FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56
Sequences flagged as poor quality0
Sequence length75
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35.357142857142857No Hit
GCACACGTCTCCCTGACGAGATCGGAAGAGCACACGTCTCCCTGACGAGATCGGAAGAGCACACGTCCTGTCTCT11.7857142857142856Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCAAATAG11.7857142857142856No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA11.7857142857142856No Hit
ATTGTTAGTTACCATCATTAAGTTGGGGACTCTAAAGATACTGCCGGTGATAAACCGGAGGAAGGTGGGGATGAC11.7857142857142856No Hit
CCGACACCGACGTGCTTCCTGAGGTAGATGTGTCGGGCGATCGAGGCAGCTCGAACGTAGTACCCTGTCTCTTAT11.7857142857142856No Hit
GTCCTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATAGTATGACTGAAAAGTTTATCAG11.7857142857142856No Hit
CATCGATCTCGGCATAGTAGGCCTCCTCAACCTGGGCGTTGCGCAGATTGTCGACGGCTTCTATCGATTCCAGAG11.7857142857142856No Hit
CCGTTTCCAGCGCTTATTCCAGAGTCTGGGGCAGGTTACTCACGTGTTACTCACCCGTTCGCCACTGCTCACTCG11.7857142857142856No Hit
GAACAATTAAGTAGGGGAATGATGATGGATGATGTTGGATGATGATGCCTCCTTTACTTGTCCTTGCCGTTGGGT11.7857142857142856No Hit
GTGCTGCAGTGTGGCGCCGATTCGCTGACTGGCGATCGGTTAGGTTGCTTCAATCCTGTCTCTTATACACATCTC11.7857142857142856No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGCTGTCTCTTATACACATCTCCGAG11.7857142857142856No Hit
GTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGAATAATGAAAACATCTTTGGCA11.7857142857142856No Hit
ATAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAATAAGAACACAAGCAACAAAAATAAAAAAATTAAAAA11.7857142857142856No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACACCCCCCCGCACAAAAACAAACACCAAGAACACCA11.7857142857142856No Hit
GTTCTTCGCGTTGCGTCGAATTAAACCACATACTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCA11.7857142857142856No Hit
CTCCAAAACAAAACAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAGCACATAAAAAAAAAAAAAATAAAAAAAACA11.7857142857142856No Hit
CTCTTCAAGAGTCACTTGAGATCTAGTAGTGAAATCAGCCTGAAAGTGGCAAGTGGAAGAAGACATTTTAGGCAA11.7857142857142856No Hit
AATAAAAACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGCTGTCTCTTATACACATCTCCGAGCCCACG11.7857142857142856No Hit
ACAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCAAGGAAACGATAATAAAAAACAAACCA11.7857142857142856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAACCACAACAAGGAAAA11.7857142857142856No Hit
CTTCTTGGGGAGACGTGTGCTCTTCCGATCTTCTTTCGTATACGAGTCGACTTCCCAACTGCAAGGAGAGAACAG11.7857142857142856Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAATATCGAAAGTAGCTGCAAAAAACGCCTATTATCGTCAACGAATAAACAGAAATAGCTATAAAAATACTCTG11.7857142857142856No Hit
CGAAAGAACGGAATTGGAAAGTATACCGCTTTTAAGAACCAATAAGGCAAACAAAAGCCTAAACTGGGTAAGTAA11.7857142857142856No Hit
AATAAGACATCTGTTCTGCTTTCATTGGTCTTCAGATCAAGAGGTAATGATTAATAGAAGCAGTTTGGGGGCATT11.7857142857142856No Hit
CCTATACGTCGTCTTACGACTTCGCAGAGCCCTGTGTTTTTAGTAAACAGTCGCCACACCCTGGTCTGTCTCTTA11.7857142857142856No Hit
CATTCCAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGG11.7857142857142856No Hit
TCCTGATGCTCCGCCTCCGGAGGAGATCAGGAGGGATGAGAGCACTGTGCTGCCGATGGGTTTCGCAGATGAGCG11.7857142857142856No Hit
CTAATTGGTTTAACCCAAATAGTATTCTTAAAAATTTTAGCAAGAAAGTTCCACAATTGGCTACGTAACTAAACT11.7857142857142856No Hit
GTATATATTTGTTGTTGCTGGTTGACGACGCAGTTTTAAAATTGATGATACACAGAGAACTAAATGGATACAGAG11.7857142857142856No Hit
ATCATCAGCCCCACCTTAGACAGCTCCCTCCCTTGCGGGTTGGGCCGCCGGCTTCGGGTGTTGACAACTCTCATG11.7857142857142856No Hit
AATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTTCATTATCCCATGCACAGAATAT11.7857142857142856No Hit
CGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGA11.7857142857142856No Hit
CTTTGCAACCATACTTCCCCCGGAGCCCAAAAGCTTTGGTTTCCCGGGAAGCGACTGAGAGAGCCATAAAAGTAG11.7857142857142856No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTTCACTATCCCATGCACAG11.7857142857142856No Hit
CTTCCCGGGAAACCAAAGCTTTTGGGCTCCGGGGGAAGTATGGTCTGTCTCTTATACACATCTCCGAGCCCACGA11.7857142857142856No Hit
TGCGAATGCTGACATGAGTAGCGATAATGCGGGTGAAAAGCCCGCACGCCGTAAGCCCAAGGTTTCCTGTTCAAC11.7857142857142856No Hit
CCTCCACTATCAATAATAGTTCTAGAGTCCACGCTTGAAGCCACCATAAATGCAATACGGGTTGGTATATTAGAT11.7857142857142856No Hit
GTACAAGGCCCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCGACTTCACGTAGGCGA11.7857142857142856No Hit
ATCAGATAACGCCCTAGTTCTAACCAAAAACTATAACAGCTAGCAATTCGGAGTAGCTTCTTTTATGGCTCTCTC11.7857142857142856No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC11.7857142857142856No Hit
AGAACACAGTCTTGCCGCAACCGAAAGCTCCGCGAATGGCGGTGGTACAGCCCTGGACAAAGCGGAACAGCGAGT11.7857142857142856No Hit
GTGGTACTAAGCGTACGACAAGTAGCGCGGGACACGAGAAATCCTGTCTGACCTTGGGGGGACAATCCTCCCACG11.7857142857142856No Hit
CACCTCGATGCGCCCCACGCCAATGGTCGACAAGTCTGGATCGGTCACATACGGCACCACGCCTCACTCTTATAA11.7857142857142856No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTAAATTTAAACAAAAAACCCCCCCA11.7857142857142856No Hit
GATTGAGCAAATGCAAATACGACAGCAGTTCAAAGCGCCCTTTGTGCCGAAATCAAACTGCAAATACATGGCCTA11.7857142857142856No Hit
TGTCCGATGAGGGCAGCGAGTGCGCCGGAGCGCCCAAGTGCAACGAGGGCCAGTTTCGATGCGGGGTGAGCCGCC11.7857142857142856No Hit
GGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCACGAAAATGGATG11.7857142857142856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAA11.7857142857142856No Hit
TAGACATGCATGTCTTACTCTTTGAGACAAGCATATAACGACTGGAAGGATCAACCAGACTGTCTCATATACACA11.7857142857142856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACCACACAGTAGAGTGATTAAAGAA11.7857142857142856No Hit
TCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTTCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCC11.7857142857142856No Hit
AGAGCACACCCCCGCTGAGATCGGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCG11.7857142857142856No Hit
AGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCGGATGCAGCACCGCCGCGTGCGGGATGACGGCCTTCG11.7857142857142856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAAAA50.069.054
AACAAAA50.069.053
AAATTAA50.069.066
ATAAGAA50.069.039
CACAAGC50.069.046
AAATAAT50.069.034
AAATAAA50.069.058
AATTAAA50.069.067
TAAGAAC50.069.040
CAACAAA50.069.052
GCAACAA50.069.051
AGAACAC50.069.042
ACACAAG50.069.045
ATTAAAA50.069.068
AAGAACA50.069.041
GAACACA50.069.043
AAAATTA50.069.065
TAATAAG50.069.037
TAATAAA50.069.02
ACAAGCA50.069.047
Produced by FastQC (version 0.11.2)