FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97
Sequences flagged as poor quality0
Sequence length75
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACAAACGCTGCTGGATGAAGTGAGCACCACGACGCCACCGACGACGACAAAGACAACCACAACGACGACCACA11.0309278350515463No Hit
ATGCTCAACCGAACGTTGTTTTTTTTTAAAATATTAAAGTTTAATATACGCTAATGGAGCTGGAATTACCGCGGA11.0309278350515463No Hit
AATATAAAAAAAAAAAATATTAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0309278350515463No Hit
GTTGCGATCGATTTTTTGGTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGAGAAAAAAAAAAGCAAAGGGCGCAAG11.0309278350515463No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTATTTATATTTTTATTATTTTAATAATT11.0309278350515463No Hit
GTAGATCAAGTGTCCGGTGGTGGTCGACTTACCGGAATCTACGTGTCCGATCACGACAATGTTAATGTGAATCTT11.0309278350515463No Hit
ACAATAACCCATCCACAGAATAATCACGAATATCAATCCTTCTTAAACCACTCAAAATAGTTAAAAGTAATAGTA11.0309278350515463No Hit
ATTCCTAAAACGTTTTTCATATACTGTAATGATAAATATACAAAGGTCACCACATCCCGCTCAGGCTGGCTCTCT11.0309278350515463No Hit
GGGCCTGACGCCTGCCCGTTGCCTGAAGGTTAAGAGGAGGAGTGCAAGCTCTGAATTGAAGCCCAGGTAAACGGC11.0309278350515463No Hit
CCGTTTCCAGGCATGAATTTTTTTTGATGTAATTTTATTTTGTTTGGAAAATCGGATGAAAGCAATTACAGAATG11.0309278350515463No Hit
CTTCAGATCTTTTTTTTTTTATTTTTTAATTATTATAATAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAACT11.0309278350515463No Hit
ATTTACGCGCCTGCTGCATTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTC11.0309278350515463No Hit
ATGTAAAGCGGTCCGTCCATCTGCAGCTGGTTGTGCGAATCGAAGAACTCGTCGAGCAGCATCTGCCGCGTCTGC11.0309278350515463No Hit
CGACTACACTGACGCGACGTTCGCTGAATTTTTCAAGCGCCACAAGATCGATATCCACAGCCAGAGATCTGTCTC11.0309278350515463No Hit
AAGAAGGACAACGTCAAGAAGAACTCGTCCTTCCGAAGCATTTCCTATGGCAAGAAGGACAAACAGAAACCGGCC11.0309278350515463No Hit
GCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGA11.0309278350515463No Hit
TTTTTTTATTTATTTATTTTTTATTAATTTATTTATTTATTAATGAAAAAATATTTTTAAAATGATTTATATTAT11.0309278350515463No Hit
GTGCTGAACACATAATTTTATTATTAATTATTTTTTTTTTTTAATTTATTTTTTAAATATTTAATTTTTATATTT11.0309278350515463No Hit
TATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATTATAAATATTATAAGGAATGAAGCGGGAGAGTAATT11.0309278350515463No Hit
TTCCAGTAGTTATATTCTTGTCTCAAAGATTAATCCATCCATGTATAAGTACAAACGAATTAAAAGTGAAACCGA11.0309278350515463No Hit
GACCAATTCATAACATTTATAAATTTATTTTTTTTTTAAAGGGAAGCGACTGAGAGAGCCATAAAAGTAGCTACA11.0309278350515463No Hit
GAGCACACGTCTCCCAGCCTAGATCGGAAGCTGTCTCTTATACACATCTGACGCTGCCGACGATACTCCTTCTGT11.0309278350515463No Hit
CTTCCGATCTGATATATATTTTTTTATTTTATATTTTTTAATTTGAATTATATTTTTATTTTTATTTTATTTTTG11.0309278350515463No Hit
ATTTTTATTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTTTTTTTTATTTTTTATTTTTTAATT11.0309278350515463No Hit
GGTTTGGAGGGGGGGGGTGCCGGCGGCGGGAGGGGGCGAGTGTCTATGCGGCGGGGCGGTGTGTCGGAGTTGGGG11.0309278350515463No Hit
TAAAAAAACATCACTCGTATAAAAACAAATGTAAGAACAATGCTTATATTTAAAATACAATAACAATCACAAATA11.0309278350515463No Hit
CTTCGGAGATTTGTTTTGTTTTTTTATATTTTTTAGTTTTTTTTTTATTTTTTTTTATTTTTTAGATGGGGATGT11.0309278350515463No Hit
GAGCTGTTAAGATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATATTATTAAGAAATTAATTTATAAGTCTG11.0309278350515463No Hit
GTTCCGATCTGTTTTTTTTATTTATTATTTTTATATTATAAGAGAGAGTTAGGAAGGTTTAAAAACTCAACGGGG11.0309278350515463No Hit
TCCTCGAAATTGTTCACTTGTTTACAAAAAAGGTAGGTATGGGCTATTTAGGCCTTTAGTTGCACCTGTCTCTTA11.0309278350515463No Hit
CTTCAGATCTTTTTTATTATTTTTTTTTTTTTTTTTAAAACACAATAAGAATTTAACAACAAACAAGAAGGAAGC11.0309278350515463No Hit
GAGCTCTTCCGATCTATAATTATTTTTTTTTTTTAAATAAAACCCCCCCCAAGTAGAGTAGATAAACAGAATGTG11.0309278350515463No Hit
GAAAACATCTTTGGCAAATGCTTTCGCTTAAGTTAGTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAA11.0309278350515463No Hit
TGTTTATTTTGGGTACCAACCAAACAGTGATTGTAGAATTTAAAAAAAAAAGCTAAGAAAACATGGCTCGTACTA11.0309278350515463No Hit
CTAGCAAGCTAGCCCGGCACGAAAGAAGAATAAATATATATTATATATAAAATATAAAATAATTTAATTTTATTT11.0309278350515463No Hit
CGCCGTGGCCATGCTGCACATCGTCAACAGCGCGGCCATCCCGGTGAGCTTCTGGAAGTCCTATTCCGCCTACGC11.0309278350515463No Hit
GGTTAGTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATGCTGTCTCTTATACACATCTG11.0309278350515463No Hit
TTTTTTTTTATTTTAATTTTTTTTTTTTATTTTTATATTTTTATTATTTTATTTATAATAATTTAAAAATTATAT11.0309278350515463No Hit
ATCAAGAGGTAATTATTAATAGAAGCTGTTAAGGTGCATTAGAATTAATACTAGCTCGATTACACATCTGAACCG11.0309278350515463No Hit
GGATATAGGCGCTCCGCCGGGAGTGCGGTTAGGTAGGGAGAGGTATAGTAGTGGGGAGGGTTGTAGGGTGTGGTG11.0309278350515463No Hit
TTTTTATTGTTGTTTTGTGGTGTTTTTGTATTGTTTTTTTTTGGTGTTAGTATTACTTCTGTATATTATACACGT11.0309278350515463No Hit
CATTGTTATTCCGTATTTCTTTATTAGAAGCGTTTTTGATTGATACTTGTGTCAACAACCTTCGTTAAGCTTTAT11.0309278350515463No Hit
CCATAGATGTGCTAGCATTTTATAAGTCTACCAATCCGCCAACAACACGGAATTCCAAGTTTCCCCTACGACAGC11.0309278350515463No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGGTTAAGCTCGCCACTGAATATCTGTCTCTTATACACATCT11.0309278350515463No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATAATTAAAAGTAGTATTTTAAACAAATTGAAGATG11.0309278350515463No Hit
TGTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTATTTTTCGTCA11.0309278350515463No Hit
CGACCGATCTTTGTTTTTTTTATATTTTTTTGTATATAATGGATTTAATTAGAGAGTGGGAATAAAACACAAGCC11.0309278350515463No Hit
TACTTGGACCGTCGTAAGACAAATGCCCCCAACCATCTTCTAGTAATCCTTACCTACAGATAAGGAAACAAATAA11.0309278350515463No Hit
CCCCTAGCCTATTCAGTGCTCTACCCCAAGTCATCAGCCTGTCTCTTATACACATCTGACGCTGCCGACGATACT11.0309278350515463No Hit
TGGTTTTTGTTTTTTTATATTTTTGTTTTTGTTTTTTTTTTTTTTTTTTTGTATAAAGAAAAAAAGAGGGTAGAG11.0309278350515463No Hit
TCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAATTAAAACATGAACCT11.0309278350515463No Hit
AAACAAATGCATCGAGGTTTTTAGTTTTGGGTTGTTTTTTATGGTAGTTTTTTTTTCGACAGCACTTGCCATTTT11.0309278350515463No Hit
CGCCCGGGAGACGTGTGCTCTTCCGATCTGCCGTGGGCTAACTCTTCTAATCCTCTGACGCTGCGGAGGATACTG11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCGTTAGGATGATCCAGCACCAGCTTGGTGCTACGGTAGTTCAAAAAATTTCGCAGTGTCAGGTCGCCGTCGCTG11.0309278350515463No Hit
GGTTTTTTGTATTTTTTTTTTTTTTTTTTTTTTAATATATTTATAAATATATTTTTATTTTTTGTAATCTAGATA11.0309278350515463No Hit
TTCCGATCATTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAGATCGAGGAGGTTAATTAGACAATAAAGTTGCAGA11.0309278350515463No Hit
ACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATAACATGCAGA11.0309278350515463No Hit
CAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGAT11.0309278350515463No Hit
ATCTAATTGTATCCCAAGCACGCCAGACTATCTCCATCTTCAGCGCCAAGACTTATACCGAAGGTACGCCACGAA11.0309278350515463No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTTTTATTTTTTTTTTAATTTTATATTTATTAATTATTT11.0309278350515463No Hit
ACTTCTATGTAATGCGATGAGAGGACGGAGAAGGTTAAGTCAGCCTGGCGTTGGTTGTCCAGGTGGAAGGTTGTA11.0309278350515463No Hit
ATTAACTGAAGATCGTTGATGGATATGTTTATTATTTATAATAATACAGACCGCCAATCGTATACTCCTTGAACT11.0309278350515463No Hit
AATAAAAAACATCAATTAGTTCTCTAAAACTATAGTTATTCTAGTCCTGTACTATTACTTTGAACAAATTAGAGT11.0309278350515463No Hit
GCTGGGCGCCGATGCCGTAAGGACGAGCCGCCTTCAAGTCTCGCTCGGCACGAGCGTACTTGGCAGCGGACACCA11.0309278350515463No Hit
TAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAA11.0309278350515463No Hit
GAGCTATAGGCTAATTATATCAGTTTTGAAAAATTAGATAATTAACAGTTTCATTTTTATATGATATAATACTAC11.0309278350515463No Hit
GTGCTATTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTGTAAAATAAAAAGAATAAA11.0309278350515463No Hit
GTACACAAGAATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGATATCTTTCATTAGAACGATAACATTTAA11.0309278350515463No Hit
CAGCAAAAGGCTCATAATATCAGTTAAGGTTCTGTCTATTATACACATCTGACGCTGCAGAAGAAACTCATTGTG11.0309278350515463No Hit
GTGTTTTAAATATTTTTTTATTTATATTTTATTTTTTTTATATTTATATTATTATTATATTTATATTATTATTAT11.0309278350515463No Hit
ATTCAGATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATGATAAAAATATGAAAAAAAAAAAAAATAAAGA11.0309278350515463No Hit
TTCTTGTTGCGCGCATCCTTCTTCTTCTGCGCCTACCGTTTCTTCGCGGCGGATGACATTATTCCGGCTTGGCGT11.0309278350515463No Hit
GTATAGATATTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTATTTTTTTTTTTTTTTATTTTTTTTTTTTTTTT11.0309278350515463No Hit
GTCCTCTACTTATCGTTGCCATTGTCCTGCTCCACCGCCTCCTTGGGACTGTCTCTTATACACATCTGACGCTGC11.0309278350515463No Hit
GAGCTCATCAGAACTATTATTTTTTTATTATATTTTTTTTTTTTTTTTTTAATATAATAATTATAAAATTTATAT11.0309278350515463No Hit
GTGCTATACGGATAATATATTTTTTTTTTTTTTATTTATTTTTTATTAAATTTTTTTTATAGTATATTTTTATAA11.0309278350515463No Hit
GAGCTCTACCGAGGTTTTGGTTTTGTTTTTGTTTTTTTGTTTTATTTAGTCAAGAACGATTGTTTGAGGTTGTAT11.0309278350515463No Hit
TGCAGTTACGATATTTATTTTTATTTTTTTATTTTTATAAACTAATTATCGAAAGCGAAATGTTTTACAGACTGC11.0309278350515463No Hit
GAGATAAATTCGAGATATAGTATTAAGTTTTTTTTTTTTTATTAAACTTTAAATACTTTAACAAGGAAAAATTGG11.0309278350515463No Hit
ATCGAACCCTGATTCCCCGTTACCCGTTGCAACCATGGTAGTCCTAGATACTACCATCTAAAGTTGATAGGGCAG11.0309278350515463No Hit
ACTAGGGGGTGTCATAGCCTACCGAATCCTGATGAACTCCGAATGCCACGTAATATCTGTCTCTTATACACATCT11.0309278350515463No Hit
TTTTTATTTTGTTTTTTTTTTTTTGTGAATGATATCAACCCAACAACCAATATAACGATCTTGCGATCGCATGGG11.0309278350515463No Hit
GATCGGAAGAGCACACACAAAAAAAAAAAAAAAAAACGCAAGAAAAAAAATCAAATAAAAAAAAAAAAAAAACAA11.0309278350515463No Hit
ATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAAGGTTCATCTTTTAATTGCA11.0309278350515463No Hit
ACCCGTATGAAGCACAAGTTCAAATAAGAACGTTATACCCGCAACAACTTTAATATACGCTATTTGGAGCTGGAA11.0309278350515463No Hit
GATATAAAACGTTCCCAATTCAAAATCGCGTCACATAATAAACAAAAAGTTAAAGCATCTTAAAAGGACTGATAT11.0309278350515463No Hit
ATACACATCCATAATTATTTTTTTTTTAATAATTTTTAAAGATCTGTCGTCGGAACAAGACCATACGCACTGCAT11.0309278350515463No Hit
ATCAGATACCGCCCTAGATCTAAACATAAACGATCCAAGATAGAAATTGAGTGTCGCAACTAATATGGCTCTCTG11.0309278350515463No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGCTGTCTCTTAT11.0309278350515463No Hit
GCTCTAACGATATTATAAATTTTTTTTATTTTTTTTTTATATTTTATAATTTATTATATTTTTTTTTTTTTTTAT11.0309278350515463No Hit
GAACATAAGTGTGTAAGACAGATTGATAGCTCTTTCTCGAATCTATGGGTGGTGGTGCATGGCCTGTCTCTTATA11.0309278350515463No Hit
CTACCGATCTATATTTTTATATTGTTTTATTTGTGTATTTATAATAGAGTTAAGCAACATATAGGAGGCGCAGCT11.0309278350515463No Hit
TTTTTTTTATTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0309278350515463No Hit
CTTCCGATCTTTTATAATTTTTTTTTTTTTTTTAATTTAGGAACGGTGCAATTATAAGAACAAGAGATGCGAGCC11.0309278350515463No Hit
CTATTAACATTTATCTATGGATGTAATAGTTAATGATTCTATTTTATATATGTATCTTATAAAAATCTTACTATT11.0309278350515463No Hit
ATTCCGATCTCAACCGGGAGAAGTGTGATCTTCCGATCTTTTTTTTCTGTCTCTTATACACATCAGACGCTCCCG11.0309278350515463No Hit
GTATTTAATCACATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGTA11.0309278350515463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGG50.069.030
TCCTTAG50.069.01
CATGAAC50.069.067
CAGTTAC50.069.016
TCTAGAG50.069.041
TTACTTG50.069.019
CGTTAAC50.069.010
AATTCTA50.069.038
AAACATG50.069.064
ACATGCA50.069.053
TGCAATT50.069.056
AACTGTG50.069.029
ACATGAA50.069.066
ACTTGGA50.069.021
TGGTAAT50.069.034
TAAAACA50.069.062
AGCTAAT50.069.046
GATCGTT50.069.07
GATAACT50.069.026
AATTAAA50.069.059