FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131
Sequences flagged as poor quality0
Sequence length75
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96.870229007633588No Hit
GGGTGGGGGGGGGGGCGGGGGGGGGGGGGAGGGCGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.7633587786259541No Hit
CAGATAATAATAACTAAAAAAATTAATTAAATAATTAATTTTAAAGACTAATGACTCTCTCTTATACACATATTA10.7633587786259541No Hit
GTAGTTGGTTTTGTTTAAATTGATTTTGATAATTATAAGTATAGTGTGAAAATGTGATATATGAGGATCCGTAGT10.7633587786259541No Hit
CCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAA10.7633587786259541No Hit
ATGTGATCCAGTCTGGCCTGGAGAACCACGATTCCGGCGTGGGCATCTACTGTCTCTTATACACATCTGACGCTG10.7633587786259541No Hit
GTTATCATCAAAAATTTATTATTTTTTTTTATTTAAATTTTTATATAAAGAAACAAAAACAAATCTTAAAATTAA10.7633587786259541No Hit
CAGCTGAGATAGTTAATTGTATGTTTTAAATTGTTGGGTCAAATTAACCCGCAGGCTAAAATCCAGGTGGTGCCC10.7633587786259541No Hit
GTTTTTTTTTAATTATTTATTATAATTTTTAATTTTATATTAATTAATTAGATAATATGAGAAACCCTGTCTCTT10.7633587786259541No Hit
TTGTTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTTCATACGGGTAGTACAACTTCTGTCTCTTATACACATC10.7633587786259541No Hit
TGTCTGCCCTATCAAGTTTTGATGGGAGTATCAAAGACTTCCATGGTTGCAAAGGGTTAAGGGGATAAAGGGTTC10.7633587786259541No Hit
GGGTGGGGTTGGGGGGGGGCGCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGG10.7633587786259541No Hit
CTAAAGTGGGAGACGTGTGCTCTTCCTATCTCAAGTACAAAACGTGAGCTCAACAGTACTCAATAAGGATACATG10.7633587786259541Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTAAGC10.7633587786259541No Hit
TAACTTCCCATAATTAATTATTTATTTTTATATTTATTTTTAAATTTATCAAAAAAAAATATTAATAAAAATAAG10.7633587786259541No Hit
AGTTTGGGGTGGATTACCGATGATCGGGTGTCGTGGATAGTGTATGACATTGGGGTGGGTGGGGGTGTGAAGGCG10.7633587786259541No Hit
ACACGACACTCGTAGGCGCTGACACCAAATTTGTGGCCGCTGGTCTAGTTAATCTCTAACTGGACGTGGTGTTAC10.7633587786259541No Hit
GTCCTTTCCGGATCGGTACTTGGCGTGGCTGGCGCTCTAATCACCGCCCACTATATAGCTACTGTCTGTTATACA10.7633587786259541No Hit
CCCGTATGAAGCACAAGTTCAACTACGCACGTTTTAACCGCAACAACTTTAATATACGCCTGTCTCTTATACACA10.7633587786259541No Hit
GTTCAACTACGAACGTTTTAACCGCCACAACTTTAATATAAGATATTGGAGGTGGAATTACCGCGGCTGCTGGCC10.7633587786259541No Hit
CACCCATACTTCCCCCGGGGCCGAAAAGCTTTGGTTTCCCGGGAAGCGACTGTGAGAGCCATAAAAGTAGCGACC10.7633587786259541No Hit
CCCCAAAGCGCACTTTCAAAATGAATATATCATGTTGTTCCTAAAAGTATTCCGGTATGAAACACCCAGACTGGG10.7633587786259541No Hit
GATCAGATATATGAATTATTTTTTATTTTAATTTTAAATTTTATTTGTTTGATTAATATTTTAATAATTTAAGAA10.7633587786259541No Hit
CTTCCCAGCTTTTTTTTTTTTTTTTTTTTTTTTATGGAGTGGCACCATCAACCACGAAAACACGGCCGAGGTGGA10.7633587786259541No Hit
GTGTGTGGGGGGGTGGGAGGGGGGGGGGGGGGCGGGAGGGGAGGCGTGGGGGGGGGGGGGGGTGGGGGGAGGGGG10.7633587786259541No Hit
GATCGGAAGAGCCCCAGGCCAGATCGGAAGCTGTCTCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGT10.7633587786259541No Hit
TTATTTTTTTATTTATTTGTTTTTTTTTTTTTATTTTTATATTTTTTATATTAATTATATTTATAATTATAAAAT10.7633587786259541No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTATATTAAGTTATTCAAAAGAAGGACAAAGAAAT10.7633587786259541No Hit
GATCGGAAGAGCACACGTCTCCCTGTCTCTTATACACAACTGACGCTGCCGACGACTCCAAACGTGAAGATCACG10.7633587786259541No Hit
CCGTCCGGCTGCCCAGCGATTTCCTGCTGGCGCTGCCGGCGTCGGCGTCGCTGCCGCCGATACTCCGTGTGTTGC10.7633587786259541No Hit
CTATTAAGATCTGTTATTTTTTTTTTTTTTTATTTTATTTTTATAATGAAATAAGAAATATGTTATGGTTTCATT10.7633587786259541No Hit
GTGCTCTTCCGAACTTTTTTTTTTTGGTTTTTTGTTTTTTTTTTTTTAATATTGATAGTGTAGTTTTGTTGGTGT10.7633587786259541No Hit
GATATCAGCAGGAAGAAGAGTTTAGTTTGAGGTTATATGTTAATTTAATGAGTATGATGAGATATAGAGTGGGTA10.7633587786259541No Hit
TCCACTACCGAACATTTTTTATTATTTATAATTTTTTATTATTTTATTAATAATTTTTATTTTATGGATTATAAA10.7633587786259541No Hit
GGCGTGGTGCGGTTGCCCTGGTCATTGGCGATAATCTCAACCTTGCCATGCTGGTAGACACCCACGCAGGAGTAG10.7633587786259541No Hit
CCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCCTGTCTCTTATACACATCTGACGCTGCCGAC10.7633587786259541No Hit
GTGCTATTACTATGTTGTTTTTTTTTTTATTTTAGATTTATTAAAATGCCTGAATATTAACTGCTGTCTCTTATA10.7633587786259541No Hit
ATTCAGAACGATTTATTATTTATATTTTTATATTTATATTATTAAAATATTAAGATGACGAAGATACTCATTGTG10.7633587786259541No Hit
CACGGGAGTGGGCGGGGGCGTGGTGGTGCCATTGGTGCCATTCTGGTGCGGTGGCTCGTCATCAACGTTGCTCGG10.7633587786259541No Hit
ATCAGATACCGCCCTAGTTCTAACCATAAACCATGCCAGCTAGCATTTGGGTGTAGCCTGTCTCTTATACACATC10.7633587786259541No Hit
CCTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7633587786259541No Hit
CCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAAACAAATAAAAGCAGAACAGAGATCTAATTAAA10.7633587786259541No Hit
CACCTGGACGGACAGCTGCCAGAGCACAGCCAGGAGTCCGATGTACAGATCCATGCAAAGCAGGGCCACATCCAT10.7633587786259541No Hit
GTAGCAGATGAAACACTTTCCGTGCAGGTTGCGAAGCTTCTCGACATAGAGGTACACGCTGATCGTTAGGATTAT10.7633587786259541No Hit
AGAAAACGGTGATGGAGACGACGGAGGAGCCGCGCCTGGCGTTGGAGAAGCTGCCCAACCAAAAGATCACCTCAG10.7633587786259541No Hit
GCAATGGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAA10.7633587786259541No Hit
GGGTGGGTTGGGGGAGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGG10.7633587786259541No Hit
ATAACGAACTTTAATTTTTATATATTTTTTATATTTTTATTTTTATAATATTTATATTAATAATATAATAAATGT10.7633587786259541No Hit
GTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC10.7633587786259541No Hit
GCATTAGTGCTCTTAAACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGG10.7633587786259541No Hit
GTTTTGAGTATGTGGGGCAGGGGAAGAGGTTGATGGCAGCATGTGGCGGATAATAAGGGATTTGATCGCAGGGCG10.7633587786259541No Hit
AAAATATACAAATAAAAAAAAAATTAAACTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7633587786259541No Hit
CCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTTTCGCTTAAGTCTGTCTCTTATACACAT10.7633587786259541No Hit
GGCACCAGACTTGCCCTCCAATTGGTCCTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGA10.7633587786259541No Hit
ATTTTATTTTTTATTTTTTATTTATTTTTTTTTTTTTTTTTTTAAATTTTTTTTTATATTTATATTTTAATAATT10.7633587786259541No Hit
CCTTTAGCCCTTCCACCTCAAAACCTCCAAGCCTGAGGCCTATAACAACCTCAAGGCCTCTTTTTGCCACGCTTT10.7633587786259541No Hit
CTTCTATACAGATAGTTATTTTTATTAAAAATATTTTTTTTTTTTATTAAATTTTTAATAAAAATTTAAAATAAT10.7633587786259541No Hit
GTCCTCTTCCGATCTTTTTTTTTTTTTTATTATTTTTTTATCTTAATCGAAACTATGGGTGGTGGTGCATGGCCG10.7633587786259541No Hit
GGGCATGCGGCTCCCCAGAAGGAAAGGCCCGGCCGGACCAGTACACGCGGTGAGGCGGTCCGGCCAGCCCCCTGC10.7633587786259541No Hit
CTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAAATACCAACGTTTTAACCGCAACAAC10.7633587786259541No Hit
TTTTTATAATTTTATTTTATATTTATTTTTTAATTTATTGTTTAATTTAATTTTTATTATTTTTATAATTATATT10.7633587786259541No Hit
GACCTATTCCGATCGTATTATTTTTAAATATTAATATTTTATTAAAGCTCCAATAGAGTATATTAAAGTTGTTGC10.7633587786259541No Hit
GTGCTGTTGGGCTGGTTTGTTTATATTTTTGTTTTATGTTTGTTTTTTGTTTTTTTTTTTTGTGTTTTTTTGTTG10.7633587786259541No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATT10.7633587786259541No Hit
GTCCTGAAGAGGAAGTGGTTACGGCCACCACCTCGGCGCCAACAGAGGAAGACGTTAAGCCAACAACCGCCGGAA10.7633587786259541No Hit
TTTTTGTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTGTTGGATTATTATTTGTGAAT10.7633587786259541No Hit
GTCCGATTCAGATAATAGATATTTATTAAATTTTATTATTATTTCATAACAGACCACAAAATGACTCAAAATTCA10.7633587786259541No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTATATTTTTTATTATTATTATTTTTT10.7633587786259541No Hit
CATCGAATCAATATTATTTTTTAAATTTATTATTAAATTAAATAATTATAAATATTTTAAGAGAAAAAATGAAGG10.7633587786259541No Hit
TAGTACAACTTACAATTGTGGTTAGTACTATACCTTTATGTCTGTCTCTTATACACATCTGACGCTGCCGACGAC10.7633587786259541No Hit
ACACAAATGTGGTTTGTTTTGGTTTATTTCTGGTTACTTCTTCCTTTTGGTTTTTTTTGGTAAAATAAAACGTAG10.7633587786259541No Hit
GATCTTTGGTATTGTGGGATTTGGTTGTTGTTTTTTTTGGTTTTTTTTGGTGTTGATTTTGATGAGTTTTGTATT10.7633587786259541No Hit
ATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGCCCTCT10.7633587786259541No Hit
GCGGTGGGCGGGGGCGCGGTTAAGGGGTGTGTAGTTAGAGAGGCGTGGGTGTGTGTGGGAGACGCATCTGACGCG10.7633587786259541No Hit
GATATATATAATAAATTAATATTTTGATAATTAATAAATTTTATTGATATATAATATATAAAATATATTAATTTA10.7633587786259541No Hit
CTTCCGGGCTTTTGGTCAAGATGTTGGAATTTTAGATATTATATTTTAAGTTAAAGTTTTGAGGGGTGGCTGGGG10.7633587786259541No Hit
ATACCGATCTTATAATAAATTATATATTTTTAATAATATTAGTCGACCACATGATATTGATAAATTTACAAATCA10.7633587786259541No Hit
CAGGACTACTGTACAATTTAATTATTATTTTAAAAAAGTAAAACATCACCGAACGACCGGCCTTTTAGCATCTGT10.7633587786259541No Hit
CTTGTATTTTTTCATATGTTCCTCCTATTTAAAAACCTGCATTAGTGCTCTTAAACGAGTGTTATTGTGGGCCGG10.7633587786259541No Hit
ATATTATCTCTTGGGTGTACTAACCGCTCGTCATGGGTTGAGAGGTTTTTGGATATGAAGATGAGTAGGGTAAAT10.7633587786259541No Hit
CTTCGGATAAATATAAATTTTTTTTTTTAATATTTTTAACGGTGGACATCTTATATCAGATTAAATAATTGAATA10.7633587786259541No Hit
CTTCAGATCTTTTTTTTTTTTTATTATTTTTTTTTTTTTATTTATTTAAGAAGTTTTTAAAAATTAGTATTACGA10.7633587786259541No Hit
AATTTATTTTTTTTTAATTATATTTATTTTTGATTTTTTATTTTATTGTAAAGAATAAAGATATTTAAAAACACT10.7633587786259541No Hit
CTTCGGATCATTTATTTTTTTTTTTTTTTTTTTATTTATTAATGGATCAAATACGGTCTGTTATAAAAAAAAGAA10.7633587786259541No Hit
GGTTGGGGTGGCGTGGGGCGGTGCGGCGGAGTGGGGGGTGGGGGGGCGGCGGCGGCGGGGGGGGGGGGCGGGGGG10.7633587786259541No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAA10.7633587786259541No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG10.7633587786259541No Hit
GTACACAAACTAACAAATATTAAAATTATTTGTTTATATATTTTTTATTATTTAAATTATTAATATTAATTATAT10.7633587786259541No Hit
CTTCCGAACTTTTTTATATATTTTTTTATAATTTATAATACTCATACTCAAAAAAAAAAGTGATTAAATAAACAT10.7633587786259541No Hit
AAGAACTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7633587786259541No Hit
AGCGGAGGCCGGGGGGTTCTAGTGTGGTGGGGTGGAGGATGGCGGTTATGTTGAGTGGGGGGGGTGGGGGGGGGT10.7633587786259541No Hit
TCCCATGAATATCACTGAACAGCGACCCAAATCGAATGCTTTTGGAGCGGCAACGTGGAAGATATTTCAATCCTC10.7633587786259541No Hit
CAGGTGCTCTTCCGATCTATTTTTTTTATTTTTTTTTTATGTTTAAAGCTCAGAGCACGCGATCAATAGACAGCA10.7633587786259541No Hit
TGAGTACACTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGC10.7633587786259541No Hit
AACTTGTGCTTCATACGGGTAGTACAACTTACAATAGTGGCTGACTCTTATACACATCTGACGATGCCGAAGACT10.7633587786259541No Hit
CTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCCTGTCTCTTATACACATCTGACGC10.7633587786259541No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTAGGGCGCCGGGGGGGGGTGGGGGGGGGGGTTGAGGC10.7633587786259541No Hit
CAGCAAGCATGTATGCTGAAGTAAAGTTGCCATCCCACTTGTCCTAGCAAGCACCGTCTTTTGTGCTGAAGTTAT10.7633587786259541No Hit
GTATAGGAGTTGAGGGCTCATCGGAGGTCACGAGGTGAGGGGGTGTGTGTTATAGGCAGCTGACGCTGGGGGCGG10.7633587786259541No Hit
GTTGCGAGGGCGCGCGGGGGGTGGTCGAGGGTGCGGGGCGGGGGTGGGCGGGGGGGGGGGGGGGGGGGGGGGGGG10.7633587786259541No Hit
ACAATGATACATTTATTTAATAAAAAGAAATACCACAAGAGGAAATAAACTTACAAACAAATACGTATAAAACTA10.7633587786259541No Hit
GGAGGAGACCGGCCGTGTGCTGAGCATCGGTGACGGTATCGCCCGTGTCTACGGTCTGAACAACATCCAGGCCGA10.7633587786259541No Hit
GAACTAATAAGAAAAAGAAGCAGCAGAAAAGGCGACGTAAGAAATTCCGGAGTTTTCATTCGCATTTTCCAGTCT10.7633587786259541No Hit
CTCCGCTTACTACGGTTTGATTATTTTTGGTATAGTTATAACAACAATTGAATGTTGTAATAACCGTATGAAGCA10.7633587786259541No Hit
CACATCAGATAATATTTTATTTTTTTTTTATTTATAATAAAAAAATAGATTAGAGAAATAGCTATGAAGCTGTCG10.7633587786259541No Hit
CTACATAGTCAAAGGAACCCTGTACATTCCGTACGCCGAGATTGCGGAACCCTTCTACTGTCTCTTATACACATC10.7633587786259541No Hit
GTGGTGGGGGGGTCGGGGGGGGGGGGGGGGGGGGGCGGGCGGGGGGGGGGATGGTGGGGGTGGGGGGGGGCTGGG10.7633587786259541No Hit
GTGCTCTTCCGATGATTTTTATATATTTGTTTATTAATTATTTTTTTTAGCCTGTTATAAGGCCTGTCTCTTATA10.7633587786259541No Hit
AAATTGTGATATGGCGATCGAATTTGATTTAATATGTTTTGAGTACTGTATCTGTCTCTTATACACATCTTAAGC10.7633587786259541No Hit
TAAATATACCAAGCAAATATTATTATTTATTTTTAAGCCTGCTTAAAGCACTCTAATTTGTTCAAAGTAATAGTA10.7633587786259541No Hit
ATTATAAGGCCACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTGAGCAATAACAGGTCTGTG10.7633587786259541No Hit
ACAACATTTTGTTGATGCGAATGACGTGGAATGGATGCAGGCGCATTCTGATGTGGAACTGGTCCTTACCGCAGT10.7633587786259541No Hit
GTTCAGATGCTGTTGTCGAACAAGTTCCTGTCTCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGC10.7633587786259541No Hit
TGGTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG10.7633587786259541No Hit
CCAATTACTTAAATTTTTTAATTTATTTTTTTTTATATAGAATTACCACAGTTATAAAAGTAAATGAAAAAGATA10.7633587786259541No Hit
ATACCGATCTTTGTTTTTTATTTTTTTTTTTTTTTATATATAGCAATTGGTTGTAGATAAATTTATTGCCTGGCG10.7633587786259541No Hit
CCACGGACTTGACCTCAGTGGTGATGTTAGCAGGGGCGAAGACCACAACGGTACCGGGCTTCAGCCTGTCTCTTT10.7633587786259541No Hit
CTTCCGATCTTTTTTTTTTTTATTTTTTTTTTTTTTTTATAACAGTGTGTAAGTCAAAATAAGTAAAAAGGACCA10.7633587786259541No Hit
GTGCTCTTCCGATCTAAGAAGGGGAGACGTGTGCTCTTCCGATCTCCAGCGGGAGACGCTGTCTCTTATACACAT10.7633587786259541Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATACCGATCATATTAATAATATAAAAAATAATAAAATTAAGCCGCCCCCAACAAGGAAAAAAAAAAACAGTTGAT10.7633587786259541No Hit
CTTCCGATCTAGTACGGGTTGGATTGATTGAAAATAACTACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAAT10.7633587786259541No Hit
ACATTAATATACGCTATTGGAGCTGCAATTACCGCTGCTGCTGGAAACAGAATTGCCCTACAATTGGTCCTTGTT10.7633587786259541No Hit
GTGCTCTTACCGGCTTGAAGTTATTTGGTTTTATGATTGAGCGTAAATTACCAAATACCAGCTGTGGCGCTTAGA10.7633587786259541No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers