FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length75
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA512.5No Hit
CCACTGACTCGCCCTCATTAGGCCTCGGTTTCCCTACGACTACCCCACACGGGTTAACCTCGCGACGCGCCGCTG12.5No Hit
AAAAAAATCATCAAAGTACTCAAAGAAACAAACAAACTTGAGTAAATCAACCATTAACTAAAACAATTATCCAAG12.5No Hit
GCGGGGGTTACGAATGAAAAGCGGATCATCAAACGCAGTGCGCAGCCGCGATAGGGCGTGGATGTTAGCGGATTG12.5No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAGATAAA12.5No Hit
AATGAAAACATCTTTGGCAAATGCTTTCGCTTAAGTTAGTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCG12.5No Hit
ACCAGCTGGTGCACCGACAGGGTGGCGTTGTAGGGCTCCACAACGGTGTCCGACACCTTGGGCGACGGCACAACC12.5No Hit
GCTTTGACCATATCGTTGCTCAGCAACCTCTCTACCGCTTTATCATCGCCATCATTATCATTACCATCCCTGTCT12.5No Hit
CAAACACACAAAATCCGAACCCATCTAAAAACCAAAAAAAAAATCAAAATCGCTGTCATCAGCACGTTAAGTGAC12.5No Hit
CTTCAGCGTGTCTGCTCGCACATCGACCATGTCCAGGAGCCCCTGGGGCGTCACATGCTGCTCGAGAACCCTTCC12.5No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAACACCACCAGAAGAAAAACAAACTAAGAC12.5No Hit
TTTAAAAACCTGCATTAGTGCTCTTAAACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTG12.5No Hit
ACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAATCCTTTAACAAGGACCAATTGCTGTCTCTTAT12.5No Hit
GAATTAACCAGACAAATCACTCCACGAACTAAGAACGGCCATGCACCACCACCCATAGACTGTCTCTTATACACA12.5No Hit
CGTCTCCCAGATGAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGACGTCTTCTTCTTGAAAAAAAA12.5RNA PCR Primer, Index 38 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACATAAAAAAACAAAAAAAC12.5No Hit
TTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGAT12.5No Hit
AAAAAGTCCAAATCTAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAACCAGATTATTTTAAAAAA12.5No Hit
TCTCCAACAAGCGTCGCATGGGTCTGACCGAGTTCGAGGCCGTCAAGGAGATGTACGATGGCACTGTCTCTTATA12.5No Hit
CCTTAGAATTCTCATCTTGACTACCTGTGTCGGTTTGCGGTACGGGCACCTAATCTCTATCTAGAGGCTTTTCTC12.5No Hit
GCTGTCCTTAGTACGAGAGGACCGGGACGGACGAACCTCTGCGTGCCAGTTGTCCTGCCAAGGGCACGGCTGGTT12.5No Hit
GCGAACAGGCCCGCGCCGACCTCGCCGTCGCCGCGCTACGCCAGCAATAACATCACTGTCGCCCCTCACCGAGCT12.5No Hit
ACCATGGTAGTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTAGTCGGTTCCTGTC12.5No Hit
ATTCTTAGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTCTGTCTCTTATACACA12.5No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAACAAAAAAAA12.5No Hit
GCTTAAGTTAGTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTA12.5No Hit
CCGGTATCTAATCGTTTTTGATCCCCTAACCTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGCA12.5No Hit
GCATTAGTGCTCTTAAACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGG12.5No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAACCACCAAAACCCCTACTAAAGGACCAA12.5No Hit
CAGAATACACGCCGCGGAATCTGTACTTCCTGCACACCAAGACCTGGCTGCTCAACATGTATGCTGTCTCTTATA12.5No Hit
CTCCTTGGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGATGCAGCAACGCCGCGTGCGGGATG12.5No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAACACAATCCACAATGAACTCGAAAAAAATCCAAAA12.5No Hit
ATAAAAATATCATATTATAATTTTTATATTAATATAAATAATAAAAATTTTTATAAAAAAATAAAAAAAAAAAAA12.5No Hit
ATAAAGAGGCTTAATATACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAACACATATAAAAA12.5No Hit
AGTCTGCCAGTACCCACCGCAGATCCGGGGTTAAGCCCCGGACTTTCACGACAGAAGCGACAAACCGCCTACGAG12.5No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAGTAGAAA12.5No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers