FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length75
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT17.6923076923076925No Hit
GAGGCAGAATCGCCGACATGGGGCTTCTCAATATCCTTGGGTGCAGCAGCCATTATTTAGCAATTTCCCTGGTCG17.6923076923076925No Hit
CATCTGATGGTGGTGATGGTGGCTGTGGAAGGGGTGTTGTCCGAACCCGGAGCCCAGCTCCGCGGCTGTCTCTTA17.6923076923076925No Hit
CCATAACTGATATAATGAGCCTTTTGCGGTTTCACTTTTAATTCGTGTGTACTTAGACATGCATCTGTCTCTTAT17.6923076923076925No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG17.6923076923076925TruSeq Adapter, Index 11 (96% over 30bp)
AGTCTAAACATGGAGAAAAGCTGCAGTATTGGCAACGGACGGGAGCAATACGGCTGGGGACATGGCGAACAATGC17.6923076923076925No Hit
TCCAGGGCGAGCGTCTGTATCCGATGATTGAGCATATGCACGCCAATTTGGCTGGTAAGATCACCGGCATGTTGT17.6923076923076925No Hit
CCCATCTCCCGCTCGGCGAACTTGCGGATCTCCTTGATGGCGCGGGGCGCGCGCTTCTTGAAGCCAATGTTGTGG17.6923076923076925No Hit
GTACACTTTAAATCCTTTAACAACTGTCTCTTATACACATCTCCAGCCCACGAGACACTGAGCGATCTCGTATGC17.6923076923076925No Hit
TTTAAGAGCACTAATGCAGGTTTTTAAATAGGAGGAACATATGAAAAGATACAAGTATTTAATCACATATAAGAG17.6923076923076925No Hit
GCGTAGGGGAGACGTGTGCTCTTCCGATCTGCGTAGGGGAGACGCTGTCTCTTATACACATCTCCGAGCCCACGA17.6923076923076925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCTGCTGGCACCAGACTTGCCCTCCAATTGGTCCTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGG17.6923076923076925No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAATGACCAAAAA17.6923076923076925No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)