FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGCTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGAGTTTAGCTTCTGCGAAGCTACGAATCGAAGCC10.8064516129032258No Hit
TTGTTAAAGGATTTAAAGTGTACTCATTCCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGG10.8064516129032258No Hit
ATTACTATGTGTCCGCCAGTGCCAAGGGAAACGTGGAGATCAAGGAGATCGATCCCGAGGGCAAGTTCGTAAGGC10.8064516129032258No Hit
CTCTACGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCATCTTCACAGGTACTATGATTTCACCGAGTCTCTCGT10.8064516129032258No Hit
CCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATT10.8064516129032258No Hit
AAAAGTGCGCGGTCCCGTATGGTCGAGATAGAACAAAACAATACACATAACCGACGCCGTTAAAAGGGCCAGGTA10.8064516129032258No Hit
GTTCTGAGTCGAGGGTTCGACGCCCGGGGAAGACCGCCGAAGCGCTCGTTCCCTGTCCGTCCCCGGGCCGGCACG10.8064516129032258No Hit
AAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT10.8064516129032258No Hit
ATAATAATAACGACACACAAAAACACTAGTATAAAACAACTACTTAAAATATATAAAAACCAGCAAAATATCAAA10.8064516129032258No Hit
ACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAG10.8064516129032258No Hit
AGCAGCAGCTGCGCCAGCTGGAGAAACAGGTGGGCGCCTCGTCGGGATCTTCGGGCGCAGTGTCGAAATTCGGAA10.8064516129032258No Hit
ATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTATATTGGTTGAACTCTAGATAACATG10.8064516129032258No Hit
GGACACGAATGTGACGCTGACGGATCAGGACGCGAGCATGATGGATGGACTTGGCAAGTCCCAGCTTGAACACCT10.8064516129032258No Hit
GGGGGGGTGTGGTCGGGGGGGGGGGGGGGGGGAGAGGGGGTGTGGGGTGGGGGTGTGGGGGTGGGGAGGGGTGGG10.8064516129032258No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGGTCCTTGTTAAAGGATTTAAAG10.8064516129032258No Hit
TCTTTGATTTTATATTATTTTTTTTTATATTTATATTATTAATAATTAAATAAAAAAAACTATAAAGCTACAGCC10.8064516129032258No Hit
TTTTTTTTATTTTTTTTTTTTTTTTATATTTAAATTTTTTTTTTTTATTTAAAATTTATATTATAAAAATATGAA10.8064516129032258No Hit
GTTTTAAGGTTCCTGAGATGAAAAACATTGACATTGTTAACTGTAACTATTGATAAAGTATTACAGAAATAATAA10.8064516129032258No Hit
GTGTGCATGTTGTCCAACACCACGGCCATCGCCGAGGCCTGGGCCCGTCTGGACCACAAGTTCGATCTGATGTAC10.8064516129032258No Hit
CCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTTTCGCTTAAGTTAGTCTTACGACGGTCC10.8064516129032258No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.8064516129032258No Hit
CCTTCTCGTTTGACTTGTCCTTCTCCAACTCCTTCTCGATCTTGTCGCCCTCAGTGCCCTCCTGTTCGTTTGATT10.8064516129032258No Hit
CTCCCACTCGCTACGGAGACATTAACAAGATGGGGCCGCCTGTTCCCCCCGCCTGTATCATATACACATCTGCCG10.8064516129032258No Hit
GTGGTGGACAACACCGTGGTGGTAGAGGGCAAGCACGAGGAGCGCGAGGACGGCCATGGAATGATCCAGCGTCAC10.8064516129032258No Hit
CTGTCGTGCTGGGGATAGAGCATTGCAATTATTGCTCTTCAACGAGAATGCCTAGTAAGCGCATGTCATCAGCAT10.8064516129032258No Hit
TTATATCAGGTATGGTTCATGATAAAGTTAACAGTTAAGTTGATAACTCTTTTACTGTATATATGAAACATCTTA10.8064516129032258No Hit
TCCATGTGTAGCGGTGAAATGCGCAGAGATATGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTG10.8064516129032258No Hit
CAGCCGATCCCAAACGATGGCGCTGGCTCAGCGACCTGTCGACAATCTGTTCATCCTGTCTCTTATACACATCTG10.8064516129032258No Hit
GTGGTCGACTGGTCCATAAAGTCCATCTCGTTCTCATGGTCGCAACTGTAGCGCACTGGGCAACAGGATCCCTTG10.8064516129032258No Hit
GACTTGGCAAGTCCCAGCTTCAACACCTGCGTCTGCAGACGACGCTCCAAGAAGTCCTCAATCTTCAGACCCCGC10.8064516129032258No Hit
GTCGTAGGATGTTTTTTTTTTTTTGTTTTTTTGTTTGTTTTTATTTTTTGTTTTTTTTTTGTTTTTTTTTTTGTT10.8064516129032258No Hit
TCATTGTGCCTAGCGTTCCGTCAAATCAGCGTTTCTTAGCGTCCCAGTGGGCCCAGCCGCTGAGACACACCCCAC10.8064516129032258No Hit
CCATACTTCCCCCGGAGCCCAAAAGCTTTGGTTTCCCGGGAAGCGACTGAGCTGTCTCTTATACACATCTGACGC10.8064516129032258No Hit
CCACATGGCTGCTGGCCCGCAGCAGCCATGTGGTTGTAGACCATGTCAATGCCCACGCGCAGACCGACGCGGTGC10.8064516129032258No Hit
CTCTTTGGCCAGTTCCAGTTCAGCGTTAACTCCTGCGCCCAGAAGCCCAACAAGCACGTCTGCACCAAGATCGTG10.8064516129032258No Hit
CCTCGAGACTTCATGAGGTCTGGTGGCTCATGATGAGGTAGGAGTTAGTAGTTACTTGGCTTTTTCTCTGTGTTA10.8064516129032258No Hit
CGCTTACGTGCAGTAAGGTATTGTGCTAAGCACAAGTGTAAGGTGTAGTGGCCGGGGGAGGCGCGAGTTCGTGTG10.8064516129032258No Hit
ATAAAATTTTATTTTTAATTAATGGAAGATGAATTTGACAAATGAAAAGCATAAATTATTACTTTCGATCTTTTG10.8064516129032258No Hit
ATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATG10.8064516129032258No Hit
AGATTACTAAGAGGATCGTTCTGTTGATGAACGTTTTTGCATGCTTTTTATTTACAGTTGTCCTAGTTTTCTAAA10.8064516129032258No Hit
GGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTAC10.8064516129032258No Hit
ACAAAATTGAGAAGGTCGCTGAACGAGTACGAATAAAACCAGCAAATAAACAAATGTATCCCGCCCAGACAGGAC10.8064516129032258No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA10.8064516129032258No Hit
TGCTTTCGCTTAAGTTAGTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACT10.8064516129032258No Hit
GTCCTCGTCCGATCTTTTTAATATGATTTTTATTTAGTTAGAACACGTAGAAGAGTCTCAGGCTCACTCTCCGGA10.8064516129032258No Hit
CCATTCGTGCAGGTCGGAAATTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGTTACGGCCGCCGTTT10.8064516129032258No Hit
ATTGGGGTGTATGTTTTTAGTTATTTTTTTTTATTTTTATTTTAATTTATATTTTTATTATATTGATATTAGGGT10.8064516129032258No Hit
TATTAAAGTTGTTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTTCATACGGGTAGTACAACTTACAATTGTGG10.8064516129032258No Hit
GTTGTATTAAGATATTTTTTTATATTTTTTTTTATATTTTTTTATTTTTAATTTTATATTTATAATTATAAATAT10.8064516129032258No Hit
GCGTTCCTACAACTGGTCCGTGAAGGCCAAGAGGAGGAAGACCACCGGCACCGGTCGCATGCAGCACCTGAAGGT10.8064516129032258No Hit
TTTTTATTTTTTTATTTTTTATTTTTAATTTTATATTATTATTAAAAAATTTAAAAAAAAATAAATAGATAAAAT10.8064516129032258No Hit
CTCTAAATACAGGTATTAAAGGGGAATAACTTAGATGATCTATCTTAAACTCAAATAAACCAAACTAAGCATGCA10.8064516129032258No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA10.8064516129032258No Hit
CTCCACGCCTGCCTAATCGCTAGGGCATCGTTTCTACCCTAGCGGCGAGGTATTGGTAGTACGCTTGAGCGCCAT10.8064516129032258No Hit
TCTTAAACGGGAGACGTGTGCTCTTCCGATCTATTAAACGAGACTGAAGCTCGACACGTCTCAAAACGTATACTT10.8064516129032258Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTCCGATCAATTTTTTTTTTTTTTTTTTTTTTTATTTATAATTTTTTGACAACCAGATATGAGATGGCGATGAG10.8064516129032258No Hit
GATATAATCAGCCTTTTGCGGTTTCACTTTTAATTCCTGTGTACTTAAACATGCATGGCTTAATCTTTGAGACAA10.8064516129032258No Hit
ACCTAACAGAGAAAATACTTTAAAATTAACCTCATGCAGCCAAACTAATGTCACTGAAACCCATCTAAATCTAGC10.8064516129032258No Hit
ATATATATGTTTGTTTTAGTTGAATATTTTTTATTATAATTTATATTTTAAAAATATGAATATGCCGACGACTAC10.8064516129032258No Hit
GCGGAAGAGCCCTCTCGAGATCGGAAGAGCACACGTCTCCCTGTCTCTTATACACATCTGACGCTGCCACGACTC10.8064516129032258Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCCAGTATGGCTCGGGCAGCTTGTCCGGATACCTGTCCACGGATACGGTGTCCATTGCCTGTCTCTTATACACA10.8064516129032258No Hit
GCCGAGCTTTTTTCAGTTGATGGATTGAACTTGATTGAGGAAAAAAAGGTTATATTAGACAGAGTTTCGTCAGTG10.8064516129032258No Hit
GGCCGCGGCTGCGCGGCGCCGGTACAGTGCGTTCGTAGTAAAAACGTGTCTTATTATGTCAAGTATGGTCGCTTA10.8064516129032258No Hit
CTTCCGATCTAGGCCGGGTGCGATATTTATTTTTTATATTTTTATTATTTTTATTTTAATAATATTAGGCTGAAT10.8064516129032258No Hit
AAAAAAATATGACAAGTCTTATTATCATCCGATATTATATAATTTTTTATTTTTTTTTTATTTCAAATCATCATG10.8064516129032258No Hit
CTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACAGGCTGTCTCTTATATACATCTGACGCTGCCGACGA10.8064516129032258No Hit
CATTATATCAGTCATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATG10.8064516129032258No Hit
GTTTAAGGTGTGTTTTTGTGGGGGGGTAGTTTTACTTTGAACAAATTAGAGTGCTTAAAGGAGGTTTCAAATGAC10.8064516129032258No Hit
AATTATAATTTTTATTTTTAATTTTTTTTTATTTTTTAATTATAAATTTATTTATTAATATTTTAAAAAAAAAAA10.8064516129032258No Hit
TTTTTTTTTTTTTTTTTTTTTTTTATGTTTTTTGATTAATTGTTTTAGAAATATTATTTGTTAAATTTAATATTA10.8064516129032258No Hit
CGCTTACATACATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGACTGTCTCTTATACACA10.8064516129032258No Hit
GTTTGGGTGGTTTTGGTGGTTTTTTTATGTTTTTTTGTGTTGTGTTTGAAATTACAGGGCCTCGGATATGAGTCG10.8064516129032258No Hit
GTAATAGTACCGGCCCACAATAACACTCGTTTAAGAGCACTAATGCCTGTCTCTTATACACATCTGACGCTGACG10.8064516129032258No Hit
TAGGACTACCATCGTTCCATCGCGTAACCCGCATTCAGGGTTCGATTCCGCAGAGGGAGCCTGAGAAACGGCTAC10.8064516129032258No Hit
GATTAGGATGTGGGTCACGAATGGAGCCAGATCATGTGCCAACTCACTATACTGTCTCTTATACACATCTGACGC10.8064516129032258No Hit
TCATGATGGAGTTGTAGGTGGTCTCGTGGATGCCGCACGCCTCCATTCCCTAGAACGAGGGCTGGAACAGGGCCT10.8064516129032258No Hit
CATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACCTTTGATGGTAGTATC10.8064516129032258No Hit
GAGCTAATCAGATAAATTATTTTTTTTATTTTTTTTTTTTTTATTTACAAGTTGCAACCATGGTAGTACTAGATA10.8064516129032258No Hit
GTGCTCTTCCGATCTGATTTTTTTTTTTTTTTTTATTATTTAAGAATATTTTATTTAATTATATATTTTAAAAAA10.8064516129032258No Hit
ACCTATAATTATCTAAGAGTATTTCCCGTTTACTTATTATAAGTTATTAAAAACTTGTTTCTGACTAATATACAC10.8064516129032258No Hit
CCTTAGATGAGGTACCCGTTTCACAGGCTAAATCACCGAAATAGAACCAGTATCTTATACACATCAGACGCTGCC10.8064516129032258No Hit
TTGCCGAAGTGCTTGCAGGCGGGGCGGGCGGTACGGCTGCCCTGCAGCAGCAGCGTGTTATTGCCGGGGGGCGAG10.8064516129032258No Hit
TATCAGAACTTGTTGGGAGTTTAGTCGGTGCTAGAAATGTGTGTGTCTAAGTGTAGAGACATTGTGGTCTTACAC10.8064516129032258No Hit
GATCTGGAGGCATTGGGGCATATTTTTATGTATTTCTTAAGGGGTTCACTGCCGTGGCAAGGCCTAAAGGCTGAT10.8064516129032258No Hit
CACCGAGTCTCTCGTTGAGACAGTGCCCAAATCGTCACGCCTTTCGTGCGGGTCGGAACTTACACGACAAGGAAT10.8064516129032258No Hit
TCTAAGGAACCATAACTGATATAATGAGCCTTTTGCGGTTTCACTTTTAATTCGTGTGTACTTAGACATGCATGG10.8064516129032258No Hit
GGGTCAAATTAAGCCGCAGGCTCCACTCATGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC10.8064516129032258No Hit
CTTTAAATCCTTTGACAAGGTCCAAGTGGAGGGCAAGTCTGGTGCCCGCTGCCGCGGTAATTCCCGCTCCATGAG10.8064516129032258No Hit
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCCGGGAGTGGGTAATTTACGCGCCTGCTGCC10.8064516129032258No Hit
GTTTCGTACTATACATTTATGTATGTAAGAGTAGTACCGGTGGAGGTCTTATATGTGATTAAATAATTGTATTTT10.8064516129032258No Hit
TGAATATTCTGTGCATGGGATAATGAAATAAGCACTCTGTTCCTGTCTCTTATAAACATATGACGCTGGCGAAGA10.8064516129032258No Hit
GATCCATCTATAAGTGACAGCAAGACCGTCTTTCACTTTTGAACCATGCGGTTCAAAATCTGTCTCTTATACACA10.8064516129032258No Hit
CATCTGACCCTGCCGAAGACTCGTTACGGGTAGCTGTCGGTGGTCGGAGTATCAGAAAAAAAAAAAGGGGGGGGG10.8064516129032258No Hit
CTTCCGATCTCCCGCGGGTCGTATGCGGTATTAATTCGAGTTTCCCCCAAAAAACTGTCTCTTATACACATCTGA10.8064516129032258No Hit
TAGCCCAACAATCAAATAAATAATGGCTACCTTACGCATAGCTACGTGGCTGTCTCTTATACACATCTGACGCTG10.8064516129032258No Hit
GAGCTGTAGCGAGGGTGTGATTAGTGGTTATTTTTATAAAATTAGATAATATTTTTGAAAAAGAGGGTGATGGAG10.8064516129032258No Hit
GGTGCCAGAAGCCGCGGTAATTCAAGATAAATTATAGTATATTAAAGTTGATTAGGTTAAAACGTTAGTACTTGA10.8064516129032258No Hit
GATAAGCACGGAGCGGGCAACAAGGGATCCGGTCAGCGGCAGAACTTCATGCTGTCTCTTATACACATCTGACGC10.8064516129032258No Hit
ATATATTAATAAAATTGTTGCAACCCCAAACGGGGAATCAGGGTTCGATTCCGGATAGGGAGCATGAGAAACGGC10.8064516129032258No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATC10.8064516129032258No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG10.8064516129032258No Hit
GTGCTATTCCGATCTATTTTTTTTATTTTTTTTATATTATTTTAATTTCGCGAAATTAGGAAAGAAAATGTTACG10.8064516129032258No Hit
GAATAGACTGTTCAACCCCATGGATAGTAGAGAACGAAGATGTCTGGAGAGGGATCTTATACCCCTATTACGATT10.8064516129032258No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTAAGAACGTTTTAACCGCAACAACTTTAATATACGCTATTGGACCT10.8064516129032258No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTCATGGTAGTCCTAGGTACTACCATCAA10.8064516129032258No Hit
CTAGAATTACCACAGTTATCCAATTAACTGTTAACGATCTAAGGAACAATAACTGATATAATGCTGTCTCTTATA10.8064516129032258No Hit
GTTCGGCGATGTGCTGAGCGACAAGTAGCCGTCAATAGCTCCGCCGTCGTCCCTGCGAAAGCAGGGACCCATACC10.8064516129032258No Hit
CCCTAGTTCTAACCATAAACGATGCCAGCTAGCAATTGGGTGTAGCTACTTTTATGGCTCTCTCAGTCGCTTCCC10.8064516129032258No Hit
ACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGCTGTCTCTTAT10.8064516129032258No Hit
GTGCTATTCCGCTATTAGATATGTTATTTTTTTTTTATTTGATGCTCGTCGAGGACTGTCTCTTATAAAAAACTC10.8064516129032258No Hit
ATACAGGAGTGATTTGGGATGAGGTGTTTTTTGTTTGAGTTAAGTTTTAATAGTGTATGTTATACACATATGAAG10.8064516129032258No Hit
CCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTATGCATCGTTGCCTT10.8064516129032258No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC10.8064516129032258No Hit
AATGAAAGCCGCTTTGGCAACTGGGTTCGCTGAAGTTAGTCTTACGCCGTTCCTCGAGTGTCGCGTCTCGAGTAT10.8064516129032258No Hit
AGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACAATACGATCTGCAT10.8064516129032258No Hit
GACTAGAGCCCGGCGGGTCGGTGTTATAATTTTTTATTTTTATATTTTATTGGTTATATTTGAGGGGTAAAATGA10.8064516129032258No Hit
GGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.8064516129032258No Hit
ATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAAGGCTGTCTCTTATACACAT10.8064516129032258No Hit
GTGCTCTTCCGATGTTTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTT10.8064516129032258No Hit
CTTTCACTCCGCTCCCCGGCCGCTTAACACCTTATACTCACGCAACACGTACACAAACGCACGCCGCTATTTCGC10.8064516129032258No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTACCTCTCCGGAATCGAACCCTGATTTCCCGTTACCCGTTGCAACCA10.8064516129032258No Hit
GTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATGTGATTAAATACCTGTCTCTTATACA10.8064516129032258No Hit
GTGCTGTTGCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTGTTAATTAGTTA10.8064516129032258No Hit
ACCTTAGATGTGGTAGCCGTTTCCCAGGCTCCCTCTCCTGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACC10.8064516129032258No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers