FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94
Sequences flagged as poor quality0
Sequence length75
%GC20

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1515.957446808510639No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0638297872340425No Hit
AAAAACTTCACACTGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAGTAACTAAAAAA11.0638297872340425No Hit
CATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACTGCGACGTGAGAGTTGAAAAAATAGGATCGTCGAAA11.0638297872340425No Hit
ATAACCATCTGTCATCTCTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCTAAAGTTTACAGTTAAA11.0638297872340425No Hit
CAAAAAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCTGACATTTTGCATAATACCAAAACACGAGA11.0638297872340425No Hit
AAATTACCACTTGATCTCAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAATCCCATAAAAAAAATATAA11.0638297872340425No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCATAAAAAATGTAAAAATTTTTATTTTATTTT11.0638297872340425No Hit
GAAGAGAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACACAACAAGTAAAGATTAAAAATATA11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGGAGCCGAAGCGAAGGATTTTAATTTGTAT11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAACACGACACAAAAACTAAAACCTAAAAAAAGAAAAAAA11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAATATAAAAAAAAA11.0638297872340425No Hit
GTATAGAATATTGCCTGTTCGACTGAAATTATGTGAAAAGTTTTCACATTCGATCAAGTTGTTTGTTAAAAGTTT11.0638297872340425No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATT11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAA11.0638297872340425No Hit
AAAACAGCAACATCAAAAGCAAAAACAGCTAAAGAAAACATGTTCAAAATAAGCCACAGCCAAAAAGCAACAACA11.0638297872340425No Hit
CCAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGTTAAAAATCCAAGACAAAAAAAAAATAAATGACA11.0638297872340425No Hit
CCTTCAGTTGGAGAGCTGCAGAAGTTGGGAACATAAAAAAGATTCGCAGAAGTTGGGCTGAATGTTGCACAGAAT11.0638297872340425No Hit
GTGCTCTCCTTTCTTTTTTTTTTTTTTTTTTTTATTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCGCGCACGGATTAGTGAAAAGAAATA11.0638297872340425No Hit
AAAAGAAAAGAGCCTAATCCATTGTTGGGAAATTTAAGAAGTCAAATCATTTGGTAAAATGAACCAAAAAAAGTG11.0638297872340425No Hit
AAAAAAGAGAGAATAAAACACGAAACAAAAAAAAAAAAAAAAAAAACACAAAAACACATCTCCGAGCACACGAGA11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACACAAACAAGGCTA11.0638297872340425No Hit
AAAAAACACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACAAAAAAATATATATATAAAAAATAAA11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAGAGAAAAAATAATAAATTCCA11.0638297872340425No Hit
GCCCGTACCGCAAACCGACACAGGTAGTCGAGGAGAGCATCCTAAGGTGAGCGAGTCAACTCTCGTTACTGTCTC11.0638297872340425No Hit
GCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTAACGGCCCACAAAAACACTCGATTAAGAGCACTAATGAAGG11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACAGAAGGAGTAAAAAATATAGTCGATC11.0638297872340425No Hit
ACCTGCATTAGTGCTCTTAAACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAC11.0638297872340425No Hit
AATCAGCAGATCTAAAAATAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAATAGAATAAAAACAAAAAAAAATA11.0638297872340425No Hit
CAAAAAACATCATAAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAACATGAAAAAAAAAAAA11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACAACAGAGAACAAAAAAAAAACCTACACC11.0638297872340425No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTGAAACCGGATTAAAACATTTTTGCAACTGAT11.0638297872340425No Hit
ATGATGTGCTCCAGAGATGCGGCCGACGCATCCGCCGGAGAAAGCCCGTGTCCAAGCGATTCGATCCCCTGCACG11.0638297872340425No Hit
AAACCTCATAAAACTTAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAACAAAAAACAAAAAAAATCC11.0638297872340425No Hit
AAATACAAGTATTTAATCACATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACCTGTCT11.0638297872340425No Hit
AAACAGTAAATTAAAGCCTCCAAAAAGCAATCAAAATACTTAAAAGTAATAGTAACAGCACACAATAAAACACAT11.0638297872340425No Hit
AAGTAAAAACAAAGCTAGAAGAACAAAATAAAAAAATCAAAACAGACCAAAACAAAATAAAATTAAAACAAAGAA11.0638297872340425No Hit
ACACCACCCGGAACCACATCAACCAGGGATAGGAGATCCGGGAAGCGATCTCACGGACCCAGCTTAAACTCTCTA11.0638297872340425No Hit
CAAAAGAAGAAATGATAAACATTCCGAAAAAAAAAAAAAAAAAAAAAAAACCAACAGTATCTTATAAAAAACAAC11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAGACAAATGATTATTATAAAAGAATCTAATA11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAATTTAAGGACGATCA11.0638297872340425No Hit
AACCCGTACCGCAAACCGACACAGGTAGTTGAGAAGAGAATTCTAAGGTGCTCGAGAGATTCATGGCTAAGGAAT11.0638297872340425No Hit
AAACAAAACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACACACAAAA11.0638297872340425No Hit
AACTAAGTCTTAACAACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAACCCACAAACTAAAACAAATAA11.0638297872340425No Hit
GATCTGAACAGACAACACGTGCAAAAAAAAAAAAAAAAACAAATACACATATACCAAACCACGAGCCCGCTCAGC11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAGACACGCAGAGAGCAATAAAAGAAAATACAAC11.0638297872340425No Hit
GATCTAATCCGGGGTTTCAGCTTTGCAACCATACTTCCCCCGGAGCCCAAAAGCTTTGGTTTACCGGGAAGCGAC11.0638297872340425No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGA11.0638297872340425No Hit
CTTCCGATCTAACGAGGGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTGAGC11.0638297872340425No Hit
CAAAAAGATTCAACTACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAACAAGATATATATAAACAA11.0638297872340425No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAATGCCCAAACCCAGCAAGGAGATGCCAATAATA11.0638297872340425No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGACTAGACAATGTTTAAGAAAGTACTTAGAC11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACACAAAAACAAAA11.0638297872340425No Hit
GCCTTTATACTCTGCGCACGATTTCCATCCGTGCTGAGGGAACCTTTGAACGCCTCCGTTACTCTTTCGGAGGAG11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAA11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACACCAAGAAAAAAAAAAAAAAAAATAAACAAAAAGA11.0638297872340425No Hit
AATCAAAAGTTGATAGGGCAGCAATTTGAAATATCTGTCCTCCGTACAAGACAATACTATCTGAATGTTATCTAG11.0638297872340425No Hit
GCTCTAGAATTACCACAGTTATCCAAGTAACTGTTAACGATCTAAGGAACCATAACTGATATAATGAGCCTTTTG11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCAGACAAGCCCTCCAAATGGTCATAGTTA11.0638297872340425No Hit
AAATAACACACGTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACAAAAAAAAAAATAAAAA11.0638297872340425No Hit
GAGGAGAACCAGCTATCTCCGAGCTTGATTAGCCTTTCACTCCGATCCACAAGTCCTGTCTCTTATACACATCTC11.0638297872340425No Hit
AGTCTGCCCGTACCCACTGCAAGCCCAAGGTTGAGCCTTAGGTTTTCACAGCAGAAGCGACAAAAAGCATAAGAG11.0638297872340425No Hit
GTGGTGATGTGCTCCATCACAATTTTGAGCAACGGAAAATCTTTGCGCAGGGGCTCAAGCTGCTGGTCAATGAAC11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACAAAAGTAACCCCTAAAAAATTAAGATAGTAGTATAAAGGAC11.0638297872340425No Hit
GGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAACGCTTTCGC11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAA11.0638297872340425No Hit
AGTAAGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAACCACAAATAATAAAAAAAAAATGA11.0638297872340425No Hit
CTACCGAGCTGAGACCTCTAACTAAAAAAAAAAAAAAAAAAAAAAAAAAACAACCTCCACTAAAAATTATAAAAA11.0638297872340425No Hit
CAGCAATGCGGCCGCAAGACCGATCCTGCAGGCTTCGCTGGAACTGTCCGCATGCACCTTGACGGGCGCAGTCCG11.0638297872340425No Hit
CTATCGACCAGTCATAAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACTAACCAGCAGACAATGTAA11.0638297872340425No Hit
CAATAATACGGCGCCAAATAAGGACCGAACGGCGTGACAGCGACGCCTGCAACAGATGTGGAATATGACCTGTCT11.0638297872340425No Hit
AAACAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAACAAAAAAAAAAAAAAAAAAAAC11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACAACCAGCCATAATTTAAAGAAC11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAGACAATGCATGATAGAACACCAACAAAGCCACCCCAAA11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCAACACAAAAACAACAACCAAAAAAAAACAAAACAAAAACA11.0638297872340425No Hit
CTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTACTGTCTCTTATACACATCTCCGAGCCCACGAGACCG11.0638297872340425No Hit
ATTCCCCGTTACCCGTTGCAACCATGGTAGTCCTAGATACTACCTGTCTCTTATACACATCTCCGAGCCCACGAG11.0638297872340425No Hit
GTATCTGATCTCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTTTCGCTTAA11.0638297872340425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAACAAAAAAAAAAAA11.0638297872340425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGAA50.069.061
ATTAGTG50.069.059
GAAAAGA50.069.065
GGATTAG50.069.057
GCGCGCA50.069.049
CGGATTA50.069.056
AAAAGCG50.069.045
AAAAGAA50.069.066
AGAAATA50.069.069
GCACGGA50.069.053
AAGAAAT50.069.068
AAGCGCG50.069.047
AGTGAAA50.069.062
GCGCACG50.069.051
GATTAGT50.069.058
AGCGCGC50.069.048
GTGAAAA50.069.063
AAAGCGC50.069.046
CGCACGG50.069.052
AAAAAGC50.069.044