FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4209302
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA165960.39426964375566304No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG127540.30299560354662125RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG53570.1272657557001137RNA PCR Primer, Index 29 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAATG15000.032.89184669
CCGTCTT41300.028.48910147
GTATGCC41300.028.48436442
GTCTTCT41200.028.22325549
GCCGTCT41800.027.9832346
TATGCCG42400.027.74538443
TGCCGTC42700.027.63583845
TCGTATG42250.027.4356240
TTTCGGA21850.027.1603871
CGTATGC43950.026.76687641
ATGCCGT45450.026.56662444
TGCTTGA46100.025.66820155
GCTTGAA47800.025.11617756
CTTGAAA47450.024.86520857
TCTTCTG48900.024.06134650
CTGCTTG48800.024.03766454
CGTCTTC49550.023.81534448
CTTCTGC49750.023.58089451
CTCGTAT46300.023.39649839
TTCTGCT50250.023.27538152
Produced by FastQC (version 0.11.2)