FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length75
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGATCATATTTATATTTTTTTTTTTTTTTTTTTTTTATTATAGATAATAACAAATATAAACATCAGACGAT12.941176470588235No Hit
GGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.941176470588235No Hit
GTGCTGGTCCTGTCTTATTTTTTTTTTTTATTTTTTATTTATAAGAATTTGTGGTCTATCGTGGTTATTATAAAG12.941176470588235No Hit
GGGGGGGGTGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.941176470588235No Hit
CCATACTTCACCAGGAGCCCAAAAGCTTTGGTTTCAAGGGAATATAATGATAGAGCCATAAAAGTAGCTACAAAA12.941176470588235No Hit
GTCTTAAGATGTATATTATTTTATTATTATTTTTTTAAATGAATTTCACCTATCGAGTAGTAATTCTAATGAGCC12.941176470588235No Hit
GGGGTAGGGGATGGGGCGGGCGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.941176470588235No Hit
ATTACGGAGAGGGAGCCTGAGAAACGGATAACACAACTAAGACATTATCATTTACAAATCTTACCCTCCCAACGA12.941176470588235No Hit
GTGCTGTAGTGGTGGGTTGTGTATAGTGTTTGTATGTGTTTTGATTTAGTTTTTGTTGGATATGTCTCTTATAAA12.941176470588235No Hit
GTGCTCTTACGATCTTTTTTTTTTTTTTATATATTTATTTATTATTTATTTATTTATTGAGTAATTAAAAAAGCT12.941176470588235No Hit
CGCTTAAGTTAGTGTTTGGAAGGTGTTTGATTTTATAATGTAGAGTCGTAATAATTATTGGAGGAAACTGGTTAT12.941176470588235No Hit
GTGCTATTAAGATCTTATTATATTTTTTTATTTTTTTTTTTTTTAAAAAAAAAAAAAAAGAAACGGATAATTTAT12.941176470588235No Hit
GGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.941176470588235No Hit
GAGCGGATCGCGTCTTTGTGTTGATTATTTTTATATTTTATATATTTTGATTATAGATAAAGGCAGTCACCAAGG12.941176470588235No Hit
GGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.941176470588235No Hit
TCCAGAAGCAGACCCGTGCTGGTCAGCGTACCCGTTTCAAGGCCTTCGTTGCCATCGGCGACAACAATGGCCACA12.941176470588235No Hit
CATATGTTCCTCCTATTTAAAAACCTGCATTAGTGCTCTTAAACGAGTGTCTGTCTCTTATACACATCTGACGCT12.941176470588235No Hit
TTTTTATTATATTTATTTTTTATTTATTATATTATTTTTATTTTTTTATATATTAATAAAATTGTTTAAAAGTTT12.941176470588235No Hit
GCATGGGGAGACGTGTGCTCTTCCGATCTGCATGGGGAGACTGTCTCTTATACACATCTGACGCTGCCGACGAAT12.941176470588235Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTAATATTACGAACATATTTTATTTTAATTTTTTAATATTTAATAAATAAATGAAAAGAAATAAAAAAAATTGTA12.941176470588235No Hit
CTAACGAGCATATAATAATATTTTTTTATTTTTTTGTGAAAAAGCAATCTCAAATAAAAAAGACGGAAAGACACC12.941176470588235No Hit
GTGATGGGCCGATCTGTATTTTTTTTTTTTTTTTTTTTGTCTTGTACCGACGACAGATGTTTGAAATTTATGCCC12.941176470588235No Hit
AATTAGAGTGATTAAAGCAGTCTTCAAATGCATGAATATTCTGTGCATGGGATAATGAAATAAGAAATCTGTTAT12.941176470588235No Hit
GTGCTAATCCGTCTTATTTTTTTTTTTTTTATTTATTAATATTATTTTTTTTTTATTTTTAAATATGAAATAATA12.941176470588235No Hit
CTATTTAAATTTTTTAATATTTAATTATTTAATCACATAAAAGACAGACACTTATACACATCTGACGCTGACGAA12.941176470588235No Hit
GAATTTCACCTCTCGCGTCGTAATACTATTGCCCCCCAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAAC12.941176470588235No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAACAACAAAGACAAAAAGTAAAAATATATA12.941176470588235No Hit
GTTCTCGTAATTGATGTGGTTTTAGTTGGATTTTTTTATTTATGATTATGGGACATGTGCTTTTATTACTGTCTC12.941176470588235No Hit
AGATCACTGTGCGCGGCCTCCCTTCCCTTAACAGCTGTTCCTATGACGGCTGGGTCACATTCTAATGAGTAATCT12.941176470588235No Hit
CTTACTAAGAATTATTATTTTATTTTTAAATATATTATGCAATGAGCAACTCAGAATTATTTCATTGCAATTGCT12.941176470588235No Hit
TCCCACTGCAGATCGGAAGAGCACACGTCTCCCACTGCCTGTCTCTTATACACAACTGACCCTCACGAGGAATAT12.941176470588235Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGCTCATCCGATCTTTATTTATTATTAATTTTATTTTTTTTTTTTTTTACAAAAAAATATAATGAGAGAGCAAT12.941176470588235No Hit
CATTAGGAACAAGGCCCGCAACGATGAATCCGGATCGGATGATAGCGTGGATGAGCGCGAGATAGATTTGAATAA12.941176470588235No Hit
GCTCCCGACTGTAATCCGTGCACGTTGGGAGGCCAATGCGGGCAAATGACCGGGGGTGTGGGGTGCGTGACGAGG12.941176470588235No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers