FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63
Sequences flagged as poor quality0
Sequence length75
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23.1746031746031744No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAA11.5873015873015872No Hit
ACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGATCTTGCCGGAACGATGATCGTCGAAGATCTCGAAAT11.5873015873015872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAGCAAAAAAAAAAATTAAT11.5873015873015872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAGAC11.5873015873015872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA11.5873015873015872No Hit
GTATCTAGGACTACCATGGTTGCAACGGGTAACGGGAAATCAGGCTCCCTCTCCGGGATCGCACGCTGCTTCCGC11.5873015873015872No Hit
ATTTTCGTTTTGGTGTTAGTCGTTTTGCTCAGGCTGGTGCTCAGGGTGCAGATCTTGTAGACAGTGTTCGCTGCG11.5873015873015872No Hit
GAACAAGAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAACCACCCAACAAGAAAAAAAAAAA11.5873015873015872No Hit
TCTTTCAAATGTCTGCACTATCAACTTTTGATGGTATTATATAGTACTACAATGTTTGCAAAGGGTAACGGGGAA11.5873015873015872No Hit
ATTAAGGAATTTGAATACTTAATTAAACACTAAAATTTGTTAAAAGAAAAAGAAAAGGCCCTTTCTCTAATAAAA11.5873015873015872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAGGCGACCACATCGAAGTAAGCCAGCACCCGCCAAAATAAACCACTACA11.5873015873015872No Hit
TTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATAAC11.5873015873015872No Hit
CCATCGGAGGACAAGGTGGAGGCCCCCTTCTCAGCCTCATACCCGGGTGGCAGAGCATAGCGGCGGACACAGTGC11.5873015873015872No Hit
AAAAAAAAAAAAATATTCAAAATAAACAAAAAACAAGAGCAAAAAATAGAAACCGAAAAAAGCTATTAAAATCAT11.5873015873015872No Hit
CATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTAATATTTGTTGAACTC11.5873015873015872No Hit
ATACATAACATGCACATCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAATAAAAAAAAAAAT11.5873015873015872No Hit
ATAAAAAATATGTTGCTGTTAAAACAATCGTAGTAGACCTAGAACTTCAAACGTCAAATACAAGTTAAAATTGTA11.5873015873015872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAACAAGAAAAAAAAAAAAAA11.5873015873015872No Hit
ATATAAGTGGGCGTGATCTTTTACGCCGTTGTGCTGCATAAATTAAAAAGACGCGTGCTCATTCTAAGTATGTCA11.5873015873015872No Hit
GGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGTAAACGGC11.5873015873015872No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.5873015873015872No Hit
ACAGAGGTCTTATTTCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGT11.5873015873015872No Hit
AAAGAGCACAATAATAAAAAAAAAAACAAAACAAAAAAAAAAAAAAACAACACAAAAAAAAGAAGCCACCCTAAG11.5873015873015872No Hit
CTCAAATATATTAAATAGATATCTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTTCATTCATGTTGGCAGTAA11.5873015873015872No Hit
CAACAACACTATCACAACAATTCGTCCATCTTAAACTATTTAAAATGAACAACTTCAAATTCACGCTCAACCCCT11.5873015873015872No Hit
AAAAAACGGTCCTAATGAAAAAAAAAAAAAAAAAAAAAAAAATAAAAACAGCACGAAAGGCGTAACGATTTTTGA11.5873015873015872No Hit
GTTGGTTTGTCCCATTATAAGGACACTAGCTTCTTAAATGGACAAAATGCGTCTAGCAATAATGAGATTGAGAAA11.5873015873015872No Hit
GGTTCAACTTTGGAGATTTCAATGAGAAAGACTGTTCTGGACCTGGCCGCAATAGACGCCTCCTATGAAGAGTTC11.5873015873015872No Hit
AAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTC11.5873015873015872No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAAACTTACCAGGTCCGCACATAAGTGTGTAAGAC11.5873015873015872No Hit
AAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGG11.5873015873015872No Hit
GGACTACCATGGTTACAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA11.5873015873015872No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACGAAGATATTCTAAAGCATTGGCCTAAGAGGTT11.5873015873015872No Hit
GGTAATGATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAG11.5873015873015872No Hit
AATAGAAGCAGTTTGGGGGCATTAGTTTTACGACGCGAGAGGTGAATTTCTTGGACCGTCGTAAGACTAATGCCC11.5873015873015872No Hit
ATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTACCGGCCCACAATAACACTCGT11.5873015873015872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAA11.5873015873015872No Hit
GATCTGAAGAGAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAGTACCGAGCGGTTAAAAATCCAGCAC11.5873015873015872No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATAACTGACTTGTAGCCATCTACGCGCC11.5873015873015872No Hit
ATCTAAGGAACCATAACTGATATAATGAGCCTTTTGCGGTTTCACTTTTAATTCGTGTGTACTTAGACATGCATG11.5873015873015872No Hit
GACGTAAGACTAACTTAAGCGAAAGCAGATGCCAAACATGAATTAATTAAACAAGAACGAAAGTTAGATTTTATA11.5873015873015872No Hit
GCAGCAGGGTGGTGATCTGGGCCAGATCTACAAGCGTCTGGTGACCGCCGTCAACGAAATCGAGAAGCGTGTGCC11.5873015873015872No Hit
GGATATGAGTCCTGTATTGGTATTTTTCGTCACTACCTCACAGCGCTGGGAGTTGGGAATTTACTCGCCTGCTGC11.5873015873015872No Hit
GCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTACCGGCCCACAAT11.5873015873015872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAACAAATGGAGAACCCCCCAAGTACATAAACAAAAAAAAAACCAAACAGAA11.5873015873015872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAA11.5873015873015872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAGAACAAGAAAACAATGTAGGAAGGAAATGG11.5873015873015872No Hit
TAATATACGCTATTGGAGCTGGAATTACAGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGGTCCTTGTTAAAA11.5873015873015872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAGC11.5873015873015872No Hit
GATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTT11.5873015873015872No Hit
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTC11.5873015873015872No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA11.5873015873015872No Hit
AGCTCTCATAGTTTATATAGTTTATAAGCATTTTCTATAGTCTACGAAGAAAATTCGTCACTGGTGTGTGTGTGT11.5873015873015872No Hit
ATCAACTTTTGATGGTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGCGGAATCAGGGTTCGATTCCGGAG11.5873015873015872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAGA11.5873015873015872No Hit
CGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACAT11.5873015873015872No Hit
GTTCAAAGTAATAGTACCGGCCCACAATAACACTCGTTTAAGCTGACTATTAAACAAATGTAAGAAGCACCGAGA11.5873015873015872No Hit
GAACCATAACGGATATAATGAGCCTTTTGCGCTGTCACTTATAATTCGCTGTATCTAAAACACATCTCCGAGCCC11.5873015873015872No Hit
CGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGGGATTGTGAACCTGTCTCTTATACACAACTCCGAG11.5873015873015872No Hit
CCATGATATATAGAGTTCAACCAAAATAACGATCTTTCGAACGCTTTATTTTAGCCTAATAAAACCAGATGTCCC11.5873015873015872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAACAAGAACGAAATTT11.5873015873015872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGA50.069.025
TGATGGT50.069.029
CTTTCAA50.069.02
CTACAAT50.069.047
CAATGTT50.069.050
TGCACTA50.069.014
TTTTGAT50.069.026
GTTTGCA50.069.054
TTGCAAA50.069.056
TGGTATT50.069.032
TATATAG50.069.038
CAACTTT50.069.022
TGTCTGC50.069.010
TTCAAAT50.069.04
TGCAAAG50.069.057
CGGGGAA50.069.069
GTACTAC50.069.044
GGGTAAC50.069.063
TAACGGG50.069.066
TGTTTGC50.069.053