FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68
Sequences flagged as poor quality0
Sequence length75
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGGGCGCGTGCGGTTACACCACGTTTGACGCGAGGCATGGATGGACTCCTGGTTCTGTCTCTTATACACATC11.4705882352941175No Hit
ACAGAGAGTTCGACCAATCTCAGGAACTTGCCACCGCCTGGTTATAATCCAAATAAAGCACTTCACCCTATAGGT11.4705882352941175No Hit
ATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTTCATTATCCCATGCACAGACTGTC11.4705882352941175No Hit
GTCGTCGGTGGGGGGGGGGGGGGGCTGCATGGTGGGGAGAGGTGAAGCAGGAGGGCGCGGGGGGGGGAGGGGGCG11.4705882352941175No Hit
TTATTGGACCGGGGCGATTTGTTTGATAAGACAGCGCTATCGCATTATCGTGATATTGGGTGGGCGCTGGCTATT11.4705882352941175No Hit
GTTCTATTCATAAATTTATTTTTTTTTTTTTAATTATTATTATCATACATATGACTGAAAATAAAAAAAATTATA11.4705882352941175No Hit
CTACGGGGATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATGCTGTCTCTTATACACATCTGACGCTGCCGA11.4705882352941175No Hit
AAGCTGTCCGCAGAGGGGGCCGCTCCGAGCCGTGAGACCACTTTGGTGTCACCCCAGGATCTTAAGGAGATCAAG11.4705882352941175No Hit
TGCTCATCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTATTT11.4705882352941175No Hit
CAATATACAGCAATATAGAGATCTTAGTTATGACCTAATAAAAAACACTCTACGCTACAAGTCCATTGTATCTGC11.4705882352941175No Hit
TCTAATTACAAACGAATTAAAAGTGAAACATAAAAAGGATCATTATATAAGTTATGTTTAATGTCTCATATACAA11.4705882352941175No Hit
GCATTTTAATACGTTGAGATGGCTACTGTACGATGAATTCTTTAACTAAGTTTAACTTTAGCCGTGACCGCGAGT11.4705882352941175No Hit
CATACGAAATAAATATTTTTTATTATTTTTTTTTAGTAAGAAAACTTGAAGGAACCTAGCCCTCGAAGCCCTGCT11.4705882352941175No Hit
AAGCCAACCCTAGTACGAGAGGACTGGGTTGGGCCAACCTATGGTGTACCGGTTGTTATGCCAATAGCAGCGCCG11.4705882352941175No Hit
ATAACCTGCAGAGCGTATGGTCTTGTCCCGACGCCCGATCTTTCAAATGGCTGCCCTAGCACCGGTTGATGGTAG11.4705882352941175No Hit
GATCTATAGGTAACAAAATTTAAATATTTTTATTTATATTTTTTTTAGACTAAAAAAAGAACATGTCCAATAAGG11.4705882352941175No Hit
GTGCTGTACCGATCTTTTTTTTTTTTTTTTATTTTTTTTTTGGTTGGATTAAGGAGATTGAGTATGAGAAACGGC11.4705882352941175No Hit
TTTTTTTTTTATTTTTTTTTTTTTGTTTTTTTTTTTTTATTTTTATTTATTTTTTTTTATTATTTATTATTTGAT11.4705882352941175No Hit
GTTGCGGTTAAAACGTTAGTAGTTTAACTTGTGCTTCATACGTGATGTCTCTTATACACATCTGACGCTGCCGCG11.4705882352941175No Hit
TATATCGTCAGATCGTTTTTTGACTATTATTTTTAAACTTTGTTAATATAGTGTGTGTGTGTGAGACTGTGGTGA11.4705882352941175No Hit
CTATTCGAAATTACCAACTTGATAGAGTATTATAGAAACAATTTCAATGGAATTCTGCTCAACTTAAAATGCTGA11.4705882352941175No Hit
GATCTGTTTTTTTTTTTTTTTTTTTTTTTTATTTATATTTTATTATTTTTATATTTTATTTTTTTAATTTAATTA11.4705882352941175No Hit
GCCGTTGGCCGATCTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGGTGGGTTTAAAAACATAGAGGGGTGGGG11.4705882352941175No Hit
AGTTAGGACGATTCATCTAGTTCATGGTGGAACTCAACGTCGTACATTGGAAACAGTACGTTCGAATTGAAGATT11.4705882352941175No Hit
CTTCGGATATATTTTTTTTTATTTTATTTTTTTATTTTTTAATAGAAGCTGTTTGTTGACATTATTATTAAGAGG11.4705882352941175No Hit
TCCCATCATTTTTTATAATTTTTTTTTTTTTTTATAAAGATGGTTGCACCCTCCCACGGAGTCAGAGTAAAAGAC11.4705882352941175No Hit
CTTCAGAAGTTTTATTTTTTTTTTTTTTTATTTTTATTATTTTTTTTAATTTTTTATTTTAATACAATTGAAAAG11.4705882352941175No Hit
GTGATCTTCAGATCGTTTTTTTTTTGTTTTTTTTTTATTTATTTGTATACCGATATTAAATTAAATACAAGATGG11.4705882352941175No Hit
TGCGATCTTTTTGTTTTTTTTTTTTTTTTTTTATTGAAGGGCCACAGGAAGTTTTAGGCGTACTTGAAGTTGGGC11.4705882352941175No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.4705882352941175No Hit
ATTTTATGGTCAAGTTCCAAGAAGTGAAATGCTTAACAAACAACAAAAACAAAACACACAAAAGCAAGCAAATTA11.4705882352941175No Hit
CTTTAGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATAAATAAATTAATTTTAGAAATATAATAATAGT11.4705882352941175No Hit
CCAGTACTAGCATGAGCTATTACTATATTATTAACCTGTCTCTTATACACATCTGACTATGCCGCAGAGATGCAG11.4705882352941175No Hit
TATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACTCGAGAGTTGAAATTCATGTCTCTTATAAACATCTTA11.4705882352941175No Hit
ACAACATAAAGGTATACGACTACCGCAAAATGTAAGATCTCGTGCCCGTAAGAATCAAAACATCATAAACCACCG11.4705882352941175No Hit
ATATGGATCTTATTTTTATTTAAATATATTTTAATTTATTGCGATCGCTTTGTAAAAGACTAAAAGCACAGGTCC11.4705882352941175No Hit
TCCCATGTCAGATCGGAAGAGCACACGTATACAATGTAAGAACTGAAGAGCACACGACGACAATGATAGATATGT11.4705882352941175Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTGCACAACCTACGAATTTTGTATATTTATTTTTTTTATAATTCATTAAAAAAAAATAAGAAATTACACCTCACG11.4705882352941175No Hit
AATCCGATCTTTTTTTTTTTTTTTTTTTTTTTTATTTAGTTTTTAAAAAAAGCAGGCTGCGGCGGCGATTAGTGA11.4705882352941175No Hit
ACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACTACCATGGTTGCAACG11.4705882352941175No Hit
ATCCTGCACATAGTACATTGCGCCCCGATTGAGTCGGATAATGCAGATGATGATGAAGAGGATGAAGATGAAGGC11.4705882352941175No Hit
GTGCTCTTCCGATCATTATTATTATTTTTTTTTTTTATATTCGTCGAACACCAAGGCGAACTTCGGATTTAACTA11.4705882352941175No Hit
GGCATTTGAAGTCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGCTGTCTCTTATAAACATATGACGCTGAA11.4705882352941175No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCAGTTT11.4705882352941175No Hit
CCCTATCAACTTTTGATGGTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCC11.4705882352941175No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTCAAACCGCAAAAGGCT11.4705882352941175No Hit
TGTTAAGACAGCTTGACAGAATTATATCGATAAGATCAGCAACTTTCATTAAATTGCATATTTCACAAACTCGCA11.4705882352941175No Hit
GTGGTCTTCGGATCTGTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTAGGTATAAAAGAAGAGCAG11.4705882352941175No Hit
GTTCGGATTGGTTTTTTATTTATTTTTGATGTTGTATTTTGTGTGGATGTATTGTTAATGATGCGGTGGTTATTA11.4705882352941175No Hit
TTCTTTATATTGCATTATTGCCCTGGTAAGAACTTCCAACACAGTTTTGAATAGGAGTGGTGAGAGGGCATCCTT11.4705882352941175No Hit
CCAAAATGCCTGTGGAAGAAATTAGAGGGCGGACTGATGCGCATGATTGTGATGGGTCGGAATATCACGATCTGC11.4705882352941175No Hit
CTTATTTATTATTTTATTTTTTTTTTTATCATAACCTCAGGCGCTGAAAAGAAATGAAAGAAGAACCGAGGTCGT11.4705882352941175No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTATATTTTTTTAATTTTATTATTAAAAATAAAAAA11.4705882352941175No Hit
ATTCCGTAGAGTTATAATTATATAATTATATAATATTTTATTTATTAATATTTATTTTTTTTTTAAATATTAAAT11.4705882352941175No Hit
TTCCAGCTCCAATAGAGTATATTAAAGTTGTTGCTGTTAAAACGTTCGTAGTTGAACTTGTGCTTCATACGTGTA11.4705882352941175No Hit
ACTTTAGTCACATATAATGTGTAGACGGTGCGCTCGAGGATTTTCTTGATCTTGTCATCGTAGGGACATTTGACA11.4705882352941175No Hit
TCTGAGACGTGTGCTCTTCCGAAATTATAAGTGTCCAATAACAAGCTGCCGAGAATAATCTGTGTCTATTTCTCG11.4705882352941175No Hit
GTCCTCTTCCGATCTGGTTTTTATAGTTTTTTATTTATTTTTTTTATTTGTTTAGGAGGACAAATAAATCTGTGG11.4705882352941175No Hit
GGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGATATGC11.4705882352941175No Hit
GCACAGCTATTTGTGGTGTGTTATTGTGTTGAATTTTTTAGATTATTAAGATATAATAATGTTAAGGGTTTTAGT11.4705882352941175No Hit
AAATGAAACAGATGAAGAAAAATGCAAGACACAGGAGCCTGCTACTCCATCCTCGTGGATAAAAACTGTTCTGAC11.4705882352941175No Hit
CTATTCAGAAATATATTATTTTTTTTTTTTTTATAATTTTTTTTATTTTTTTTTTAATATAAAATTTTTATTGTA11.4705882352941175No Hit
CTTGCGATGTATTTTTTTATTATTTTTTAATGTGGTGTTATTATATGTGATTAAATAAATGTCTCTTATACACAG11.4705882352941175No Hit
GACAAGGGCATCCGGTCTGTTTTTTATATATATTTTAGTATATTTAGGGCTGGACAGGAGTTTGAATAGCAAGCG11.4705882352941175No Hit
GTTATTGCTCAATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAGCTAGTGTCCTTATAATGGGACAAAC11.4705882352941175No Hit
GATAATAATTACTAAATAAATTATTTTATGAATTAATTTTTTATTATTTTTATTATGCTACCTTTGCACAGTCAA11.4705882352941175No Hit
TTGTTTTTTTTTTTTTTTTTTTGTTTTTTTTTATTTTTATTTTTATTGTTTTTATTTTTTTTTTTTAATTATGTT11.4705882352941175No Hit
CTCCTAATCCGATGGATAATTTATATAATTATATTATTAGTCGACACCCAGAAGAGCGATTTGATAAATATAGTG11.4705882352941175No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCC50.069.056
ACCGGTT50.069.048
GGTTGTT50.069.051
TTGTTAT50.069.053
GGTTGGG50.069.027
CCAACCT50.069.034
CCAACCC50.069.04
GGGTTGG50.069.026
CCAATAG50.069.061
CCTATGG50.069.038
AGCCAAC50.069.02
GAGGACT50.069.019
AGGACTG50.069.020
CTGGGTT50.069.024
CAACCTA50.069.035
GTACCGG50.069.046
GGACTGG50.069.021
GTGTACC50.069.044
CAACCCT50.069.05
TATGCCA50.069.057