FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73
Sequences flagged as poor quality0
Sequence length75
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGTTCTGCTTTCGGTGGGTGTCAGATCAAGAGGTAATGATTAATAGAAGCAGTTTGGGGGCTGTCTCTTAT11.36986301369863No Hit
CTGCCGGGGTTGTGTTTGGGGTTTTTGTTTTTTTGTGTGATACAGATGTGAATATGCCGACGACGCCGGGCGGGG11.36986301369863No Hit
AGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGT11.36986301369863No Hit
CCATTACACCGAAGACAATTATTGTCACAATCGTGCACGACATGCAGGTGGTGGACTCACTGCCCCCGAATCTAT11.36986301369863No Hit
GATCTATAACGATCATAATTATTTTTATTTATTTTTATTTTTAATTATATTAATAATTTATAAAATAAAAATCTG11.36986301369863No Hit
GCTCTTCCGATCTATTTTTTTATTTTTTTTTTATTTTTTATCAGCAGACACCACACCAGGAGGTGAACTTAAAAA11.36986301369863No Hit
GTGCTATTCCGATCTTTTTTTTATTTTTTTATTTTAAAGTACTCTAAAAGTTTGGTTAATTAATAGATTAAATGG11.36986301369863No Hit
GTACTAGTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGGCTGAATATTCTGTGCATGGGATAATG11.36986301369863No Hit
GGTTTTAGCCTAATAAAAGCACATGTCCCATAAGGTTCATGTTTTAATTGCATGTATTAGCTCTAGAATTACCAA11.36986301369863No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTATTTTAGTAAAAAAAAAAAAACATAAAATATAAAAGTATATCGACC11.36986301369863No Hit
GTGGGGGGGGGGGGGTGGGAGGGGGTGGGGGGAGGGGGCGTGACGTCGGGGGGGGGCGGGGGGGGGGGTGGGGGG11.36986301369863No Hit
ATCTTTATATTTTTATTTTTTTTTTACAGAAGGGAAAACGATAAGCCATTAAAGAAGGTCGGAAGTGCCCCGAAA11.36986301369863No Hit
CTAGACAGACTGCCTGCGCAAGCAGTGAGGAAGGCGGGGACGACGTCAGGTCATCATGGCCCTTACGCCTAGGGC11.36986301369863No Hit
CTTCCGATCTGGCCAGGGACGTAATCAATGCGAGTTAATGACTCACAATTACTGGGAATTCCAAGTTCATGTGAA11.36986301369863No Hit
AGTGTACAGTAGCGACAAAAGGAGGATCAGCAGGAAAAAATGGGTAAAGGTCAGAGCATGCGTGCACTCGCGATC11.36986301369863No Hit
CTTTTGTTTTTTTTTTTTATTTTTTTTTAATTGAAGGAAGACCACCTACAGTAGTACAGCCTGCGGCTTAATAGC11.36986301369863No Hit
GCATGTATGTGCCCCAACAGCATCAGGGCAATCCAAACCAAAAACCAAATGGCCTGTCTCTTATACACATCTGAC11.36986301369863No Hit
GTGCTCTTCCGATCAATATATTATTTTTTTTTTTTTTTTTTTTTTATTTTTGTATTTATTATTTTAAGAGACAGT11.36986301369863No Hit
GGTCTGGGCTGTTTCCCTTTCGAACACGGACCTTATCACCCATGTTCTGACTCCCAAGTTAAAATAATTGGCATT11.36986301369863No Hit
GCTCAGATGTTATTAAATTATTTTTTATTTTATTATGTGAATACTATTTAAAAACCACCATTAGAGCTCTAAAAC11.36986301369863No Hit
CTTCCGATCATTTTTTTTATTTTTTTTATTTTTTTTTATAAATAAAAGCAGACGCATATACACATATGACGATGC11.36986301369863No Hit
CTATAGGTGTGCTTAAAATCGTTGGGATTGTAATATTTAAGTTGTGTCTGTACTTGACGTTTAACAAGTTCTCAC11.36986301369863No Hit
CATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGG11.36986301369863No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT11.36986301369863No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTA11.36986301369863No Hit
GAAAATAAGTGGGTATGACAGCTGGATATCTCTGTCTCGAATCTTATGGTGGTGGGGCATGGCCGTTCTTAGGTC11.36986301369863No Hit
GTGATGTACGGAGCTGTTTTTTTTTTTGTTGTTAGAGTTTTATGTTGTTTTTATTATATGTATGTTTTTATTATT11.36986301369863No Hit
ATATCCTCCGGCTGATCGCCCACCGGGACCTTGGTGATGCCGGGCAGATCCACCAGCGTTAGGTTGCCCACGTGC11.36986301369863No Hit
GAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGAGCTGTTATTG11.36986301369863No Hit
CTTCAGCTAAATTTTTATTTATTTTTATTATATTTTTTTTTAATAAATGAACTTTGACCTGGTTAGCAACAAGCC11.36986301369863No Hit
GTTCTCTTAAGCTCTGTATTATTATATTTTAATTATTTAGTTAAGCTTGATCTCCCCCAGCTAAGTGGTGGACAC11.36986301369863No Hit
ATTAAGATATAATTTTTTTTATATAATAATTTTTTTATATTTATTTATTTTATTTTTTTTTATTTAAAAAATTTA11.36986301369863No Hit
TCCCATAAGGTTCATGTTTTAATTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAACTGTTAACGAT11.36986301369863No Hit
AAAAAAATATTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.36986301369863No Hit
GTCGTCGCGGCCAGCGCGGTGCAGGTGACGATCAAGGGCCTGGACGGCAAGGACCAGGCCCTGGCCGCGACGACC11.36986301369863No Hit
GTTTTTGTGGTTTTTTTTTGGGTTGATGATTTATGTGGTGTTTTTATAAGTGATGTTGGAGCGCTGGAAACACAT11.36986301369863No Hit
GGGGGGGGGGGGCGGGGGGGCGGTGGGGGGGGGGGGGGGGGGGGGGGTGGTGGGGGGGGGGGGGGGGGGGGGGGG11.36986301369863No Hit
CTTCAGCGCAAGAATATTGTATTTATTTTAATATTTTTTTTCGCCACCACCCCAAAATCAATCGGTGAGGCAGCA11.36986301369863No Hit
CACCCGGAAAGGCGTAGATCCGCGAACGCATCCGGACTGCTGAGGCGAATAGATACCTGGCGATCTGTTTCTGGC11.36986301369863No Hit
CTGATATAATGAGCCTTTTGCGGTTTCACTTTTAATTCGTGTGTACTTAGACATGCATGGCTTAATCTTTGAGAC11.36986301369863No Hit
GTGCTCTACGTTGCATTTTTTATTTTTTTTTGTAGGCCGACCATGTTGGCAAGGGGTATATGGCAATCAGGGGTG11.36986301369863No Hit
CGTCTCCCCTTCCAGATCGGAAGAGCACACGTCTCCCCCTGTCTCTTATACACATCTGCCGCTGCCGACGATATG11.36986301369863Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTAAGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATACAGCTAAATAGAATAGATAAGAACAGTGGAATAA11.36986301369863No Hit
GCCCAAAAGCTTTGGTTTCCCGGGAAGCGACTGAGAGAGCCATAAAGGTAGCTACACCCAATTGCTAGCTGGCAT11.36986301369863No Hit
GTGCTCTTCCGATCTTGTTTTTTTTTTTTTTTGGTTTAGTAGTACTTATGACACTTATACCCATCGGACGATGAC11.36986301369863No Hit
GGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGGTCTTGATTAATGAAAACATCTTAGGC11.36986301369863No Hit
ACTCTGAACTCTGACCTCTGAATGTACTCACCCGATCAGCTTAGCTACCTTTTTCTGGAGCCGCGCCGCTCAAGC11.36986301369863No Hit
CTTTTCATATGTTCCTCCTATTTAAAAACCTGCATTAGTGCTCTTAAACGAGTGTTATTGTGGGCCGGTACTTTT11.36986301369863No Hit
GTGCTCTTCCGATCTTTGTTTTTTTTTTTTTTTTTATATTTTTTGAAAAGTTTTTAAAGGAGATGATGTTGTCAT11.36986301369863No Hit
GTTTCGAACTTTTTTATTTAATTTTTTTTTATTTTTTTATTAATGAAAACAAATTTGTAAAATAAAATCTCTTAA11.36986301369863No Hit
ACGAAGGCTCTTGAACTTTGGATACTCCAATCGATTGACATTCGCTTGGGTTCACCAGGTTGCAAGGCAATTAAC11.36986301369863No Hit
TGCATGGCTTAATCTTTGAGACAAGCATATACCTACTGGCAGGATCAACCAGCATCCCTGGTTAGATCGTATGCT11.36986301369863No Hit
GGGCCGAGGGTTGGTGGGTTGTTTTGGGGGGGGGGAGTGGGGGGGGGTGTGGTGGTGGCGGTGGTTGTTGTTGGT11.36986301369863No Hit
GTGCTATTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTAAGAATGTAAAAGAATTTCACCTGTCGC11.36986301369863No Hit
GTTGCGGGCTGGGTTGGGAGCCGTGTGGTCTTCCGAGCTCTTTAGGGACTGTCTCTTATACACCTCTGACGCTGC11.36986301369863No Hit
GTCATCGTCAGCTCTTTGTATATTTTTTTATAATTTTAAATTTGCAAAAAAGGAACACCTAGATACTAAAATAAA11.36986301369863No Hit
CATCGGATCTTGCCAGGGTACGTGTGCGCTTACGTGCTTTTTTTAGATTTTTTATTTTTTATTTTTTTTTTGTGT11.36986301369863No Hit
GGACGGAGTTGTCCTGCACTTTGACAACCAGTTAGCTGGGCTTGAAGTGCGCCACATCAATGCAGACCTGGAAGC11.36986301369863No Hit
GTGCTATGGGGATCTTACGCTGGAGACGTGTGGTATGGCGAGCGATATTTTTGTTTGTTTTTTTGAAGTTTGATT11.36986301369863No Hit
GGTAACAGGGAGTCAGCGGACGCTTCCATTGCCCGAGCCAGGGTAGGGGCGGGAGGAACTAAGGAAGGCAGAACG11.36986301369863No Hit
GTGCTCTGCCGATCTTGTTTTGTTTTTTTGTTTTTTTATAATGTTTTTAATTATTTTTTTTTTGTTTTTGAAGAG11.36986301369863No Hit
TCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTA11.36986301369863No Hit
GTGCGCTTAAGAGCTTGTATGTTTTTTTTGTAAAGTATATTACCATCGCGATGATAGTGGCATTCGTGATGCGCG11.36986301369863No Hit
GGGTGGGGTGGGGGGGGGGGGGGGGGGCGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGG11.36986301369863No Hit
AGCTCGGATACATCGCCCCGCCCAACCGGAAGCTGCCCCCCGTTCCCGGATCCAACTATAATACCTGTGACCGGA11.36986301369863No Hit
CTAAAAATCTTTATATTAATTTTTTATTTATAATTTAAAAAAAATTCACCACTCTAGAAGTAATAACTGTAACAA11.36986301369863No Hit
AACGAGACCGAGTCGGTGGTCACCTACCTGTCCGTAAGCAACGCCAAGCTGCAGGACATCGAGAAGCTCACCGTC11.36986301369863No Hit
CCATTCATTCCAGCCTTCAATTAAAAGACTAATGATTATGCTACCTTTGCACAGTCAAAATACTGTCTCTTATAC11.36986301369863No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTAATTTATTTTTAAAGCAGAAGAGTTAAAAAAATATACAGTAG11.36986301369863No Hit
CTTCAGATCATTTATTTTTTTTTTTTTTTTTTTATTAAAAAGAAGATAAAGTATAAAAATAAGAGATTGAGAAAT11.36986301369863No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGGAAAGGCAGGTGGAAGAATAAAAAATAATACCAGGGCCAAAAT11.36986301369863No Hit
GTGCTCTGCGGAGCTGGTTTGTGGGGTTTTTGTTATTGTTTATTTTATTATATTTATGTTTGTTTTGTAGATTTT11.36986301369863No Hit
GTGCTCTTCAGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATTTTTTATTTTTTTAAGAATTTTGTTAAGG11.36986301369863No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAC50.069.065
CGCGACG50.069.066
GGTGACG50.069.024
TCAAGGG50.069.032
GTGCAGG50.069.019
GCAAGGA50.069.047
CCAGCGC50.069.011
GCGACGA50.069.067
GTCGCGG50.069.04
AGGACCA50.069.050
GTCGTCG50.069.01
GCAGGTG50.069.021
GGACCAG50.069.051
GGCAAGG50.069.046
GGCCGCG50.069.063
TCGCGGC50.069.05
GCGGTGC50.069.016
GCGGCCA50.069.07
GACGGCA50.069.043
AGGGCCT50.069.035