FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4765514
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG125670.2637071258210552RNA PCR Primer, Index 29 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76100.15968896534560595No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG59170.12416289197765444RNA PCR Primer, Index 29 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAATG20300.031.10383869
GTATGCC37950.028.81689542
TTTCGGA24850.027.9082851
GCCGTCT39450.027.37800646
TCGTATG41200.026.79491840
TGCTTGA41300.025.81119355
GTCTTCT42450.025.76831849
TATGCCG43300.025.73441543
TGCCGTC42750.025.66820345
CCGTCTT42200.025.59389347
ATGCCGT45150.024.22150844
CTCGTAT43000.024.06869139
GCTTGAA46450.023.2465456
CGTATGC48000.022.927141
CTTGAAA48950.022.2707157
CGTCTTC49900.021.92114448
TCTGCTT48900.021.80123953
CTTCTGC50800.021.60070451
TTCTGCT51200.021.1588152
GATCTTT126500.020.9997226
Produced by FastQC (version 0.11.2)