FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n02_zt14wtlarvs13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n02_zt14wtlarvs13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4423568
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG225320.5093625779009162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA23200.033.0380021
TTCGGAA25200.030.2724232
CTTGTGT80600.026.49210469
CGATCTG247850.026.101565
CGATCTA123750.024.1170965
CCGATCT634950.023.7422124
TTCCGAT658500.023.0701852
GATCTGG95900.022.916876
TCCGATC676300.022.54443
CTTCCGA707750.021.5816211
CCTTGTG99750.020.99116768
ATCTGTG20150.020.199857
TCTGAGC15200.020.1979488
GATCTGA73500.020.0905066
TGTGTAG41150.019.86732169
GATCTAG46250.019.8428636
ATCTGAG25900.019.3112077
ATCTGGC38000.019.2439167
GATCTGC103950.018.9516096
ATCTGGT32100.018.9124937
Produced by FastQC (version 0.11.2)