FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length75
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAATCCTGTCTCTTATACACATCTGACGCTGCCGAC22.5316455696202533No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGACGTCTAAGGCTGTCTCTTATACA11.2658227848101267No Hit
CTTTAGATCATTTTGTTTTTTTTTTTATTTTTTTTATATATTGAGGCACAGATATAAGTGGAGCTTTGAAATAAA11.2658227848101267No Hit
CTTTACGCCCAGTGATTCCGAGCAACGCTAGCCCCCTTCGTATTACCGCGGCTGCTGGCACGAAGTTAGCCGGGG11.2658227848101267No Hit
CAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATTG11.2658227848101267No Hit
CTTCCGATCTACTTCGGGAGACGTGTGCTCTTCCGATCCTTTTTTCTGTCTCTTATACACATCTGACGCTGCCGA11.2658227848101267TruSeq Adapter, Index 3 (95% over 21bp)
TTTCTATGCTTTTCCGCTCAGCCAGCTGCACTTGGATGTTGTGGGGGATCGGGTGGTGGTGCTTTACAACTTGTC11.2658227848101267No Hit
ACCCAATTGCTAGCTGGCATCGTTTATGGTTAGAACTAGGGCGGCTGTCTCTTATACACATCTGACGCTGCCGAC11.2658227848101267No Hit
ATCTTTTTTATATTTATTTTAATATTATTATTTAAGAATAATTTAAATAAATTTTTTAATTTTTTATTTATTTAA11.2658227848101267No Hit
GTGCTATTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT11.2658227848101267No Hit
GCACTAACCATCTAATATTTTATTTTTTTTTTTTTTTAATAATAAAAACGAACACCAAAGAGTATATCTAGGAGT11.2658227848101267No Hit
GCCAGAGAGTTATGAAACAATAAAAAAGCGAATCATAATATCTGTCTCTTATACACATCTGACGCTGCCGACGAA11.2658227848101267No Hit
GTGCTGTTCAGATCGTTTTATTTATTTTTTTTTTTTAAAGCATATACATGCAATTAAAACAAGAACATTATGGGC11.2658227848101267No Hit
TGTGAAAACTGGTTTGCAAAATTACTTTAGGGCCACCATTTTAGGGCTACCCAGTCGGCGTTTTTCGTTTCGGTG11.2658227848101267No Hit
TGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTAT11.2658227848101267No Hit
CTACAGAACTTTTTATTTTTATTTATATTTTTTTTTTTTTTTTTATTTGAGAAATAAACAAAAAGATGGTAGCTT11.2658227848101267No Hit
GAGCTATTCCGAACTTTATATTTTTTTTTTTTTTATTTTTTATTTTTTAATAATATTAATATTAAGAAGAAGAGG11.2658227848101267No Hit
ACCCCCAAGGCTTCGTCACGAGCCTCCACGCCTGCCTACTCGCCGGGGCATAAATCCTGCCCCGGCGGCGGGGTA11.2658227848101267No Hit
GGATACGGATTCGGATTGAGATTTAGTTGTGGGTAAGCAAGCTGAGGGGCGGCTGCTGGCTGACGGGAGCCGCTC11.2658227848101267No Hit
TTTCATGGGATATGGCGACATTCGTCTGGCCAAGCGCTTGGACAACAATCATGAAGTCGCTGGTCAACTGCGCGG11.2658227848101267No Hit
CATCCGATCTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTAATAAAAAAAAAAAGA11.2658227848101267No Hit
CTGCAGCTCTTTTTGGTATGTAGTGTTTTTAATTTTGCCCACAAACACCATCGACTGTATATTAAACACATCTGA11.2658227848101267No Hit
CACTTCCGAACTTACCAGTGATTCTCGTTGAACCTCCCAGTAGTTATATGCTTGACTCAAAGATAACGCCATCCA11.2658227848101267No Hit
GTTGAGATCTTTTTTTTTTTTATTTATTTTTATTTTTATTTGTTTTATTTTGTTATTATAGAGATAATAAATGAA11.2658227848101267No Hit
GGCCCCAATCTTTTGCGCCGCCCCACCAGCATCTTCTTCTACTTCTATACTGCCAATCGCATTTTCTTCACCTAT11.2658227848101267No Hit
ATACAAATCAGCATAAAACTAAAAATAAATCAATAAAAAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.2658227848101267No Hit
GTGCGGGTGCGATCGTATTAATTTTATTTTTTTTTTTTTATTATATTATTATATTAATTCTTATTGTGGATGTAC11.2658227848101267No Hit
ACCTACGTCTATGCCATGTGCCTTGTTTTGGCCACCAATTTGTTAACAGTGAATGCCATTCGGTGCCATCAGTGC11.2658227848101267No Hit
ACATTATTCTATCGACTAGAGACTCTTCACCTTGGAGACCAGCTGCGGATATTGGTACGGCCTGTTGAGAAGTTT11.2658227848101267No Hit
GAACCCATCTTCGCCCTCACCTACCTTCGCCACGCCGGGGGACAGGTGTTTTTGGCGGAACATCCCCTCTTGAAG11.2658227848101267No Hit
ATTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATTTTATTATTTATTATTTTTATT11.2658227848101267No Hit
ATTTTGGCTAGAGAACGTGCTCAGCGTGGGGGCTTGGGCTAGCATGTCTCTCGAGCTGCGCTGCCGCAGGCGCGC11.2658227848101267No Hit
GTGCACTACCGAACTATTTTTTTTTTTTTTTATTAATTTTTTTATATATTTATATAGAATTATAAAAAAAAAATA11.2658227848101267No Hit
GATTAGCTTGACCCTGGAACCCTTAGTCATCCGGCGGACGGGTTTCTCACCCGTCATTTGCTACTCATGCCTGCA11.2658227848101267No Hit
CATTTAGCGATCTTTACTGTCCCCGGATACACTAAGTAATAACGCAGGATAACATACAAGTTAAGGTACAAAGAA11.2658227848101267No Hit
CTTCCGATCTGGTGCGGGGTGCTCTTCCGAAACTGTCTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGT11.2658227848101267No Hit
ACCCCGGGCCCCCGGACCGCCTCGGCGGGCCGGGTGAGGAGGGGCTTGATGACGGCGAAGGCTGTCTCTTATACA11.2658227848101267No Hit
CTTCCGATCTAGGCCGGGAGACGTGTGCTCTTCCGATCCTGTCTCTTATACACATCTGACGCTGCCGACGAAGGC11.2658227848101267TruSeq Adapter, Index 3 (95% over 21bp)
ACGGTATGGAATATGTCCGCGCGGCGCTGGCCAAGGGCCTGAACCTGTCTCTTATACACATCTGACGCTGCCGAC11.2658227848101267No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACTTTATCTATTACGCAACTTAAGCTCAAGACAAACGGTTAGGATTACAA11.2658227848101267No Hit
GTATTGGGATCATTCCGGCGCAGTTCCCACCACACGGGCAGCAGGAAGGCCGCGAAACCCAGGAGCAGGCCGGTC11.2658227848101267No Hit
CCTTAGTGTATTTTATAAACATTTTCTCCATAATATTGGTTTCTCCTTTAATTTCTCCTTTGTTAAAGCACTAAA11.2658227848101267No Hit
TAGCTCAACAGAAAATAATTTTTTTTTTATTTTTTTTTTTATTAATATTTTAATATAAAAATAAAACAGAATACT11.2658227848101267No Hit
CCCCCGAAGGAGTGCATCCGAAGCTTCGGCTCGTGGTTTGAGCCCCGTTACATTTTCGGCGCAGGACCCCTTATT11.2658227848101267No Hit
CTTACGATCTTATTTTTTTTATTTTTTTTTTATTTTTTTTTTATATTTTATTTTTATTAAAAAAATTAAAAAAAA11.2658227848101267No Hit
AAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAACTTTAATATACGCTATTG11.2658227848101267No Hit
CCTCTGCCCTGGTTGCCCAAGACAGAGGCCTCCTTATCCCGAAGTTACGGAGGCAAATTGCCGAGTTCCTTCAGC11.2658227848101267No Hit
ATCTCGTTGGCCATGTCAAAGGCGTCGTAATCACCGCAGTACTCGTCGTCGTTGAACAGCTGCGGAGGAAGCGGA11.2658227848101267No Hit
TCCGCAGACAGGCGCTGGCCGATGACTTTTTGGCAAACTCAACTATGCCCTCGCCCATATGCTTGCCACGATCGT11.2658227848101267No Hit
AACTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATG11.2658227848101267No Hit
AGACAAATGTGCTGTCCACCACTGAGCTGCCGCAGATCAAGCTCTACCGCCGCTGGTCTGGCGACGATCTCACCG11.2658227848101267No Hit
GGATCATGTGGGAAATGTCGGCGCTGTTCGAGAATATCGGCACGGTGCATGACGGCATCGGCTCGATTTCGCTGC11.2658227848101267No Hit
TCAATTGCTTGGGTGAGCTGGGATTGCGGATCGGTGTCGGTCCTCTCTCTTATACACATCTGACGCTGCAGACGA11.2658227848101267No Hit
CCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTACCCGCAACAACTTAAATA11.2658227848101267No Hit
GTGCTAGTCCTATCGTTTTGTTGTTTTTGTTTTTTATTTTGTTTGAAGAAAGTGTGTATTAAACACAACTCACGC11.2658227848101267No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTGTTTTTATTTGATTGGTTTTAAGAGAAAGAGGTAAG11.2658227848101267No Hit
GGCAGGAGGATCACCTGAGTCCAGGAGTTCCAGGCTACAGTGAACTAGGATTGAGCCACTGCACTAAGACTGTCT11.2658227848101267No Hit
GAGCGACGCCGCCCGCGACGCCAACCTGCAGACGGCCGGCATCCGCACCCTGCGCTGGCACTCCACACGCAAGCC11.2658227848101267No Hit
GACATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAG11.2658227848101267No Hit
GCTTAATTTCGGTGATCGGACGAGAACCGATGTATTCAGCGTGGTATGGTCGTTGGCCTGTCTCTTATACACATC11.2658227848101267No Hit
CAAAACTTGGGATCCAGTCCGCAAACGGACCCGGAGATTCTTCCACCCGGTCCGGACTCGCTGGGAGAACATTTC11.2658227848101267No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAAC11.2658227848101267No Hit
TATTTTTTTATTTATATTATATATTTATTTAATATAAATTTTTTTATTTATTAATGAAAAAATAAATAAAAAAAA11.2658227848101267No Hit
GTGAAATTAAGATATATATTTTTTTTTATTTATTTTTTTTTATTTTTTATTTTTATTTTTTTTTTTTTTATTTTT11.2658227848101267No Hit
AAGCTTTGGTTTCCCGGGAAGCGACTGAGAGAGCCATAAAAGTAGCTACACCCAATTGCTAGCTGGCATCGTTTA11.2658227848101267No Hit
CCATTTGGGGCTGGTGATAGGAGTTAAAGTGTACTCATTCCAATTTCAGGGCCTCGGATAGGAGTCTGTCTCTTA11.2658227848101267No Hit
GCCAGGCGTTGTACAACAAGGCATTGAAGGTGCTGGCATGCCCGCGATCTGGCTGAAGATCCTTCCTTATATCTG11.2658227848101267No Hit
GTCAATAACCTCGTGAGGGTGCTTTCCGTCAACGGTGCAGCCTACGCTCTGGGCGCTTCCCAGAACTTCCTTGCA11.2658227848101267No Hit
CGTTTTAACCGCAACAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCT11.2658227848101267No Hit
ATTATTATTTTTATTTTTTTTTTTTTTTTTTTTAATTTTTTTTAATTTTATTTTTTTTTTTTTAATATATATATT11.2658227848101267No Hit
CCATAGTGAAAACTGCAGAGATCACATTCCTGCGCGGCCCCGTCAAGAAGCCCATACCCGATTACATTCTTCGCG11.2658227848101267No Hit
GATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTT11.2658227848101267No Hit
GATAGGAACAGCCTACATCGCATCCTCGCACTGCGCCGACGCGATGACCCCCGGGGCTCCTGTGGCTAAGACACG11.2658227848101267No Hit
ACCATACTTCCCCCGGAGCCCAAAAGCTTTGGTTTCCCGGGAAGCGACTGAGAGAGCCATAAAAGTAGCTACACC11.2658227848101267No Hit
AACCATAAACGATGCCAGCTAGCAATTGGGTGTAGCTACTTTTATGGCTCTCTCAGTCGCTTCCCGGGAAACCAA11.2658227848101267No Hit
ACTACGGACAAGTAAAGGTCGATGCTTAAAAAGTGGCAAACACTAATTGAAGCTATTGTCGAAGCAAAGACTGTA11.2658227848101267No Hit
GAGCACACGTCTCCCTAGTAAGATCGGAAGCTCACTCTTCTACAAAGATAGATCTGCAGAGCAAGCCTATCCAGA11.2658227848101267No Hit
CCTTTGCACAGTCAATATAGTGCGGGGTTTTAATAGTTTCAGGGGGGAGGTTTGACTGTTTTTTGAAGTCAAAGT11.2658227848101267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT50.069.040
TTGTTAT50.069.050
GTTGAGA50.069.01
ATCTTTT50.069.07
TAAATGA50.069.068
TTATTTG50.069.036
TTATTTA50.069.020
TTTTTTA50.069.016
TGTTTTA50.069.041
GATAATA50.069.063
TTATTAT50.069.053
GTTTTAT50.069.042
TGTTATT50.069.051
TTATAGA50.069.056
TTGAGAT50.069.02
ATAATAA50.069.064
ATTATAG50.069.055
ATAAATG50.069.067
GATCTTT50.069.06
TATTTTG50.069.046
Produced by FastQC (version 0.11.2)