FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length75
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA311.11111111111111No Hit
GGAACTCATAGTGCAGCATAAAGTTCTTGGCCTTCAGGCCACCGCTATCTAAAGCAGCCAACGAGTCAAGTTTCA13.7037037037037033No Hit
AGTCCTTCGAGAGGGACTTTTGCGACCTGCCGCCCTACCAATGCGAGATGAGTGATTCTGCGCCCAATACCCCAG13.7037037037037033No Hit
AATAAAAGCACATGTCCCATAAGGTTCATGTTTTAATTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAG13.7037037037037033No Hit
CAACAGATCTCTACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCGACCCCAAAAGCATTGGTTTAAAGGCA13.7037037037037033No Hit
ATAGGAATGAGTACACTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGAAGCCGCGGTAA13.7037037037037033No Hit
CACAGAGCCTCCACGTCTGAAATGTCCCATGTGTCCAAGTATATACTGTCTCTTATACACATCTCCGAGCCCACG13.7037037037037033No Hit
AGGTGTTGGTATAAGACTACAGTCAATGTTAATCGGTACTGCACGCCAGTCGCACATTTTCTACTGCGACAGGCA13.7037037037037033No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGCTGTCTCTTATACACATCTCCGAGCCCA13.7037037037037033No Hit
CAAAATAAATTGTACTCATTGCAATTAACAAAAAAAAAAAAAAAAAACAAAAAAAAAAAATTATAAAACACTACG13.7037037037037033No Hit
GTCCTAGATACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG13.7037037037037033No Hit
CCCCAAGTGCACCGCCTCCATGCGGCGCCGTGGCAACCGAAAGAACTGAGGCTGACTGCGCCTGCTGGCGCCCCT13.7037037037037033No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGG13.7037037037037033Illumina PCR Primer Index 10 (96% over 28bp)
GCCCACAATAACACTCGTTTAAGAGCACTAATGCAGGTTTTTAAATAGGAGGAACGTATGAAAAGATACAAGTAT13.7037037037037033No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGATCTC13.7037037037037033No Hit
TCCAGGTACTCCACTGCCACCCGTCCCTCGATGGAGGACATCACGTAGCCTTCTTTGTCTGTCTCTTATACACAT13.7037037037037033No Hit
GAATGTGAGATAAAAGGCGTACTTATATTTGGTTGGCGCATCCTTGACGGGCGTAAAAATAAATAGATAGACCTG13.7037037037037033No Hit
TAATAAAAGCACATGTCCCATAAGGTTCATGTTTTAATTGCATGTATTAGCTCTAGAACTGTCTCTTATACACAT13.7037037037037033No Hit
GGAGAGGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAACAATGAAAATATAAACAAAAA13.7037037037037033No Hit
GTTGAAAATGGAGCTGGAACAGGATGAACTGTTTATCCACCTTTTTATCCGCTGGAATTGCTCATGGTGGAGCTT13.7037037037037033No Hit
AGTTCTAACCATAAACGATGCCAGCTAGCAATTGGGTGTAGCTACTTTTATGGCTGTCTCTTATACACATCTCCG13.7037037037037033No Hit
AAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTT13.7037037037037033No Hit
GCTCTCTGCAAACACGAAAGTGGACGTATAGGGAGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGGGGTGC13.7037037037037033No Hit
ACAAAGCTCCAAACTTCGTGTGGATCAGCTGAAATATGACCTGAGACACCTGCAGACATCACTGCAGACGGCGCG13.7037037037037033No Hit
CTCATATACGAGGCCCTGTAATTGAAATTAGTACACGTAAAATAATTAAACAAGGACCCAATGGAGGGCAACACT13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers