FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length75
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCGAACAAAAAAAATAATAAATTTTTATATAGAAAGAGATGAATAATAGAATAAACATCGGCAGATACCGAA12.5No Hit
GTCCCAGACAGAAAAATAGAACATAAACCTACCATAAACCTAAAAATTATAAAAGACAGCGCAAAACGATGAAGT12.5No Hit
GTGCTCTTGCGATCTTTATTATTTTTATTTTTATTTATATTATTTTAGTCTTACGACGGGCCAAGAATTTAAAAT12.5No Hit
GGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.5No Hit
GTGCTCTTCCGATCTGTTAATGTTTTTGATATTAATGTATATTTTAAACATATGACGCTGCCGACGATACTCCTT12.5No Hit
AATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAAC12.5No Hit
ACCGTAAGTTGGATAAGTGTTGTATTTATATAGATAATACCTGCAATTAAAACATGAAGCTTAAGGGACATGTGC12.5No Hit
CGGGGAGCGCTCTAATATATATAATTTATATAATTTATATAATATATATAATATATATAATATATATAAGATATG12.5No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA12.5No Hit
CATCAGATCTTTTTTTTTTTGTTTTTTATTTTTATTTTTATTGGAGAACTGTGGTAATTCTAGAGCTAATACATG12.5No Hit
TTATTATATTTTTTTATTTTAATTTTTTATTTTTTTAAATTTTTAATTTTTATTAAACAAAAATAAATCTGACGA12.5No Hit
TCCGTAGACCAGATGGGTGCAGTACTGCAGGGCGGGCTCCAGAACGGTTAGGATCAGCTTGGAGAGGCCCTCGCG12.5No Hit
ATATTCCGAGATTTTATTATTTTTTTTTTAAATATTTTTTGTTCAAAGTAATAGTACCGGCAAAAAAAAAAACTC12.5No Hit
CAAACTATAATATTTATATTTTTATTATTTTTTTTAATTTTATAATTTTATTTTATTTATATATTTATATTATAA12.5No Hit
CTTCCGATATATTTATAATATGTTTTATTTTATATTTAGATGCAGCCACCCCCTGTAGGTATTCACTGACCGCTG12.5No Hit
CGGCAGTTCTTGGTTTTTTGTTTTTTTTTTTTTTATTGTGGGGTATGTGATAGGCTTCGAACATATTAGTTTCGT12.5No Hit
TATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAAGG12.5No Hit
CACCTCTAGCGTCGTACTACTAATGCCCACACACTGCTTCTATTAATCCGTACCTCTTGATCTGAAAACCAATGA12.5No Hit
GAGCGGCTATGATTTGTGATTTGTTTTAAATGACATAAACTGTCTCTTATACACATCTGACGCTGGCGCCGATAC12.5No Hit
CTTCCGATCTTTTTTGTTTTTTTTTATATAGTTGTGGATTAATTTTTTGTTTTTTAAGTTTATAAGTATAGATCT12.5No Hit
GGGGGGGGGTGGGTGGGGGGGGGGTGGGGGGGGGGGCGGGGGGTAAGCGGAGGGGGGAGCGGGGGGGGGGGGCGG12.5No Hit
GTTCTATTTTTACCATATTTAAGACTCAGTACTTGTTAAATAATGGTGGGGTCACTATAACATCTCAACAGGCCG12.5No Hit
TTTCTATTCCGAGCTTTTTTGTTTTAAATTTTTTTTTTAAATAGGAGACGAAGTTCTATGTACGAAATCGTCTCT12.5No Hit
GGTCAGATATAGTAATTTATTTTTTTATTATTTTTGTTGTGTGTCTTATAAAAATCAGAAGATGCCGACGATACG12.5No Hit
GATATAATGAGCCTTTTGCGGTTTCACTTTTAATTCGTGTGTACTTAGACTGTCTCTTATACACATCTGACGCTG12.5No Hit
TCTTCAGGGAACGGCGGGGACCGGCCTTGAAGTCAACACGCACGGTGTTCTTGGCGGGGCGCTGGGCATACTTGC12.5No Hit
GATATAATGAGCCTTTTGCGGTTTCACTTTTAATTCGTGTGTACTTAGACATGCATGGCTTAATCTTTGAGACAA12.5No Hit
CTTCAGATCTTTTTTTTGTTTTTTTTGTTGTTATGTTATTATGAAAGCGATGAAAAGCAAAAGCGCATCACCGGC12.5No Hit
GATAAGATATATATTTTATTTTTTATTTTTTTTTAATATAAAGAACAAATAAAAAATAGAAGAAGTTTGATGTAA12.5No Hit
GGGCTGGTGCGCTCGGAGGGGATTGTTGTTGATAATTTAATTTTTTATTTTGTTGTATGTATTCTGTGTATTATT12.5No Hit
GCGCGGGGGGTGGGGGGGGTGGGGGGGGGGGGGGGGGGGGAGGAGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGG12.5No Hit
CTTTAGTTGACCTCGTATTAAGTATATATTAATTGAAATTTTAATATTTTATTTAATAGAAATATATTTAATTTT12.5No Hit
GTCCAAGAATTTCACCTCTCGCGTCGTAATCCTAATGCCCCCAAACTGCTTCTATTAATCCTTACCTCTTTATCT12.5No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAACTTTAATATACGCTATTGGAGCT12.5No Hit
TTATATTTATAAAAATAATTTTAATTTTTAATAAAATTATTAATAAAAATTTAAAATAATAAATTTTTAAAAGAA12.5No Hit
GCTCACGTCCGCGGGTTTTTTTGGGTTGTGGTGTGGTATAGCGCTAAAGATCGGAAGTGGTCAGGTGACCATGTG12.5No Hit
GTGATAGAGAGATGAGTATTGTTTTTGTTTATTATATTATTATAAATAATTTAAAATTTAGATTTATGTATGTTT12.5No Hit
AGTTAGAGGTTCGAAGGCGATCAGATACCGCCCTAGTTCTAACCATAAACGATGCCAGCTAGCAATTGGGTGTAG12.5No Hit
GAACATATGAAAAGATACAAGTATTTAATCACATATAAGAACTCCACAGGTAATACGACTGTCTCTTATACACAA12.5No Hit
TGTATAAAGATTGAGTGATATGATCGCCGTAGCAACTCTAACTTTAGTGATTTATTAATTAAACACGCTTTGGCC12.5No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers