FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length75
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCGAAGGCGTCCGCCCAGAGGGCGAGCTTGTCGGGATCCTCAATGGCAATGTCCGTGCCACCGGGGCCGAAA13.0303030303030303No Hit
GATTTGGAGAATTTCAAGCGAATCGCGTCGTGAGTCACATGGGATCGGCGCTTGTTTTTGTTTTGTGGGTTATTG13.0303030303030303No Hit
GGTTGTTTGTTTGTTTTTATTTGCAGGGCCGTCCGGGCGATGTAAGCCGATGGTAGCAAGGCGCTGAATGAGGAG13.0303030303030303No Hit
CTTCCGATCTGAGCAGGGAGACGTGTGCTCTTCCGATCTGAGCAGGGAGACGTGTGCTCTTCCGATCCAGTCTCT13.0303030303030303Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAATTAAAACATGAACCT13.0303030303030303No Hit
ATCTTGTAGCCAGTGAGCTTGCCATTGGGATACAGCGGCTTCTTCCAGTGGAGCATGAAGGCCGAGGAGCCCAGA13.0303030303030303No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13.0303030303030303No Hit
AGAAACAACATATTGTATTTATTTTTATATTTTATTGATTTGTACATTCTGACAACCAACATCAAGTTTGTACCT13.0303030303030303No Hit
GTCGGGTGGGGTTGGTGTGGGGGTGGGGTGGGGTGGGTGGGGGGTTGGGGGGGTTGTGGAGGTGGGGGTGGGGGG13.0303030303030303No Hit
CCTAAAAAGAAACCGCCGCTACTCCTTGTGTACATCTACTTAGACGCAATATAAGAAAAAAAAAAACCGAAGTTT13.0303030303030303No Hit
ATTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCTGTCTC13.0303030303030303No Hit
GGCAGGCAGGCAGGCTGGGCCTGCTCCTTCTGTTCGACCACCTTTGGGTCATAGTCTATGTGGGCTGTCTCTTAT13.0303030303030303No Hit
GCTCTTCCGATCTCACGAGGGGCGACAAGGGCGGGCGACTGACCGCCTGGCTGCGCGAGCACGCCTGGAACGAGC13.0303030303030303No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTGTTTTTTTTATTTTAATTAATGGATGCTGTCTCTTATAAAAATAAAACG13.0303030303030303No Hit
CAATAATGTGATGGTTAAGGCCACAACCCTGTAGCCAGACTGCCTGATTTCAAGCCTTAATTCCAACATTAACTA13.0303030303030303No Hit
GTGTAGATCTCCTTCATCACCCAAACATTAAAAAAAACAAGCGTCTTGTCCACATCAGCCATGCTGCTTACAAGT13.0303030303030303No Hit
GATAAAAAACGAAATTAATAAATTATAATTTTTTTTATTTTGATTTTATTAGGCAAAAAAATGTATATTATACAA13.0303030303030303No Hit
GTTTTAAGATCACACTCAAAAACAACATCCAAAGTGGTTAGTGGCAGTTGAGAGAGTGCCCAAGACCCCGAATAC13.0303030303030303No Hit
CTTCGGGGCGTTTTTTTTTATTTTTTTTATTTTATGATTTTATGTGCCGAAGTACAAGGACGAGATTACCGACAA13.0303030303030303No Hit
GCCTAAGAATACGAACTTTGTTAAGAAAATTGAAGAAACGATTGCTGACTCTTATACACATCAGACGCTGACGAC13.0303030303030303No Hit
TCCCGGGGATTTACGATGATCGAGCTGATGATCGTCGTCGCGATCATCGGGATCCTCGCTGCCATCGCCGTCCCG13.0303030303030303No Hit
TATTGGGACGAAATTAGAGTGTGGACTGCACTGAATAATTTGTGGAAAACGAAAAAAAAACATCCTGTTTGGAAT13.0303030303030303No Hit
TTGCTCGCCATTCTGGTGCTGGGGGTTGGTTTGGTGCTGGTTGCCAAGGCTGGAGAGCGAAGTATGGCGCCAAGG13.0303030303030303No Hit
GATCTATTTCGATCTATTTTATTTTTTTATTTTTAATTAGACAGATCTTTCAAATGCTGTCTGTTATACACATAT13.0303030303030303No Hit
CATCAACCGAGATTCCGAAAGTAGTGGCGAGCGAAATCGGAAGCGCCTGTTAGTGATAGCACAAGACATAACGGA13.0303030303030303No Hit
CTTAAGATCTATTTTTTTTTTTTATTTTTTATTTTATTAAAAATACAAAACCTTAGATTGAGAATTAAAAAAATA13.0303030303030303No Hit
CCGCATCTTCACGGCGATTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCTGGCCATCATTATGCCTGTCTCTT13.0303030303030303No Hit
GTGCTCTTCAGATCTGGACTGGGGCATTGTGTTCGCGACGCATTTATGTTCGCTTACGCCACATAAAATGAAAAA13.0303030303030303No Hit
GTGGGGGGGGGGGTGGGGGGGGGGGGTGGGGTGGGGGGTGGGGGGGGGGGGGGGGGGGGGGCGTTGGGGGGGGGG13.0303030303030303No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13.0303030303030303No Hit
GGGAGACGTGTGCTCTTCAGATATTAAACCTGGAGACGTGTGATAATAACAAATTACTCAAGAACACGTTAGAAA13.0303030303030303No Hit
GTGCTCTTCCGGTCGGTGTTGGTGTTGGTTTTTTTTTTATTTGTGTGTGATTGGTGTATTTGTTGTTTGATTTTT13.0303030303030303No Hit
TGTCCCATAAGGTTCATGTTTTAATTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAACTGTTAACG13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers