FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length75
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA512.5No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAACAAAAAAAA12.5No Hit
AAAACAAAAAATTTCACTAAAAAAAAAAAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAACATAA12.5No Hit
CTACCCATCTTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCAACAACACCGCTAGAAGAAAACCAAAA12.5No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCCGCAGCCGCGGTAATTCCAGCTCCAATAG12.5No Hit
GGGTAACCAAAATAGTATTAATAAAAATATAAGCAACATAGTTCCTCAATTGCCTACGTAAATACACTATACGGG12.5No Hit
GCGTATTACCGGTGGAGTTCTTATATGTGATTAAATACTTGTATCTTTTCATATGTTCCTGTCTCTTATACACAT12.5No Hit
AAAAACATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATATATAATATAA12.5No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAATCCTTTAACAAGGACCAATTGGAGG12.5No Hit
GTAAATAATAGATTTAATTAAAAAAAATCGGAAATTGACCTCAATATTATACACAACTACGCGACACCAATCTAC12.5No Hit
CATACAATTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCAGCAACTGCTCATCATAAAGAAAACAAGCAC12.5No Hit
TACTAACTTAAGCGAAAGAATTTGAAAAAGAGGTGTTAATTACTCAATCACTAAAGTTAGAGTGGCGCAGGCGCT12.5No Hit
AATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGACGGAAAACACATTGGTAAGAATGCAAACAAAGAGAC12.5No Hit
ATAAAATATATTATATTAAATTTTTTTATTTTAAAAATTTATTATTTTAAAGTGTGAGTAATAATTGGATGAAAA12.5No Hit
CTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGA12.5No Hit
GAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATAATGGTTGAACTCTAG12.5No Hit
ATCCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACCAAAGTTATAAAACTAACTGTAAACGA12.5No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAATAGAACCTGACTCATAGACACATATACAACCCCACGAGACACAGAGC12.5No Hit
ATTCGAAAAAAATAAAAAAAAAAAAAAGAACAACCAGCCGACATCTCAAACCACGAAACCACAATCCCACCACGA12.5No Hit
AAAAACCTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGACAAAAACACCGCAGATAATAAAAATAAAAA12.5No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGGAGGCGCCCCCCGAGGTGGAGTAAAAAAA12.5No Hit
AAGAAAAACTAATCACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAATAAAATAAAAAAAA12.5No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACCACACACGAATAAAAAACACCAAGCCAACCAAACACA12.5No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACATGCGCAACAGCGTGGCATAGCCCTCAGCCGCCGACATC12.5No Hit
AAATTATTAAAATTTATAAATTAATTATAAATAAATAAATAAAAAAAAAAAAAAAAAAAAAATAAAAAATAAAAA12.5No Hit
ATTATCTAAAGAAAAAAAAAAAAAAAAAAAAAGCATGACCAGTCTCATATAAACAACTCCCAGCCCACGACACAC12.5No Hit
GATCTAGACTGGGATTCTGGTTGATCCTGCCAGTAGTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA12.5No Hit
CTATGAAGCTCTACGCCCTGTTCTCCCTTCTGGTGGGATATTTGTCCAATGGTCACATTTCCGCCGACTCATATA12.5No Hit
TTACCACAGTTATCCAAGTAACTGTTAACGATCTAAGCAACCATAACTGATATAATTAGCCTTTTGCGTTTTCAC12.5No Hit
GACCTAGAAACTACCAAAAAAAAAAAAAAAAAAAAAAAAAACAAAGAACACTCGTCGGAAAAACACCATAACAAC12.5No Hit
GCAGGGGATAATTAAATAAGACCTATGTTCTGCTTTCATTGGTTTTCATATCAAGAGGTAATGATTAAGATCAGC12.5No Hit
CCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAGCCCAAAAGCTTTGGTTTCCCGGGAAGCGACTGAGAG12.5No Hit
TCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTATATCCTGTCTCTTATACACATCTCCGAGCC12.5No Hit
ACCACCTAATGATAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAACAAAAAAATAAA12.5No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCTTATAAACATCTAAAAGACAAAG12.5No Hit
CTTCACCGCCGTGCTGAAGAAGGGCAAGTATGCCCAGCGCCCCGCCAAGAACACCGTGCGTGTTGACTTCAAGGC12.5No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers