FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length75
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA211.76470588235294No Hit
CTCTAATTTGTTCAAAGTAATAGTACCGGCCCACAATAACACTCGTTTAAGAGCACTAATGCAGGTATTTTAAAT211.76470588235294No Hit
AAAAAAAAAAAATCAAACAAAAAAAACAAACCAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15.88235294117647No Hit
CTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATG15.88235294117647No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGT15.88235294117647No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAATAAAAATAAGAAAAAAAAA15.88235294117647No Hit
TGCCAGTAGTTATTTGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGAACACACGAATAAAAAGTGAAACCAA15.88235294117647No Hit
GCTTGAAGCTGATGGCAGAGGTCTTGAAGGTGGAGGTGGTAGTCAGGGTGTAGGTATCGCCCTCCAAGGTCACCT15.88235294117647No Hit
AAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGAGGTTGCTGACACAAATACCAATATAGGAGG15.88235294117647No Hit
CTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATC15.88235294117647No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACAAAAAAAAAC15.88235294117647No Hit
CCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTTCAT15.88235294117647No Hit
GGTTGAACTCTAGATAACATGCAGATCGTATGCTCTTGTACCGACGACAGATATTTCAAATGTCTGACCTATCAA15.88235294117647No Hit
TTGCGTGCACGAAGTGCTTTATTTAACCGATAAAATAAATGCTTCACAATTTATCGTTTTCTGTGCATGTGCTCA15.88235294117647No Hit
AGACAGGTGACAGCACAACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCAAGCGCATAAAACGATAGTA15.88235294117647No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers