FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97
Sequences flagged as poor quality0
Sequence length75
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88.24742268041237No Hit
AATAAGAAGATCAAACAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAACAATGCCAGCTAGCAATTAAATATAA11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAGCCACATTAAAAAAGAAAAGATAAAATAAAACGATAACA11.0309278350515463No Hit
AAAAATAAGATATCGTAACATAAAACGTAAAAACTTACGTGCAAAACAGAAAAAAAAAAAAAAAAAAAAAAAAAA11.0309278350515463No Hit
GTAGTACAACTTACAATTGTGGTTAGTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATG11.0309278350515463No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTGGGCTTTGCAGATCCTGGCTGATAAACACATAACCGACCCAA11.0309278350515463No Hit
GAGGGAGCCTGAGAAACGGCTACCACATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA11.0309278350515463No Hit
GTCTTCAACTTATCCTCCTTTGCCAGCTCAGCTGGCTTCTGGAAGTACCATTTCAAGGATGGCTTTGTGACCAGC11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAA11.0309278350515463No Hit
AACTAGTAACGACTCCACATCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATATAAAAGAATACCATTGT11.0309278350515463No Hit
CACCAAGAAGGTGCACCAGTATTGCAAAGGCATTGCCAAATATAATCAATGCGCTCTATGTCCCCTTGAATATAT11.0309278350515463No Hit
CATATTACATCGCATTATAACAACAAGAAGAACCGAAACAATATATAATATATACAAATCCAACCAGCAGCAACA11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAGCACACGACATATAAACCCAAAACCAACACACAGATCAACAGA11.0309278350515463No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAGACACAAAAAGATAAAAAAAATAAAGATCA11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAACAAATATGAAAAAAC11.0309278350515463No Hit
GCTAAAAAATTACCACAGTTATCCAAGTAAATGAAAACGATCAAAGGAAACAAAACTGATATAATGATCATTTAC11.0309278350515463No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATTTGCTTTTATTAGGCTAAAACCAAGAGAA11.0309278350515463No Hit
AACCATAACTGATATAATGAGCCTTTTGCGTCTGACTCTTATAAAAATATCCGAGCCCACCAGACACTGGCGCTC11.0309278350515463No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGACAACAATAGAAATCACAACAG11.0309278350515463No Hit
AAAATAAGTATTTTTGCTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGCAATCATACGATTT11.0309278350515463No Hit
CTTCCGATCTAGGCTGGGAGACGTGTGCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGGCACTCGCGAATA11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGAACAACACCGCAAAAAAAGAAACAAAAAAA11.0309278350515463No Hit
CTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACCTGTCTCTTAT11.0309278350515463No Hit
GTGAAGAACATTCAGAAAATTACGCAATCGATGAAGATGGTGTCCGCTGCCAAGAACGCTCGTGCCGAGCGAGAA11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGA11.0309278350515463No Hit
ATATTACGTGGCATTCGGAGTTCATCAGGATTCGGTAGGCTATGACACCCCCTAGTCTGTCTCTTATACACATCT11.0309278350515463No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAA11.0309278350515463No Hit
GACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAA11.0309278350515463No Hit
GGCTTGGAATTTAAGTTTCAGCTTTGCAACCATACTTCCCACGCAGCCAATAAGATTTGGTTTACCAGGAAGCTC11.0309278350515463No Hit
CAATAATGAATGAATAAAAAGCGTCTGATTTCAGTGGATTTCAGATCAAAATGTAACGTGTTATAGAATAAAATT11.0309278350515463No Hit
AGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAATAGCGTATATTAAAGTTGTTGCGGTTAAAACGTTCGT11.0309278350515463No Hit
GCTTAGTACGAGCCATGTTTTCTTAGCTTTTTTTTTTAAATTCTACAATCACTGTTTGGTTGGTACCCAAAATAA11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCACAAAATAAAATAAACAAAAAACAACAACCCAGA11.0309278350515463No Hit
GTAGTATCTACGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA11.0309278350515463No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCTGGGCTTCAATTCAGAGCTTGCACTCCTCCTCTTAACCTT11.0309278350515463No Hit
CAGCATAGAGTACCCCGGATTTGCCTAAGATACATGCCTACAACCTTCCACCTGCACAACCAACCCCAGGCTGAC11.0309278350515463No Hit
ATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAAGGTTCATGTTTTAATTGCA11.0309278350515463No Hit
TGCCAGAGCTGGGCGACGTAGCCTCCTCCGACTTGGCCCGTTACTGTTTGTCCTTCTTGCCAAAGGAAATGATTC11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAGAAAAAGAACAAAA11.0309278350515463No Hit
TCCCAAGGAGGCGGTGGAGCAGGACAATGGCAACGATAAGTAGAGGACCTGTCTCTTATACACATCTCCGAGCCC11.0309278350515463No Hit
GCCATGCACCACCACCCATAGATTCGAGAAAGAGCTATCAATCTGTCTTACACACTTATGTTCCTGTCTCTTATA11.0309278350515463No Hit
CCATAGTGTAATCATCCAACATGGGCAAGGAAAAGATTCACATTAACATTGTCGTGATCGGACACGTCGATTCCG11.0309278350515463No Hit
CCCACGGCAGATCGGAAGAGCACACGTCTCCCGCGCGAGATCGCAAGTGCACAAGTCTCCCGGCCGCTGTCGCTA11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATCTCAACCGAAATAAAATTTATAATATTATATATTTTATAATTTTATATAAAAAAAAAAAAAAAAAAAAAAAA11.0309278350515463No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTAAAAAAAAAAAAAATGTTTTTTAAAAAAAA11.0309278350515463No Hit
GTGCAACTAAAGGCCTAAATAGCCCATACCTACCTTTTTTGTAAACAAGTGAACAAGTTCGAGGACTGTCTCTTA11.0309278350515463No Hit
ACAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAACCCAATATCAAAAATAAAAAAAAGAGAACAACAA11.0309278350515463No Hit
CTACTAAATATCAATAAATTAAAAAAACAATAAAAAATTATATATAAAGTAACCCCAAATATAATATGAAACATA11.0309278350515463No Hit
GTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAA11.0309278350515463No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCAGGCTTCTGCATGAAAAAAAAAAGAGGGGGGGG11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGACTCCGACCCCGGAGAAAAGCAGAAAGACACCAACCGGCGG11.0309278350515463No Hit
AAAAATAGCACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCAAACATTAAAAAAAAAAAAAAAAA11.0309278350515463No Hit
ATAAACAAAAGAACAATTAAATATTAAATAAATATATAAATTTTAATAAAAAACACTTATAAAAAACTCATAGCC11.0309278350515463No Hit
CAATTACAAAAAAAAAAAAAAAAAAAAAAAAAAATACAAACAACAAAAAAAAAAAAAAAAAAACAAAAAAAAAAA11.0309278350515463No Hit
GAAAAAGCCCACGGCGTTGTGGCCGTACCACCACTGCACCATGGCGTCTTGCACCCCGGCGTAGGCGGAATAGGA11.0309278350515463No Hit
TGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATG11.0309278350515463No Hit
GCTGATGACTTGGGGTAGAGCACTGAATAGGCTAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT11.0309278350515463No Hit
ATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTTGTTTTAGCCTAATAAAAGCACATGTCCCATAAGGT11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAATAAAAAAAATT11.0309278350515463No Hit
ATATTCAGTGGCGAGCTTAACCGAATAGGGGAGGCGTAGCGAAAGCGAGTCTTAATCTGTCTCTTATACACATCT11.0309278350515463No Hit
CTCTTTCAACAGCGTCCGGACAAGGTGACGCTGTTCCTGCGGCCGGAGCTCTACTTACCCGGACCCCAGATGGCG11.0309278350515463No Hit
GCCGTCGCCGTACCGTTATCCTTCGCGAACTTCAAAGGACACTCCTTTGCACTGCTCTCGCCCATCATCCTTGAT11.0309278350515463No Hit
GACATATCCTGTTGTGTCGATATTTTCTCCTCGTCCCTTGAAAATTTATGGTGGGGACAGGCAAAATTCTCAACA11.0309278350515463No Hit
ATCTCTGGCTGTGGATATCGATCTTGTGGCGCTTGAAAAATTCAGCGAACGTCGCGTCAGTGTAGTCGCTGTCTC11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAACGCAGAATCAAACAGAATCCAAAGGCCTG11.0309278350515463No Hit
GACACACATCAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGACACCAACACCGATGTATAAAAAAAAGC11.0309278350515463No Hit
ATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAGGAAATACACGTTGATACTTTCATTG11.0309278350515463No Hit
AAAAAAAAGATCGGAAGAGCACACGTCACCCGGAGGAGATCGGAAGCTGTATCTTATACACATCACCGAGCCCCC11.0309278350515463No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAGAAAAAA11.0309278350515463No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC11.0309278350515463No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAAC11.0309278350515463No Hit
AATAAATACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGAAAAAAAAAATTAAAAAAAAAACTCAACCC11.0309278350515463No Hit
CATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAACAACCCCCCCACGACAAAAAAAAAAAGAACAAG11.0309278350515463No Hit
AATGAACCATAACTGATATAAATAGCCTTTTGCGTTTTCACTTTTAATTACTGTGTACTTAGAAATGCATCACTT11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAGAAAAAAATA11.0309278350515463No Hit
CTAGATAACATGCAGATCGTAAGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATG11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAGCAAAAGGCACAAAAAAAAAATTAAGCTACCAGAG11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAACAAAATAAATGAAAAATAAACAAAA11.0309278350515463No Hit
GCATTAGTGCTCTTAAACGAGTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGG11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACAAAAAAAAAAAAAAAAAAAACAAAAA11.0309278350515463No Hit
AAAGAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAATAAAAAAAAAAAAAAAAAAAAA11.0309278350515463No Hit
ACCAATTGCTGCTCATCCTAAAAAAAAAAAAAAAAAAAAAAAACAAAAGAACCCCCAACACAAGAAAAAAAAAAA11.0309278350515463No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCCTGTCTCTTATACACATCTC11.0309278350515463No Hit
TCGTAATACTAATGCCACCAAACTGCTTCTATTAATCAATAAATCTTGATCTTAAAAACAATGAAAGCAGAACAC11.0309278350515463No Hit
TAATAAAGCTTAACGAAGGTTGTTGACACAAGTATCAATCAAAAACGCTTCTAATAAAGAAATACGGAATAACAA11.0309278350515463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAATTATAAAAAAAAAAAAAATAAAAAAAAAAAAAA11.0309278350515463No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAAGCCACCCCCGGTGGAAACATTTTTTTTATAAAATTTT11.0309278350515463No Hit
AAGAACAACAGATCATTCACAAGCATATACATATCAAAGAAAAAAAAAAAAAAACAAAAAAAAAAAAAACAACCA11.0309278350515463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT50.069.059
AGCCTAA50.069.066
TGCATGT50.069.013
CCAATAT50.069.034
ATCTTGC50.069.045
TCTAGAG50.069.022
AACGATC50.069.041
GCGATCG50.069.050
TTCAACC50.069.029
CCTAATA50.069.068
ACCATAC50.069.02
GCCTAAT50.069.067
CAACCAA50.069.031
ATCTAGA50.069.021
TTATCTA50.069.019
TAGCCTA50.069.065
TAACGAT50.069.040
TGCGATC50.069.049
TATAACG50.069.038
CGCTTGG50.069.055