FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4307154
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG43780.10164484483257391TruSeq Adapter, Index 6 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC17100.026.4281342
CGATCTA54600.024.3259285
TATCTCG18350.024.06552936
GCCGTCT18500.023.68831646
CGATCTG108850.023.0730135
CCGATCT292250.021.3545514
TGCCGTC21550.020.81604845
TTCCGAT301750.020.8089732
TCCGATC306650.020.4757523
CTTCCGA309350.020.467651
ATGCCGT22450.020.13011244
ATCTGTG9550.019.868767
GTATCTC23050.019.15845935
GATCTAG21350.019.067586
AAAAAGG23050.019.00878366
CGATCTT52550.018.9069335
CAGTATG8300.018.70709469
GTCTTCT24800.017.39243949
GATCTGG47800.017.2496956
AAAGGGG25850.017.21872968
Produced by FastQC (version 0.11.2)