FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5432441
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG153140.2818990579004908No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG139980.25767422048394084No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA133300.24537772246398995No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA125810.23159018201946419No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAA113800.2094822566871872No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCAGTTT113610.209132505995003No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTT104160.19173701104162935No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG95120.17509624126612694TruSeq Adapter, Index 7 (96% over 28bp)
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTTCATTATCCCATGCACAG90880.1672912784510683No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATT87970.16193457048129928No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATG77400.14247738723715547No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC74520.1371759030608892No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTTTCGCTTAA73970.1361634668466717No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT71840.13224257750797477No Hit
TCTTATATGTGATTAAATACTTGTATCTTTTCATATGTTCCTCCTATTTAAAAACCTGCATTAGTGCTCTTAAAC70320.12944457197050094No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69710.12832168816927786No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA66220.12189732019178856No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC65870.12125304260092286No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA64140.1180684705089296No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC62690.11539932048962888No Hit
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTC62110.1143316604819086No Hit
TTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTT59810.11009783631336263No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAAC59500.1095271904471673No Hit
GCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTACCGGCCCACAATAACACTCGTTTAAGAGCACTAATGCAGG55500.10216401798013085No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA16850.031.5352521
GCCGTCT32050.029.06720446
GTATGCC32900.028.83635742
CCGTCTT33650.027.89018247
TATGCCG35100.026.93066243
CTTGAAA35300.026.87580957
CGTATGC35550.026.78385441
TTCGGAA20300.026.1724412
CTCGTAT35150.026.00902739
TGCTTGA37000.025.73421555
TGCCGTC37150.025.7269645
AAAGGGG34850.025.4435668
GCTTGAA37100.025.38588556
CTGCTTG37100.025.29452554
ATGCCGT38000.024.96621144
CGTCTTC38350.024.3821248
GTCTTCT39500.023.49755949
AAGGGGG37800.023.3666169
GGTCCAA68050.022.20493711
CTTCTGC41300.022.13927551
Produced by FastQC (version 0.11.2)