FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length75
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAGGACCGCCGTAATGATTAATACGGACAGTCATGAGAAACACAAAAAAATTGTCAGAAGTCAAAATCCTGT11.3157894736842104No Hit
AGTCTAATACTAGGCCGGTACTATTACTTAGAACAAATTAGACTCCTTAAAGCATGTCACAAATACACATCACCC11.3157894736842104No Hit
CGTGTGCTCTTCCGATCTGAACAGGGAGACGTGTGCTCTTCCGATCTGAGACGGGGTGCTCCTGTCTCTTATACA11.3157894736842104Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATTTCATTTGTTTTCAGATCAATAGGTAATTATTAATAGAATCCTGTCTCTTATACACATCTGACGCAGCCGAC11.3157894736842104No Hit
AATTTACGCGACTTCAGCCATCATTAGATATGGTAGACTATTCTCAGACTCCCTCAACGGCTGTCTCATATACAA11.3157894736842104No Hit
AATCCGATCTTTTTATATATTTTTTATTTTTTTTTATTATTGAATAAAAAACATGTTAAAGATCAAGAGTATTAT11.3157894736842104No Hit
ATTCCAATCTTTTATTTTTGTTAGTATTTATTTTTGAATGTTTATTTTTAAAATATTTTGTTAAAGTATAAAAAT11.3157894736842104No Hit
CTTCCGATATTCCCATCCACATACCATCAAATTAAAAATAATTAAAATTAAAATAAAAAAAAATAAAAAAAATAA11.3157894736842104No Hit
ACATACTACCAACAAAAATTCATAGTGCAAACATTAGAAAGATCTGTCTTAGGTACAATAACATACAAACTGAAT11.3157894736842104No Hit
CCAATGAAGCACAAGTTCAACTACGCACGTTTTAACCGCAACAACTTAATATACGCTCTGTCTCTTATACACATC11.3157894736842104No Hit
ACCAAATATAAAAAAGGATCAAGTATACCACTTAAACCACTGATAAAAGGTGACACAAATAATAATTTAAACAAA11.3157894736842104No Hit
CTTTAATGTTTATGTTTTTTATGAGCGCAAAACAAATGACTAAAATTAAATAAAATGAAGAGAATAAGTGAAATA11.3157894736842104No Hit
GTCCTATTAAGATCTATTTATTTTTATTTTTTTTTTTTTTTTTTTTTTTTATATTTTTAGATTTTATAATGATAA11.3157894736842104No Hit
GTGCTGTTAATATATTTGTTTTTATTTATTTTTTTTATTTAAAATTATGGGAAATTTTATTTTATTAGGCTAAAA11.3157894736842104No Hit
GAACAGAGGTCTTATTTAATAATCCCATGCACAGAATATTCAGTCATTTGAAGCCTCCTTTAAGAACTCTAATTT11.3157894736842104No Hit
GCCCAAGCTCTATGCGAAGCTCCACTACTGCGTGTCCTGCGCAATCCACTCCAAGGTGGTGCGCAACCGTTCGCG11.3157894736842104No Hit
GAGTCACACTGGGTTTCCCCATTCGGGTATCGCCGGTTGTTGCGGTTCATATCACCTTACCGGCGCTTATCGCAG11.3157894736842104No Hit
GTTTCGATGTTTTGTTGTTTTTTTTTTTTGTTTGTGTTTGTATTGTTGAATTATGACGATGGGGGGGGGTTAGAG11.3157894736842104No Hit
GATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTACTGTATTGTTATTTTTCGTTACTACCTCC11.3157894736842104No Hit
TTTTTATTTATTTTTTTTTTTTTATTTTTTTTATAGAGAAAATTTAATAAAAAAGGAATGGGCATGATTGGAGTA11.3157894736842104No Hit
ATGTTATTCTATGTTATTCTGTGATATTCTATGTTATTCTGTGATATACTATGTCTGTCTCTTATACACATCTGA11.3157894736842104No Hit
CATACGATCTTAGTATAATATTTTTATATATTTTAAAATAAAGTGGTGCACAGTTAGGATTATACAATGCAAATC11.3157894736842104No Hit
TTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCA11.3157894736842104No Hit
TTTCTAACCATAAAAGATGCCACCTAGAAAATTTGTGTACATACAAATATGTCTCTAACAGACTCTTCACGAGAA11.3157894736842104No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTTCTCATCGAGCTCAC11.3157894736842104No Hit
GACACTAACCACAGTATATATGAATTACAAAATAAACACCACCATTCTCAATTGAGGTCGCCACGTAATCGGGCA11.3157894736842104No Hit
CGGTATGATGATGTTGTTATGGTTGTTGCATGTTCTTATTGTCTGTCTGATTTTTTCAACCCAATATCATAATGT11.3157894736842104No Hit
CTTCCGAACTGTTTTAATTATATAAAAAATAAATTAAATTAAATAATTAAAATTATTATAAAATTTGTTTTATTT11.3157894736842104No Hit
TTCAAAAACCGAGCTAAAAATATAAGATTGAAATTTATATGCGCTGCTGAGGATTAATAATGAGCAGATCTGTTG11.3157894736842104No Hit
TACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAACCCAATGAAAGCAGAACAGAGGTCT11.3157894736842104No Hit
CGACGGCACTGTTTTTTAATTAATTTGTATGTTTGTTTATTGAGTGTTTTTTTTTTTTTTTAATAGTATATTTTG11.3157894736842104No Hit
ATAAAGGGGATAGGTGGAAATTAATATAAATAATTATTATAAATAAATGTTGTAATCATAAGGAAATGAGACCAC11.3157894736842104No Hit
AATAAACACCGACAGCTAACCGTACACACTAAGAAACCGTTAACACTCAACATGCAGATTATCTCAGTTCTGTAT11.3157894736842104No Hit
AAGTCAGACACTTACAAGAGGAACGATTATGTTTTACGTTTAGAAGTTCTATTAATTTTGATATTATTAATAGTA11.3157894736842104No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAACTGTTAACGATCTAAGGAACCATAACTGCTGTCTCTTAT11.3157894736842104No Hit
ATACCGATCTTTTTTTTTTTTATTTTTTTTATTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTAATTATAAATA11.3157894736842104No Hit
GGATAATGCAATTATTATTTTTAATATTTTACAAAGAGGATGGTAATTGTAAGTCTATTATAAACATAAGACGCT11.3157894736842104No Hit
AAAATTATTCGATAATATAACCTGTATCAAACTCTTCAAGATAACATTGTACCCACTCACAATAAAAAAAACACA11.3157894736842104No Hit
GTCCTGGTCCGCGCTGTTGTTGGGTTGGTTTTATTTTTTGTTGGTATTGTTATTGGGGTTGGCAGTGACGTTGTC11.3157894736842104No Hit
TAAACCACCTATTCAAGAAGACTAACCACCAACGTGACCACTTTCTCTTATAAAAATCAGACACTGACGACGAAT11.3157894736842104No Hit
TAATAAATGATCCACAAGAAATTGACATAGTCAGTCTTAAAACAATAATCTAAATCTCTAATAAACAAAACAGAC11.3157894736842104No Hit
AGCAAATACAAGCAATTAAAACATGAACATATAGCGACATGTCCTTTTATTAGGCTAAAACCTCTCACATATACA11.3157894736842104No Hit
GTGCTCTTCCGAGCATTTTTTATTAAATATTTGTTTTTGGTGTAGCTAAAAAAAAGGATCACGCAGACACACTCT11.3157894736842104No Hit
TCAATGACTATCTCAAGATCAATATGAAATCAAGACTAATCAACAAACATGCTTATTTGACGGATTCTATTGCCT11.3157894736842104No Hit
ATTTAAAAAAATCAATTAGTGCTATTAACATATTGTTATTGATCTATATTATACAAATCTCACACAGAACACAAA11.3157894736842104No Hit
ACCAAATTGAATTAGATACTTAAATGTCTCTCACAGACGCAACACGGTCATACAAATCATTTGGTCACCCGTGGA11.3157894736842104No Hit
GTTTAGAAATAGGGAGGTATCTGATCGACTGCGAAACTCTAAATTACGTTCTGGATTAATGAAAACATATTTGGA11.3157894736842104No Hit
TTTCCAATGAGAAGAGTTTTCATATAAAACACCCAGAGTCCATCATCATAACAATACATTACATACACTTCTCTA11.3157894736842104No Hit
CTACCAAAGTTGCAAAGGGTAACGTTCAATAAAACATACATTCCGTAGCTCACACAAAAACACATCTGAATCTGC11.3157894736842104No Hit
GATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTTTCGCTTAAGTTAGT11.3157894736842104No Hit
ATAAACAACATTATTAATTTTATATTTATTAAATTTGAAACATCAGACCAAAATAAAAGACAAAAAAAACAACAA11.3157894736842104No Hit
GTTATGGTAAAATAGATCGATAACAGTTACTTGGATAACTATGGTAATTCTAGAGCTAATACATACAAATAAAAA11.3157894736842104No Hit
GTGCTATGCCGATCTTTTTGTTTTTTTTTTTTTTTTTTTGGTGGTGGGGGAAAAGGTGTGGAGGGGGAGGGGGGG11.3157894736842104No Hit
CTTCAGATCTTTTTTTTTTTTTTTTTTTTTTTTTTATATATAATTATTAAACAGAAAGACGTCAGGCAGGTCGTG11.3157894736842104No Hit
ATTAGGAGCATGTTGGTTTTATAAATTTTTTTTTTAATTTTTTTGATAATAATTAAAAAATATTTTTAAGTTAAA11.3157894736842104No Hit
TAATAAACCCTAAAATTCACATATCAATAAACACGCAACAACACTTCAACAAACACTTTCTCCTCTATTCACCAT11.3157894736842104No Hit
CTTAAGATAAATTTTTTTTTTTTATTTTTTTTTGTTTTATGTGGTAATTATAGAGATAAAAAATGAAAGTAAACC11.3157894736842104No Hit
CTGTTGGTGAATCATGATACCTTCTCGATCGCATGGCCTCGTGCTGGCGATACTTCATTCAAATTTCTGCCCTAA11.3157894736842104No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTATTTTTTATTTTTTTTT11.3157894736842104No Hit
TTTTTATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTTTGTTTATGTATGTTTTTTTGTTTAGTT11.3157894736842104No Hit
GTTAAATAGAAATTTAACACTTAATTGAATAAAAGTATTAATTCTAAAGATAATACCAGTCTCAAAAAAACAACA11.3157894736842104No Hit
GTGGTCGTGTGATCTTGGGTTGTTGTGTTGTTTTGTGTGTTGTTGTGATGTTGTGGTGTGGTGTTTGTTGTTGGT11.3157894736842104No Hit
ACTCGGCGCCGATATCGCACACAATAACTACAAGGCCGGACCCATTTTGACGCGCGCAGCTCTCAACCTGTCTCT11.3157894736842104No Hit
CTCAAAAAGATTAAATTAAAGTTCATGACGTTAAAACAATCTAAGTTTAACTACTGCAAAATACGCGAAATACAA11.3157894736842104No Hit
CTTCGGATCGTTTTTTTGTTTTTTTTTTTTTTGATATCAGGCGGGGCAGTATCAGTAAAATAAAAAAGGGTTTTT11.3157894736842104No Hit
CGAACATTCATTATATATTTATATTTTTTTTGTATTTTTATGTAGTAGAAACCAATCGACTGGTACATCGTGTCG11.3157894736842104No Hit
GCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATAACCTGTCTCTTATAC11.3157894736842104No Hit
GTGTGGAAGAGAAGGGAGTGGGGCGAAATCACGCATACAACATAAAATAAGGCAGAATTAGTGTTTCATTGGGGA11.3157894736842104No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTCATCTGGAAAAAAAAGATACCAGAACAG11.3157894736842104No Hit
CTAACAAACTTATTTTTAATTTTTTTTTATTATTATATAAAAAAAAAAAAAAAAAATAATAATAAAGAATTAACT11.3157894736842104No Hit
TTTATTTTTTTTTTATTATTTTTTACAGAACACAAATTGGAAATTAAAAAAAAAAAGCAAACCAAAAAAAGTTAA11.3157894736842104No Hit
AATAAATGAAATTAAAACATGAACCTTATGTGAAATGTGCTATTATTAGGCTAAAACAAAGAGATAGAAAGATCG11.3157894736842104No Hit
CAGTTATACAAGAAACAGTTAACCAACTAATCAAACATAACTGATATAATGAGCCTTTTGCGGTTTCACTTTTAA11.3157894736842104No Hit
CAAATCACTCACCCCAAGCAATACACCCTCTAGAAAACCAAGTTGTCCAAAAACTTCATACCCATCTCCACCCGG11.3157894736842104No Hit
GCAAAGAACAATTATAATAATATTAATTATTTTTATTCCTGCTTTAAGCACGCTAATTTGTTCAAAGTAATAGTA11.3157894736842104No Hit
TAGATCTTCCGATCTAATTTTTAATAATTTTTAAATTTTTTTATATTGTGTAGGAGTGAAGTGTAATAGGATAAC11.3157894736842104No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAA50.069.034
TAGAAAG50.069.035
ACAAACT50.069.065
ACAAAAA50.069.012
AAACTGA50.069.067
TTCATAG50.069.019
CCAACAA50.069.09
AACAAAA50.069.011
GTGCAAA50.069.025
TAGTGCA50.069.023
AAACATT50.069.029
AAATTCA50.069.016
TGTCTTA50.069.045
ACTGAAT50.069.069
AATTCAT50.069.017
ATTAGAA50.069.033
AACTGAA50.069.068
TGCAAAC50.069.026
ACCAACA50.069.08
CTTAGGT50.069.048