H27VGAFXY_n01_zt14wtlarvs83.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H27VGAFXY_n01_zt14wtlarvs83.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5215985
Sequences flagged as poor quality	0
Sequence length	75
%GC	52

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	25930	0.49712566274634606	RNA PCR Primer, Index 29 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20046	0.3843185898732454	No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG	11378	0.21813713037901758	RNA PCR Primer, Index 29 (96% over 30bp)

Adapter graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCTAATG	2265	39.60601	69
TTTCGGA	4120	34.254322	1
TTCGGAA	4475	31.841093	2
GTCTTCT	7595	30.484848	49
GTATGCC	7810	29.860243	42
GCCGTCT	7835	29.639124	46
TATGCCG	7885	29.619972	43
CCGTCTT	7900	29.264223	47
TGCTTGA	7945	29.048914	55
ATGCCGT	8020	29.03535	44
GCTTGAA	8145	28.801527	56
TCGTATG	7955	28.795563	40
CGTATGC	8180	28.340902	41
TCTGCTT	8215	28.096056	53
CTGCTTG	8260	27.98476	54
CTTGAAA	8335	27.855255	57
TGCCGTC	8440	27.678051	45
TTCTGCT	8340	27.592218	52
CTTCTGC	8480	27.384731	51
TCTTCTG	8460	27.32711	50