FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119
Sequences flagged as poor quality0
Sequence length75
%GC20

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2117.647058823529413No Hit
CATACGATCTGCAAAGCCATTTCACATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAGCAAA10.8403361344537815No Hit
GTCAAGATGAGAATTCTAAGGTGAGCGAGCGAACTATCGTTAAGTAACTCGGCAAAATGACACCGTAACTTCGGG10.8403361344537815No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAATTATATTA10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA10.8403361344537815No Hit
ATTCACATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGACTCACAATTACAGTTAATTCCAAGATCATGAGAA10.8403361344537815No Hit
AAAATAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA10.8403361344537815No Hit
ACAATGATCACCTAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACAAAACCCAACAGAATAA10.8403361344537815No Hit
GTGTTGCTGTCCAGCCATCGAATAGTTTCGTTGCACTTGTCCAGGACGGAGTTCTTGTCGGCCTCGTCCAGTTTG10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAACACAT10.8403361344537815No Hit
CATTAGCATGGAATAATAGAATAGGACGTGCGGTTCTATTTTGTTGGTTTCTATGATCGCCGTAATGATTAATAG10.8403361344537815No Hit
CCCTAATAAGATCGGAAGAGCACACGTCTCCCTAGGAAGTTGGGTAGACCCAACTGCTCGCGAAAGAGAACGATA10.8403361344537815Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AACATAGTGGTTGCGCTCGTTGCGGGACTTAACCCACCAAATAACTGCACGAGCTTACGAAGGCAATGCAGCACC10.8403361344537815No Hit
AAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAACTAGAAAAAAAAAAAAAATACCA10.8403361344537815No Hit
GACCAAGATACAACAAACAAAAAAAAAAAATAAAAAAATTTAAAAAAACAGTCGTCGGTACAAGACCATACGAAC10.8403361344537815No Hit
CTTCACGACTACTACAATTTCACCGGATCCTTTGTTGAGACAGTGCCCAAATCGTTACACGTTTCGTGCGGGTCG10.8403361344537815No Hit
TTGTAACAAATAACTGTACGAATTAAATAAAAAATAAAAAGGATGGCGCTTAAGCGTGTTACCCATACCTCGCCG10.8403361344537815No Hit
AATTGGTACCGTAACTTCGGGAAAAGGTACGCCCCGGTAGCTTGACCACTTTACTGTGGAAGGGTGAAAGGGTTG10.8403361344537815No Hit
CTTCAGACCCTATACGTCGTCTTACGACTTCGCAGAGCCCTGTGTTTTTAGTAAACAGTCGCCACCCCGGGGTGT10.8403361344537815No Hit
AATAATATATCAAAATATTTTTTATATTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAATAATAAAAAAAAA10.8403361344537815No Hit
GAGCACACATCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCGAAGATAATATAAAAAAAACCGAGAAGCGT10.8403361344537815No Hit
ACATACACAGATCATATAACAAATAAAAAATAACAAAAAAAAATAAGGCTAAAGTGTATCTAACAAATTTTCTCA10.8403361344537815No Hit
GAGGCGGCCGAGGCTGAAACACCTGCGCCTCGATCCGCTCCTTCAGCATGTCCGCGTTCACCCTGTCTCTTATAC10.8403361344537815No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTGCGGGACTAAACCAAAAATATCAAGAAAAGAGCT10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAGCGGCAACGCTGGATCAGGATAACGCCAATAGTCC10.8403361344537815No Hit
CACCTCTCCGAGCCAACGAGACGGAGCTACATCTCGTATCCCGTCATCTGCTTGAAAAAAAAAAGGGGCGGGTGG10.8403361344537815No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAGTTATCCACAAAAAAAAACACAACTAAAAACCAACAACACCGAACA10.8403361344537815No Hit
AAAATCAATACAACACATAAATAAATAACACATCAGTTCTGCTATAAATAAAAAAAAAAAAAAAAAAAAAAAAAA10.8403361344537815No Hit
GCTGACAACATGGTGAAATCCACATTCCTTAACGCGGTGAGTGCACGAGAGAAGAAATTCCCCACCTTAGATCGG10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAGGGACCAAAGGAATATAACA10.8403361344537815No Hit
GCTTTAAACGACCCGTCTTGAAACACGGACCAAGGAGTCTAACATATCTGCGAGTATTTGGGTGTCAAACCCAAG10.8403361344537815No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGGACGACCACAAAGCAAAAAACAAAGCAAAAAGCGAACGC10.8403361344537815No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCACTTGATCTGCTGTCTCTTATACACATCTCCG10.8403361344537815No Hit
ACATAAGGCTGCTCAACCTAAAAAAAAAAAAAAAAAAAAAAAAAACAAAATGACATTGCCGTACAAGTGATAGCA10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGACACCACAAAAAAAAATCAAGGTACGAGAATAAA10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAACAAAAAAAACAAAAAACAAAA10.8403361344537815No Hit
AATAAAAAATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACACACACACAAATACACATCTAA10.8403361344537815No Hit
AAACAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCGAGAGAACACACGAAAAATAAA10.8403361344537815No Hit
ATAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAGAAAGGAACCACCAACTAGAAGGATCGATAGGACTATAG10.8403361344537815No Hit
CTTCCGATCTGAGCCGGGTATTTAGCTTTAGATGACAATTACCACATTTTAGAGCTGCATTCCCAACCAACTCGA10.8403361344537815No Hit
AAATATTCTTTTCACAAAATCCGAATCAGATACATGTGAATGTTTGATTTTTTTGAGGATTTGTATGTAAGTGAA10.8403361344537815No Hit
AAAAAACGTTACAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAATAAAATAAAAAAAAAATA10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAATAGAGATGATAATT10.8403361344537815No Hit
CTACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGACGAACCACCCCCCATGTTAAGACAAAATTTCT10.8403361344537815No Hit
AAAAAACAACAAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAATAAA10.8403361344537815No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8403361344537815No Hit
CTTTTTTTTTTTTTTTTTTTTTTTGGGAATGGACCACCCCCCAGGCCATGAAGTTATAAAAAATAAAGAAGCCAA10.8403361344537815No Hit
GCCCTGCCCTACGCCAACGGTTCGATCCACCTTGGCCATATGCTCTAGTACATCCAGACCCACATGTGGCTGCGT10.8403361344537815No Hit
CAAAAATAGAAACATAGATAATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACAAAAAGACAATAAAAAAAA10.8403361344537815No Hit
TTTCTCGACAGCATAGGATCATCCACTTCACCACAATCGGCTCGGCATCAGGTCTCAGCCTTGCGCGGCGGATTT10.8403361344537815No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACATCCGCACTCTCACTCTTCTCCATCATGGGAATCGC10.8403361344537815No Hit
CCCTAATAAGATCGGAAGAGCACACGTCTCCCTAAGAAGTAAGGAAGAGCCCTCGGCGCGCGAACCAGACCGGTC10.8403361344537815Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAAAACAACTTTGCAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAGAAAAAAAAAAATATAATAAAAA10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT10.8403361344537815No Hit
GCTCCGTAATCGGAATGAGTACACTTTAAATCCTTTAACGAGGATCTATTGGAGGGCAAGTCTGGTGCCAGCAGC10.8403361344537815No Hit
ACACACTACAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGATATTTAACAAAAACACATCACCGAGCCA10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACAACCAGGAACAAGAGAA10.8403361344537815No Hit
GATAAGACTACAACGGTATCTAATCGTTTTTGATCCCCTAACCTTCGTTCTTGATTAATGAAAACCTGTCTCTTA10.8403361344537815No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGAACAACCCCTAAGAAAAAAAAAAAAAACTAACAAAAAAA10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAACAAAAAAAAAAAA10.8403361344537815No Hit
TTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCT10.8403361344537815No Hit
GCAGAAACTCGCATCAGATTTATGAGCTAAAGCTAAAGATTAGACGCCTAGCCGTAAACATCCTTAACCTATTCT10.8403361344537815No Hit
ATCTGGCAGATCACGGAATACCTCGGTATCGCTTGCTTCACTGTGCGACACAAGGACCAGACGGGTCATTGGGTG10.8403361344537815No Hit
TCAAAATGAAATAAGAACTAACTAAAAAATAAAAAAAAAAAAAAAAAAAAAACAAAAAAATAAAAGAAGAAGTTT10.8403361344537815No Hit
CTAACTAAAAAAAAAAAAAAAAAAAAAAAAAACCTACAACGAACCACCAGCATATATCAAGACATCAAAACGGAT10.8403361344537815No Hit
CAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAGAAATCATAAATCAAAAAAGAAAGAAATAGGTGCGAAGGCGAA10.8403361344537815No Hit
ACACACGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACCCTGCAATAAGAACTATAAAAAAT10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACACAAAACCCCCCACACGAAAAACAAAAAGATACAGCAAGA10.8403361344537815No Hit
ACAAAAATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACACAAGATCACCAACGAAAGTTT10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAGAAAAAACTATAAAA10.8403361344537815No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGAAACCAGAAAAATAAAAAAAAAACACACGGCCAG10.8403361344537815No Hit
ACAACACACATATATACCAAAAAAAAAAAAAAAAAAAACAAAAAAAACTAAAAATTTTAACAAAAAACGCTTACA10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAA10.8403361344537815No Hit
ATCATAAGATCCTATACCAAAAAAAAAAAAAAAAAAAAAAAAACAGATAAACAGCTTCCAGGAAAAAAATATAAA10.8403361344537815No Hit
AACAACGGGAAATCATAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCAAAAAAAAATTAAAAAAAAA10.8403361344537815No Hit
AACCTATTCATATCAAACAATTGGTTCTCAACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAATG10.8403361344537815No Hit
GGTTACGGCTTCACTGACTCTAGTACAACAATTTCCATGGGTATTTTGGCAGCAAGTTAAGGATAGCACATGGTA10.8403361344537815No Hit
GTAAACGGCGGCCGTAACTATAACGGTCCTAAGGTCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGG10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCAAAGTATAAAGTAATTGATAT10.8403361344537815No Hit
TCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTAAGGCAAAAAGAACCAAACGGATTTAGGAATAGG10.8403361344537815No Hit
AAACATATAAATGTAAAACTTGGGACATTCTACAAAACCTGTCTCTTATACACATCTACGAGCCCACGAGACCGA10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAATAAAAAAAAAAAAT10.8403361344537815No Hit
AATCACAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACGGATAAATGCGCTGTTATAAGCATATCTCT10.8403361344537815No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACAAGCACCCCAGGGATGATAATAAAAATTTA10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTACAAAAATAAAAAAAAAAAAGAAACA10.8403361344537815No Hit
GTAATTGGAATGACTACACTATAAATCCTTTAACAAGGACCAATTGGAGTGCAAGTCTGGTCCCAGCAGCAGCGG10.8403361344537815No Hit
ACTATCTAGAGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGA10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGCCAGGGGCCAAGATCTGTATAAAATACACATCGCCGC10.8403361344537815No Hit
CCGAGCCCCCGAGACTGCAGCTACGCGCGTATGCCGGCGGCGGCTTGCAAAAAAAAAGGGGGGGGGGGGGGGGGG10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGCAAGGAAGTTTCCACCGCCGACCGGAAACCAAGAAAATAAAACA10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCCACCAAACGCCTTAAAAAAAAAAATAACAATAACA10.8403361344537815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAA10.8403361344537815No Hit
AAACAGAAGAGCAAACGAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAACCAAAGAAAGA10.8403361344537815No Hit
AAAAACAGCAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCAACAACCCAAGATATAAAAAACA10.8403361344537815No Hit
TCTATGTGCCATCACGATCTTGTACGAAATGGTTTTTATCGACCCGTCTTGTAACACGGACCAAGGAGTCTAACA10.8403361344537815No Hit
ATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGGTCTAGAAAAAACAAAACCACTTTGACAAATGCATAC10.8403361344537815No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers