FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length75
%GC21

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38.823529411764707No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAGCACAAGAAAAACAAAAAAATAACAA12.941176470588235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAACAAGCAGATTTAAACAATT12.941176470588235No Hit
GAATAGCACAATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACGAAACACAAACTTAAGATAAAGAAT12.941176470588235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACACCAACCACGCCACCAACTGTCTCTAATAAA12.941176470588235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCGAAGCGGCCAGACGACACCTGAAAG12.941176470588235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGATGGAAGAAAAAAAAAA12.941176470588235No Hit
GGCTTAAGGTCAGAGAAGATTACTCATTAGAATGTGACCCAGCCGTCATAGGAACAGCTGTTAAGGGAAGGGAGG12.941176470588235No Hit
AAGTAAAATATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGAAAGAAGAAATTATTATAAAAAAGTA12.941176470588235No Hit
GCAGTGGGAGACGTGTGCTCTTCCGATCTGCAGTGGGAGACTTGGGTTAATCCGAAGTCCAGAGCGAGAGTCGCT12.941176470588235Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGAATTTATTCAAATCTATCTCGCGCTCATCCACGCTATCATCCGATCCGGATTCATCGTTGCGGGCCTTGTT12.941176470588235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAACCCAAAAAAAAAAAGGAAATAA12.941176470588235No Hit
ACACTCGTTTAAGAGCACTAATGCAGGTTTTTAAATAGGAGGAACATATGCTGTCTCTTATACACATCTCCGAGC12.941176470588235No Hit
CAAAAAAAGTGTACTCATACCAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAGTAAATAAAAAA12.941176470588235No Hit
ATCAACAAAATAAAAAAAAAAAAAAAAAAAAAACAAAAAATCACATACCGGCAAACCAAAGCTAATGAGCTCAAG12.941176470588235No Hit
ACTCAAGACACAAAACGAACTACATAACGAACGTCATTTACACGCAAACCACACACAAACTGACTCTTATACACA12.941176470588235No Hit
AAACACATCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAATGAGTCTTTAAAAAATACCACGGGAACA12.941176470588235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAA12.941176470588235No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCAGTTT12.941176470588235No Hit
AAGCAGAACAGAGGTCTTATTTCATTATCCCATGAACAGAATATTCAGGCATTTGAAGCATCCTTTAAGCAGAAT12.941176470588235No Hit
AAAAACAAATAAAAAAAAACTAAACAAAATACAAATAAACAGAATAAAAAATTATTGCAAAAAAAAAAAAAAAAA12.941176470588235No Hit
AAAAAACCAAAAACCACCAGTGCAAAACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAA12.941176470588235No Hit
AAAAAACATAAATATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACCGCAAAAAAAAAA12.941176470588235No Hit
AATAATATCTGAAAAAATACAAGTATCATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAATA12.941176470588235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAATCATTACAAATTAATAT12.941176470588235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTACCCAACTAAGGAACATGACAATAAAACACAACACCGAGC12.941176470588235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAATAAATATTATGTGCAAGAGATAATG12.941176470588235No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGCTCCCTTATTCCCAGGTCCGCGGAGACTCGA12.941176470588235No Hit
CTTCCTTGCTGCACTTAACGCCCAGACCAATGTGGCCATTGTTGTCGCCGATGGCAACGAAGGCCTTGAAACGGG12.941176470588235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAACAAACAGACCGAGCGCGAGATCAAAGATAAA12.941176470588235No Hit
TCTCCCCATGCAGATCGGAAGAGCACACGTCTCCCCATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACA12.941176470588235Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TAACTAAACATTAAATACATAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGATAAATAAAAA12.941176470588235No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers