FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136
Sequences flagged as poor quality0
Sequence length75
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1913.970588235294118No Hit
AAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACAACCAAAGAAAAAAAATAAAA10.7352941176470588No Hit
GATAAGTAGAGGACTCGTTCCGGGAGATGCCCTGCATTATTTAACCATTATCAAAGTCATACATCTGTTTCTGTC10.7352941176470588No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGCTTTTATT10.7352941176470588No Hit
ACGAAATGGACGTTGACCTCGCTTGAGGGCAAGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGC10.7352941176470588No Hit
AGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGT10.7352941176470588No Hit
TAACTGTGGTAATTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTA10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAATATCATCGACTACATCAGCGT10.7352941176470588No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTATTGATTTATAGAAGCAGTTT10.7352941176470588No Hit
GACAAACATACTATATATTTTGCCATCTCTCAAATAACATTGCATGAGACGATGGCGCAAATAGCCACAATTAGT10.7352941176470588No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG10.7352941176470588TruSeq Adapter, Index 12 (96% over 26bp)
GATACAAGTATTTAATCACATATAAGAACTCCACCGGTAATACGCTTACATACATCCCACATCCTGTCTCTTATA10.7352941176470588No Hit
TAAAACGATCATTCGATCGCTTGGTATTAGCCAAAAAAAAGCACATGTCCCATAAGGTTAATCAATTAAATGCAT10.7352941176470588No Hit
GTGCTCTTCCGATCTCGTGGGGGTGCTCTTCCGAAAAAAAAAAAAAACTTTCTCTTATACAAAGATCCGTGGCAA10.7352941176470588No Hit
ACCTTGAAAGGCCAGTGCTTCATGTCCTCTGCGATCTAGGGGTCGTCGTATTTTCGGCCGATAAGTCGATTGGCG10.7352941176470588No Hit
ATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAACTTTAACTGTCTC10.7352941176470588No Hit
AAAAAAAACAAAAACAAATAAAAATAAAAAAGTAATAAAATTAATCTTAAACAAAAAAAAAAAAAAAAAAAACAA10.7352941176470588No Hit
GTCCTGGGATTCTGGTTGATCCTGCCAGTAGTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACC10.7352941176470588No Hit
GAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTACCTGTCTCTTATACACA10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAGATCGGAAGAGCCCCGGGGTGTTATACCCAGAGCGGAGCCCACGAGAAAAGG10.7352941176470588No Hit
CGCGAATATTGAGCTTACAAATCAGCCGGCCAATAGACAAAATGGCTGTCTCTTATACACATCTCCGAGCCCACG10.7352941176470588No Hit
CTTTTAATTCGTGTGTACTTAGACATGCATGGCTTAATCTTTGAGACAAGCATATAACTACTGGCAGGATCAACC10.7352941176470588No Hit
GACAGAGGACGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG10.7352941176470588Illumina PCR Primer Index 5 (96% over 28bp)
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCCTGTCTCTTATACACATCTCCGAGCCCACAGACTACGCT10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAA10.7352941176470588No Hit
AGTTTCAGCTTTGCAACCATACTTCCCCCGGAGCCCAAAAGCTTTGGTTTCCCGGGAAGCGACTGAGAGAGCCAT10.7352941176470588No Hit
GCCAGCGGCTCCCTTGGCGCCAGCACCACCCTTGGTGACCTTGCCGGCACCTGTCTCTTATACACATCTCCGAGC10.7352941176470588No Hit
GATCGGAAGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGG10.7352941176470588RNA PCR Primer, Index 12 (96% over 29bp)
CTCCCGCTCTCCTCTCGCTCTCTGCTGCTAAACTCGACCTCTCCTCTATCACCTATTTGTCTGTATCTCTTCCAA10.7352941176470588No Hit
ATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAAGGTTCATGTTTTAATTGCA10.7352941176470588No Hit
GTTAAAACGTTCGTAGTTGAACTTGTGCTTCATACGGGTAGTACAACTTACAATTGTGGTTAGTACTATACCTTT10.7352941176470588No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACCTGTCTCTT10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAACTCTAATACACAGCGCCGAGAAAACGAGACAAGCGCAC10.7352941176470588No Hit
TACCTATTGTCAATGGTCAAATGAATCCCAATAATCAGCAATAAGAAGTTTTTTCATTTTGATGAATACTGTCTG10.7352941176470588No Hit
ATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGCTGTCTCTTATACACAT10.7352941176470588No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGCACCTGTCTCTTATACACATC10.7352941176470588No Hit
AGTGAATGGGCAGCGCCTTGCGCGGCACGTGCAGCGCCAATTGAGGCACCGATACAGTGAACACTCGCTCGCGCA10.7352941176470588No Hit
CCTGTTTTCCGGTCCTCGAGGAACGGGGAAAACCAGTTCCGACAAAATTCTGGCGAAGGCGATTAATTGCGAGAA10.7352941176470588No Hit
CCTTCTTGCGCTGCACCAACAGCTTGGCGTAGTCCGCGGAAGCCTCCTTGGAAGCGAGCTGCCGCTTCTTCTTCA10.7352941176470588No Hit
GTACACACGAATTAAAAGTGAAAACGCAAAAGGCTCATTATATCAGTTATGGTTCATTAGATCGTTAACAGTTAC10.7352941176470588No Hit
CTGGGGGACGACGCAGCAACAGGGACATCTGACGGTAGGCCACAGCCTGCTTGGACAAATCATCGTAGATGATCA10.7352941176470588No Hit
TTCTTGATCTGTGCAATTTTGTCCCAAAAATGTTACTTCATTCATGAAAAATGAACATTTTTATAAAGCTCGCTT10.7352941176470588No Hit
ATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATG10.7352941176470588No Hit
GCCTGAAGAAGGGCGAGCTGGGATGCGCCTCCATTTGCAATGGCGGCGGTGGAGCCTCCTCCATTCTCATCGAGA10.7352941176470588No Hit
TTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATAACCTGTCTCTTATACACAT10.7352941176470588No Hit
CTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCT10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGATAAAAAACAAAAGAAAAATAACAGAAAAAAT10.7352941176470588No Hit
CCATAAGGTTCATGTTTTAATTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAACTGTTAACGATCT10.7352941176470588No Hit
CGAGAGTGCTGTTCTCTATCCAAGGGTCGGGCACTTAGCTCTCCTGAACTAGAATCAATGCTGAACTTATTGCCC10.7352941176470588No Hit
TGTCTGCCCTATCAACTTTTGATGGTAGTCCTAGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCG10.7352941176470588No Hit
AAGAAGGGCAGGGGGGCGGGTCTTCCCGCCCCTCGGCTTCGTTTGGAGGAAGGCATGGGAAGGGTTGTCGGGTAT10.7352941176470588No Hit
TTATCATCCTGGGCATCGTTGGTGGCCTGCTCCTCCTTGCGCTGGTCACCTATGTGCTGTGGAAGGTAGGCTTCT10.7352941176470588No Hit
TTCCTGTACTCGGTGTTACTGCAAGGGGGGACGGAGAAGGCTAGGCTAGCCGGGCGACGGTTGTCCCGGTTTAAG10.7352941176470588No Hit
AGTATGGGAAGAGCACACCCCGGGGGAGATCGGAAGCTGTCTCTTATACACATCTCCGAGCCAACGAGACTAGCG10.7352941176470588No Hit
ATTTCAGGGTGATAGACCGTTAAGAAGAAAAGAGAACTCTGCCCGGTGCACACGCCGTCGTCACCGCGTTCAGTT10.7352941176470588No Hit
CGTTCGCCTTGCATGACTATGTATTCATCATGCAATACCTAGCTTATGCTAAGTGGGTTTCCCCATTCAGAAATC10.7352941176470588No Hit
ATTTAGTAATACATATTCCAAAATAAGATTAAATAAGTACACCCTTAAAGGCTATTGTGTATGATTGAAGATTAC10.7352941176470588No Hit
AATATATTCTTTTTCTGTTTACATCGAGAAGCTGAATGTTTTTTTTTTATCACAATGCTCAAAAAATGGGGAATT10.7352941176470588No Hit
CCCATAAGGTTCATGTTTTAATTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAACTGTTAACGATC10.7352941176470588No Hit
CTTGTATCTTTTCATATGTTACTCCTATTTAAAAACCTGCATTAGTGATCTTAAACGAGTGTTATTGTGGTCCGG10.7352941176470588No Hit
ATTGTGGCGCGCCACCAGCATCCTTGCCAACCACCCCTATCATCGCGAAGGGTGAGGGCATGGCGGGAAAGCGTC10.7352941176470588No Hit
CTAACATACATAAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTAC10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAATTTCTGAGTCTGCAGCCGGATGGTAGACACATGCGATAGAACAACGAA10.7352941176470588No Hit
GGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTAGACCG10.7352941176470588No Hit
GGGCCGGGAACGTATTCACCGCAGCATTGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGC10.7352941176470588No Hit
AAAAAAAACGAAACTAATACGAAAAAAAAAAAAAAAAAACAGACAAATATAAAAAAATAAACGCACACTCTAAAA10.7352941176470588No Hit
GTCCCAAGGCCAGCTCAGTGCGGACAGAAACCACACATAGAGCAAAAGGGCAAATGCTGACTTGATCTCGGTGTT10.7352941176470588No Hit
GGCCTCTAGGGCCTGTTGCAATTCCAATTTCTGCTCTTCCAGCAAACGAATTGTTTCCAGCTGCAGTGGATTATT10.7352941176470588No Hit
GATACAAGTATTTAATCACATATAAGAACTCAACCGGTAATACGCTTACATACATAAAGTTATAGTACTAACCAC10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAA10.7352941176470588No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGCAGGGACACCCCACCCCGGGGGAAGTGTACCGCATGGCG10.7352941176470588No Hit
CTTTCAATTAGCACATAATGTATTTTTCTGAATAAATAACTTTGTTAAGAATTGTTATATGTCTGTCTCTTATAC10.7352941176470588No Hit
ACCTTAGGACCGTTATAGTTACGGACGCCGTTTACCGGGGATTCGATCAAGAGCTTCGCGTTAGATAACCCCATC10.7352941176470588No Hit
AGCTACAAGGAGGTGCTCGCAGTCAAATGGGGGCCTGTGAACTAGTGAGAGGAACTTAGAGAACTCTAAGACCTA10.7352941176470588No Hit
TCCGATCTTCAACGGGATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAATCCTTTTAACAAGGACCAATTG10.7352941176470588No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTGCTCCTTGCAAAAAAAAGGGGCGGGG10.7352941176470588TruSeq Adapter, Index 12 (95% over 21bp)
CGTCTCCCGCATGAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA10.7352941176470588Illumina PCR Primer Index 10 (96% over 29bp)
TTATACTCATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG10.7352941176470588Illumina PCR Primer Index 10 (96% over 28bp)
AAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATATAAGCCTGTCTCTTATAC10.7352941176470588No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAACAAAAAAAA10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAT10.7352941176470588No Hit
CACCCGGGAGACGTGTGCTCTTCCGATCTCGTGTGCACTACAGAGCTGGCCTGCGATACGTGTGCTCGGCCGGTG10.7352941176470588Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCAAGACGATATCCTTTTTGTGGGAAAATGTGTACTGCAGCACTTTCTGGTGTGTTAAGTGGGCATAAAACGC10.7352941176470588No Hit
CAAAATAGGCAATCAATTCCAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAACGGAAAATCCGGGTTGAGAAGAA10.7352941176470588No Hit
GTCCCATAAGGTTCATGTTTTAATTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGCTGTCTCTTATAC10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAATAAAATAAAACTAATATGTACAAAGTAATAGTAAATGACAACAAAAACAC10.7352941176470588No Hit
GTGTAGCTACTTTTATGGCTCTCTCAGTCGCTTCCCGGAAAACCAAAGCTTTTGGGCTCCGGGGGAAGTATGGTT10.7352941176470588No Hit
GCCCTGGGAGACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTG10.7352941176470588TruSeq Adapter, Index 7 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAACAAAAAAAA10.7352941176470588No Hit
CTTAAAAGTGAAAAAAAACAACAAACACGCCTAAGAAAGAACAAACCGCGATAACAACCATGGACTCACAAAAAA10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACACCCAAAAAAAAAAAAAAAAGACCCCACCAGCCAAG10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAA10.7352941176470588No Hit
CTTCACCAATGTATATGTGAAGAACTTCACTGAGGAATTCGACGATGAAAAACTGAAGGAATTCTTTGAGCCCTA10.7352941176470588No Hit
ATATTAAAGTTGTTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTTCATACGGGTAGTACAACTTACAATTGTG10.7352941176470588No Hit
CCTGCCGAGGCGAGCGATGCTAAGCACGCAAGTCGCACGGCGCCTTCGGGTGATGTGTCCATGAGTCTGCGTCCA10.7352941176470588No Hit
CTTCAAGGCTGAATTGAACGAGTTCCTGACTCGCGAACTCGCCGAGGATGGCTACTCCGGCGTGGAGGTCCGTGT10.7352941176470588No Hit
AGCATTGGAAGATCTCGAAAGGACAAGTGGTCACGACCATCCCGATGTAGCAACCATGCTGAACATTTTGGCTCT10.7352941176470588No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAGAACAACCACAAGTCTCCCATATATATTAAACA10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAATAAAAGAG10.7352941176470588No Hit
TCGTTAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAC10.7352941176470588No Hit
ATCTAAGGAACCATAACTGATATAATGAGCCTTTTGCGGTTTCACTTTTAATTCGTGTGTACTTAGACATGCATG10.7352941176470588No Hit
GCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTCTGTCTCTTATACACATCTCCGAGACCA10.7352941176470588No Hit
GCTCCAATCTGATCGATATCATTGCCAAGAACGGATTCGAGAAGTTCAAGCAGTTCTGCTACGCCATCGAGTGCG10.7352941176470588No Hit
ATGCCGGACAGATCGAAGGTGCCCAATGCATTGTTGTCCTTCGTCATCGCACGTTCGCCCTCATACACCTGAATT10.7352941176470588No Hit
ATCCTAGTCACACAGCTCACACAGCTACGTACAACACAACACGAATCCAGCATTCCATCATCGCGCCGCCTCCGG10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA10.7352941176470588No Hit
GCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATACTGTCTCTTATACAC10.7352941176470588No Hit
GTATGCTGACGCCCAGCAGTCCTTGGCCGCCCCAGTTGTTGCTCGGTGTAAGGGTCAGTTCGCGGACCGTCTGCG10.7352941176470588No Hit
TTTTTAAATAGGAGGAACATATGAAAAGATACAAGTATTTAATCACATATAAGACTGTCTCTTATACACATCTCC10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAGCAACGCACCCAGAAAGATATAAAG10.7352941176470588No Hit
CTTCCGATCTAAGCAGGGATTCTGGTTGATCCTGCCAGTAGTTATATGCTAGTCTCAAAGATTAAGCAATGCATG10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAGAAATAATAT10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAA10.7352941176470588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAACAAAAAAAAA10.7352941176470588No Hit
CGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACAT10.7352941176470588No Hit
GCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGTTCTTATTTCATTATCCCATGC10.7352941176470588No Hit
ATGTGTACTACAATGAGGCGTCCGGTGGCAAGTACGTGCCCCGCGCTGTCCTTGTCGATCTGGAGCCCGGCACCA10.7352941176470588No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers