537815No Hit
ATTAAGATCTTTTTTTTAAATTATTTTTTTTTTTTTTATTATATAAAGGAAAACGCAAAAGGTTTTAATGATCTA10.8403361344537815No Hit
GTTTTTTGTTTTTTGGGTTTTTTTTTTTGTTTTTTTTGTTTTTGATTGTGTTTTTTTGTTTGTTTGTTGTTTTTT10.8403361344537815No Hit
ACTGAATACTAATGCCCCCAACCGTCCCTATTAATCATTACGGCGATCCTAGAAACCAACAAAATAGAACCGCAC10.8403361344537815No Hit
AACTTAATCTTATTAGATAAATATTGGTGAGAAAACAGGCATAGATACAATGGTAGTAAACACCGTAAACGGTGG10.8403361344537815No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGATTTTGAAAAGAAGA10.8403361344537815No Hit
GTGCTCTTACGATATTTATTTTTTTTTTTTTTTTTTTTATTTAAATAATTTAAAAAAAAAAAAAAAGAGGGCAAA10.8403361344537815No Hit
GTTTTTTTTTTTTTTTTTGTTTTTTTGTTTGTTTTTTGTTTTTTTTTTAAATTTTGTTTTGTTTTATATTTAGTT10.8403361344537815No Hit
CATCAGATCTAGTTTTATTAAAGATATATTATATGAAAGTATCACCCAGTAATTCATAGACAGAGAGGTCCGGGT10.8403361344537815No Hit
GAGCTCAGCCGATATGTTTTTAGAGGTATTTTTTTGATATTTAGTTGATCAGGTTAAAGTAAGGGAAACCCTGAT10.8403361344537815No Hit
GCTAGGGAGACGTGTGCTCTTCCGATCTTATTAGGGCTGACTGTTATCAACCTCTCAAGGTTCGGAGGAATAGAC10.8403361344537815Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTGGGGCCGGGGCGGGGGGGGGGGGGGGTAGAGGGGGGGGGCGCGCGCGGGGAGGGGCTGGGGATGGGGCGGG10.8403361344537815No Hit
GTGCTCAACCGATATTATTATTTTTTTTATTTTTTTTTTATTTATTATTTTTTATATAATTAAATTATATATTTA10.8403361344537815No Hit
GTGCTATTCCGAAATTATATTATTTTTTTTTTTTTATTTTTTTTTTTTTTTTATTTATATTTTTTTTTATATTTT10.8403361344537815No Hit
CTCTTAAAGTGCATGGAAATGAAGAACAAAATCACACTCTCACCTGATTATACATAGAATTAAAAAAAACTCATA10.8403361344537815No Hit
GAGCTCAAACCATCGAGTTTGAATTTATATTTTTTTTTTAATTTTTTGAGATATGTATAAAATACACATCGGACG10.8403361344537815No Hit
GCTTCTGGAAACGCACCCACATGTCGCTCTGGATGTACTCGAGCATATGGCCAAGGTGGATCGAACCGTTGGCGT10.8403361344537815No Hit
AACCACAATTGTAAGTTGTACTACCCGTATCAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAACTATAA10.8403361344537815No Hit
CTAAAGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATAATTTTTAAAATTTTATTTTAAATTTT10.8403361344537815No Hit
CTTCACAGCGGGGCGTTTTTGTTTTTTTTATTTTTTTTATAATTATATATAAATAATAGTATATAAAATAAATTG10.8403361344537815No Hit
GGTGCGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.8403361344537815No Hit
CTTCGGATCCGCTCTTTTTGGTTAGAGATTATATAAATGTTAAGAGTATGAAGTTGGAGAGAGAGATGCGATGTT10.8403361344537815No Hit
GATCTATTCCGATCATTTTTTTTTTTATTTATTTTTTTATTTATTTGTTGAATAGAAAAACGTAGAAAATGAGGA10.8403361344537815No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTAAGATATGGATATGGAGACGTGTGCGCTGGCAG10.8403361344537815No Hit
GGTGGGGGGGGCGCGGGTCTCGCGTGGCGGGTCGGGTGAGCTGAGGTCGGGTATGCGCATGTGAGGCTGCGGCGG10.8403361344537815No Hit
CATTTAAGGAACTCGGCAAAATACCTCCGTAAGTTCGCGAGAAGGAGGCCCGGTTTTTTGCGCAAGCAGAGGCCG10.8403361344537815No Hit
CATCGGATGTTTATTGTTTATTTTTAATATGTTTTTAATTTTTTTTATATATTATTTAAAATAGAAGAATATGTA10.8403361344537815No Hit
CTACCGATCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATTTAATGTTGTTTTA10.8403361344537815No Hit
GGGGCGGGGGGGGCGGTGGGGGGGGGGGGGAGTCGGAGGGGGGCGGCAGAGGGGCTGGGGCGACGGCAGGGCGGC10.8403361344537815No Hit
GAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATAGATCCTCGTTAAAGGATTTAAAGTGTACTCATTCC10.8403361344537815No Hit
GTGATGTTCCGATATTTTTTATTTTTTTTTTTTTTTTTAAAGGGAAAATATTTAAGTCATTAGTGCTCTTTGGAT10.8403361344537815No Hit
CTTTTTGTTTTTATTTTGTTTTTTATTTTTTAAGAGCCGAGGAGTCTAAGGGTAAATCAAAAAAAGGCCCGCCGA10.8403361344537815No Hit
TCTAGAAGGCGAGTGAACGGTTAAGAGATGATACTTTTCCTTAGGGACACAAATCAATTTGCTGCATGGCAGTCT10.8403361344537815No Hit
GTGCTGATCAGATCTTAAATAATTTTTTTTATTTTTTATTAAAATTAATTTATTATTTTTTTTAAATAATTTATT10.8403361344537815No Hit
GGGTGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGAGGGGGGGGGGGGTGGGGGGGGGGGG10.8403361344537815No Hit
GTGCTATGCAGATCTTTTTTTTTTTTTTTTTTAATGATTTTTTTTTTGGCCATAGAGGGTGATAGAAACTTAAAC10.8403361344537815No Hit
GTGGGGTGGGGGTGTGGGGGGGGTGGTGGGGGGGTGGGGGGGTGTGGGGGGTGGGGGTGGGGGGGTGGGGGGGGG10.8403361344537815No Hit
ATTCAGATCTTTTATTTTTTTTTTTTTTTTTAATTTAAAGGAAAAACGCTACAAAAGGAAAGTTTTTACAAACTG10.8403361344537815No Hit
CAAAACATCTAAATTTTTTTATATTAATATAAAAATTATTAATTTTTATAAAAAAATAAAAAAAAAAAAAAAAAA10.8403361344537815No Hit
GCCTAGGGCGTGTGATCATCCGAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAA10.8403361344537815No Hit
GATCCATTGTTTAGTTTTGTTAGGTTTTTTTGTTTTTATATTAAGAAGGGGTTGCATTATATAAGTGTTCTTCCG10.8403361344537815No Hit
GTTAATGGGTCTTTTATGATGACTTTCCTTTCACTGTGAGTCTCTGGCAACAGTGCTCTATATGGGTACTCTGCT10.8403361344537815No Hit
ATCTTTTATTTAATTTTTTTATTTATTTTTTTTTTATTTTTTTTTTTAATTTATATATTAGTTAAGATAAAGAAG10.8403361344537815No Hit
GTTCGGATAATTTTAATTTATTTTTTTTATTTTTTATTTTTTATTTTTAATTATTTTTTTGTTGATTTTATTTTT10.8403361344537815No Hit
CAGATCAAGAGGTAATGATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACTGTCTCTTATACACATCTGAC10.8403361344537815No Hit
GCTAGGGAGACGTGTGCTCTTCCGATCTTATTAGGGATAACTCTTATAAACATATCAAAATAGCGACGAATATAC10.8403361344537815Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGTGGGGCGGCGGGGGTGGGGGTTGTGGGGGGGGGGGGGGGGGGGGGGCAGGGGGGGGGGCGCGGGGGGG10.8403361344537815No Hit
GGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGAGGGGAGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGG10.8403361344537815No Hit
TAGTTCACCATCTTTCGGGTCCCAACATATATGCTCTTACTCAAATCCATCACTAAAGATCCAGATCGGTCGAAG10.8403361344537815No Hit
GTTTTCATTAATCAAGAACGAAGGTTAGGGGATCAAAAACGATTAGATACCGTTGTAGTCTTAACCTGTCTCTTA10.8403361344537815No Hit
GGGTGGGGTGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGTGGGGGGGGGTGGTGGGG10.8403361344537815No Hit
GTGGGCAGGCGAGGGTTTTTTATAATAATTATTATGTTTTTTTAATTAATTTTTGTATAATTTATTTTAAAATTT10.8403361344537815No Hit
TATATAGTGATTTTTGGTTTTTTATTTTTTTTAATAAAAAATATTAATTTATATTTTAATTAATATGTATGTTAT10.8403361344537815No Hit
CTAAAGAGATTTTAAAATAATTTATAATATATATTATTAATACTAATGCCCCCAAAAAGATTATATTAATCATTA10.8403361344537815No Hit
ATTCCGATCTTTTTTTTTTTTTATTTTTTTTTTTTAATATACACAGAACCCAATTGCATGATAATATTAACAAGA10.8403361344537815No Hit
GCAGATCACCCACGTGTTACTGAGCGGTGCGGGACGAGTCCACCGTGCAAGCAGGGCGGGATGGGTCGGCGTGGG10.8403361344537815No Hit
CAAAAGAACTAATAACAAAATTAATAAGGTTTGTAAACTGCAGTCTCATTAAAGTGCCCAAACAAATGATGCAAA10.8403361344537815No Hit
GTGGGGGGGGGGATGGGTGGGGGGGAGAGGGGGTGGTGGGGGGGAGGGGGGGTGGGTTGGTGGGGGGGGGGGGGG10.8403361344537815No Hit
CCAGTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCTGCTAAGTTCCGACCCGCACG10.8403361344537815No Hit
CCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGACACTATACATCACCTTACGGTTTAGCAGAGACCTGTGTTTT10.8403361344537815No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCAGTTTATTGCAAC10.8403361344537815No Hit
GTTTTGTAGAATCTCCCAAGTTTTACATTTATATGTTTCTCTCTCTTATACACATCTGACGCTGCCGACGAAGAG10.8403361344537815No Hit
GATATAATCAGATATTTTTAATATTTTTTTTTATTTTTTTTTTTAAAAGAATGTCACAAAAAAACATATGACGCT10.8403361344537815No Hit
GACCTAATACTATAAATATTTTTTAATAATTAAATATTTATTTTAAAAAAAAAAAAAAATAAAAAAAAAAAAAAA10.8403361344537815No Hit
GGGCGGTTCGGATCTTTTTTTGTTGGTTTTTTTTTTGGTGATTTAGTGGTCTTATACTAGTGTTATTGTGGGCCG10.8403361344537815No Hit
TTTATATACTACCGATGTTTTTTTTTTTTTTTTTTTTTTATATTATACAAATATGAATAAGAAGACGATACTGCG10.8403361344537815No Hit
GGCCGAGATTGATCGCATGGTGAACGAGGCTGAGAAGTACGCCGACGAGGACGAAAAGCATCGCCAGCGCATAAC10.8403361344537815No Hit
AAAAATAGTATGTAACTGTATTATAATAATAATATTTTATAGAATAAAATGTTTAGATTTAATATAGTTTTAAAT10.8403361344537815No Hit
GATCTGGAGCGGGGAGATTGCATGATCACAAACATTTCAATTTCGCTGAGTCTCTGGAGGAGACAGTGTGGCCAT10.8403361344537815No Hit
GTGCTCTTCCGATCTTTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAATTATCAAAA10.8403361344537815No Hit
CTTCCGATATTTATTATTTTTTTATATTTTATTATAATTTATTTTTATAATTTTTAAATTTATAAAATTTTTTTT10.8403361344537815No Hit
GTGCGGTGCGGATGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGAGGGAGGGTTTGGAAGAGAGGGGGGG10.8403361344537815No Hit
AGGTAATGATTAATAGAAGAAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTTAAATTCTTGGACCGTCGTAA10.8403361344537815No Hit
CTTCCGATCTATTTATAATTTTTATTTTTTTTTATTGGAACGACCAAAGACTAACATAAGAGAAATAATAAGCAA10.8403361344537815No Hit
GTGCTCTTCCTATCTTTTTTTTTTTTTTTTTTTTTATTTTGTTTATGAAAGAAAAACATAACACAGAGATTGAGT10.8403361344537815No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers