FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCGGAGCTGGGCTCCGGGTTCGGACAACACCCCTTCCACAGCCACCATCACCACCATCAGATGCTGTCTCTTA17.6923076923076925No Hit
GATTTGAATATCTCGACCAGGGAAATTGCTAAATAATGGCTGCTGCACCCAAGGATATTGAGAAGCCCCATGTCG17.6923076923076925No Hit
GGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAG17.6923076923076925No Hit
CGGCATGCCCGCCGGTGCGAAGACGGGCGGGAGAGGGCGAGGAGGTCGCGGGCAAGGGGGAGCAGCTCGCTGTGG17.6923076923076925No Hit
TTTTTTTATGTTTTTTTTATATTTTGATTTTTTTTTTTTATTTTTTTTTTTGTTATTGTTAATAAGGGTGTTTAT17.6923076923076925No Hit
ACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGACTGTCTCTTATACACATCTGACGCTGCCGACGAAGGC17.6923076923076925No Hit
ATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGCTGTCTCTTAT17.6923076923076925No Hit
GGCCAAGCGTGTCCACAACATTGGCTTCAAGAAGCGCGCGCCCCGCGCCATCAAGGAGATCCGCAAGTTCGCCGC17.6923076923076925No Hit
GGTAGGGGAGACGTGTGCTCTTCCGATCTGGTAGGGGAGACGATGTCTCTTAAAATAGATTATATAATACTAGAT17.6923076923076925Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTGTTAAAGGATTTAAAGTGTACCTGTCTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTC17.6923076923076925No Hit
CTTCAAGACCTCTGCCATCAGCTTCAAGCTGGGCGTTGAGTTCGACGAGGACTGTCTCTTATACACATCTGACTG17.6923076923076925No Hit
CGTCTCCCCTACGCAGATCGGAAGAGCACACGTCTCCCCTACGCCTGTCTCTTATACACATCTGACGCTGCCGAC17.6923076923076925Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CATTCAAGGAACTGCGTGCCGCATTGTTCGCCATGTCCCCAGCCGTATTGCTCCCGTCCGTTGCCAATACTGCAG17.6923076923076925No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)