FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length75
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAATATATTTATTTTTTTTATATTAATTTAAAACGCTTAAATAAGTGTATATTAAACAAATCTGACGCTGACG13.125No Hit
GGTTTTTTTTGTTTTTTTTTTTTTGTTTGTTGTAGTTTTTTTTTATTGATTAGTTGATTTGTTGTTTGTTTTATT13.125No Hit
CATCCGGTCAAATATTATTATTAATTTTATTATATTTAATAAGGACAACAACCCCAGGGAGCAGTTGGAAAGAAA13.125No Hit
TCCTGGTACCCAATTCGTTGAGTTTTCGCACTGCGAGAATTACCTAGTTACTTACGGACCGACACCCACGGGCCA13.125No Hit
ATCCGTTGAGCGATGGCCCACCCACGCGGGACCACCGGATCACTATGACCGACTTTCGTCTCTGCTCGACGTGTC13.125No Hit
GCCAAGAAAATCGCTTGGCTTAGGCTTCGCCTCTTGCCCGTAATTAAAGCTGTCTCTTATACACATCTGACGCTG13.125No Hit
GTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGA13.125No Hit
GTCTTGGCAAAATAGATATCTTTTTCGATGCCTGCATTAACTTCAGGAGGTGATTCTAGGTGTATGCTGTCTCTT13.125No Hit
CCTTTAAAAAGGACGATTTGTAGGGAAATTATGGTGAAAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATT13.125No Hit
CTTGTGAACCATTTTTATTTTTTTTATTTTTTTTTTTTTATTATTTTTAAAAGAGATCGAAACATCGGTATAGTG13.125No Hit
GTGATCTTCCGATCTTTTTGTATTTTTTAATTTTATTAATAGTGATGCGCTTAATAGTCATATTAAACAAGGGAG13.125No Hit
CCCATAGATTCGAGAAAGAGCTATCAATCTGTCTTACAGACTTATGCTGTCTCTTATACACATCTGACGCTGCCG13.125No Hit
GTGATATTCCGATATATATATTTTTATTTTTTATATTATGGTCTTGTAAATACGACAGATCGTTAAAATGTCTGA13.125No Hit
TCTCACACGCCCCTCTTTATGGGTTGAAGAAGTTAGTGTGTTTTCTCCCTTCTGTGTGTCAATTAAAATGGTTGG13.125No Hit
GTTTTAGTTGTTTTTGTGTTGTGTTTGGTTGTTATTTGTTTGTTTTTTGATTTTTTTTTATTTATTTGTATTTTT13.125No Hit
GTTTTAGTGTGGTTTGTGTGGTGTTTTTTTGTTGATTTTTAGTATGGAGGAGTGGTAGTGAGTGGAGTAGTGTTT13.125No Hit
GGGGGGAGGGGACGAGGGCGGGGGGGGGGGGGGGGGGGAGGGGGGGGAAGAGGGGAGGAGTTGGGCGCGGGGAAG13.125No Hit
GCCTGGAAATATGGAAAGTTTGTCTGCGTGTGGGCTGCGTGCTTCTGCACTGCTGTGCGTCTGTCTCTTATACAC13.125No Hit
AAAAACACCTGTGCTCTAAAGATATGGTTTAAATTTTTTATAATTTTATATAAATAAAAGTAGGTTCAAAAAAGT13.125No Hit
TTTTTTTTTTTTTTTTTGTATTTTTTTTTTGTTTTTATTTTTTTTTATATTTTTTATTTTATATTATTTTATATT13.125No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACCGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC13.125No Hit
CTAAAATTTTTTTTTTATTTATTTATTTAATGTATATTAAAAAAAAAAAAAGTAAGTTAAAATAAAAGTATGAAT13.125No Hit
AATAGGATCTTTTTTAGTTTTTTTATTTTATTATTTTTTAAATTTTATTTTTTAGTTAGAATATGATATAGTTTT13.125No Hit
TCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTATTGCTA13.125No Hit
CTTGCGATCTTTTTTTTTTTTTTTTTATTTTTTTAATATAAAATAAATAAAGTTTTAAAAATAAGCAGAATGGTC13.125No Hit
CGAACAAACTATGTTTTTTTTTTGGATTGCAGTCTTGGAATTTAAAGAAGAAACGGTTGGTTTCAAGAATGAGAC13.125No Hit
GATTTAAAGAGTACTAAATCCAATTACAGGGCATCGGATATGAGTCATGGATTATTAGTTTGATTAACTACCTCC13.125No Hit
GTGCTCAGCCCATCTTTTTTTTTTTTTTTGAATTAATAAAAGAGATGGGAGTTGGAAAATTACCCCGCTGCGGCC13.125No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT13.125No Hit
GTAATTAGCTGCTCAAAACAGTTATTCTTACATCTATGTGACAATTTTTAGCCAAGTTATAACGAAAATTTCGTT13.125No Hit
CTTCGGATCAATTTTATTTTTTTTTTTAATAATTTTATATCGCCTTGACGCGAGGACGTAGGAATAAAACGATCG13.125No Hit
AATGATCTCACAGCCGCCCTGTAGCTGCTACAACTAATTACCTCCGCTTCGAACGAACCGGACAACAAAAAATAA13.125No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)