FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68
Sequences flagged as poor quality0
Sequence length75
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57.352941176470589No Hit
GGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAAATGTAAGTTGTACTACCCGTATGAAGCACAAGT11.4705882352941175No Hit
AAACCAATAGCAATCGCCAATTCTCCCCATCATCGTGGACAAGTGCTTAACCAAAGCATCAACTTGGTTGACAGT11.4705882352941175No Hit
AACCTGCAACGTATCGCAGATGTGCGTTAATTACGCACAGACACTTAGAACCCTATAACGGAAAAATCGACTAAA11.4705882352941175No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATATGAAAACCAATGAAAGCAGAACAG11.4705882352941175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAGTAAGTGTTGAAAAATA11.4705882352941175No Hit
CATATGCGGCGTGATGAAGACGTACAGACAGAAGAGTCGATCCAGCCAACAGGGCGTGGCCGAGCCCGCAACTGT11.4705882352941175No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACAATACTATA11.4705882352941175No Hit
AACAAGAGCAAATACAAACAAAAAAAAAATCAACATTATTGCACATCAGAAAAAAAAAAAAAAAAAAAAAAAAAA11.4705882352941175No Hit
CCCGTATGAAGCACAAGTTCAACAACCAACGTTTTAACAGCAACCTGTCTCTTAAACACATCTCCGAGACCACCA11.4705882352941175No Hit
CTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCATGGTAGTCCTAGATACTACCATCAAAAGTTGAT11.4705882352941175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCAACCAACCATGGAAATAC11.4705882352941175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAACAAAAAAAGAAGAAGATAAAGAGAA11.4705882352941175No Hit
CACGACAAAAACCCAAACTACATCGCTTCATTTGAATCAAGAAAAACACCACAAACATCACCAAGCACTACCCCC11.4705882352941175No Hit
AAAAACGACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCAAAAACCCTCACCGGAATATAACAAAAAAAA11.4705882352941175No Hit
AAAACCTGCTGAACACATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACAAACATGAGATATAAGAAA11.4705882352941175No Hit
TCTGTGCATGGGATAATGAAATAAGACCTCTGTTATGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATCTGTA11.4705882352941175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAACA11.4705882352941175No Hit
CTAAAAATCCAATAACAAGCACCAATTGCACGCCAAGACTAGTTACATCAACCGCTCTAATTACAGCTCCAATAT11.4705882352941175No Hit
AAACAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAGAAACCCCGTAAAAAGTTAAAAAAAAAAAAAAA11.4705882352941175No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATCCCCGTAGCTGTCTCTTATACACATCTCCGAGCCCACG11.4705882352941175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACCGGCCAAACCACGCAGAGCGGACAGCGCGCCGGGACCG11.4705882352941175No Hit
CAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGAAAACCACACGCCTGGCCAGAAAAAAAGAGAGTG11.4705882352941175No Hit
GTTGTGTAAATTGCTGTCAGAACAGTTTTGATCCACGAGGATCGAGGATCAGGCTCCTGTGTATTGCATTTTTCT11.4705882352941175No Hit
ATATGTGTAAACAAGACGAAAAAACAAAACAAAAAATGCAAAATGCGAGTTTGTGAAATATGCAAATTAATGAAA11.4705882352941175No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAATACAATCGATAAAGAAAAACATACGACGGACC11.4705882352941175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCACACCGCACGAGGAAATAAAACAAAAAAAGAAGACAA11.4705882352941175No Hit
ATAAAAAAATACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCGCTAAAAAAATTTACAAAA11.4705882352941175No Hit
ATAAAAACAAGTACAACCAAAAGAACACGAACTTCAACCACACACGAAAGATCCGCAACGGTAATAAACACCACA11.4705882352941175No Hit
ACCAAGGCAACCACTACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCAACCCCAACAAAAAAAAAAAAA11.4705882352941175No Hit
AACCAGGAGTCCATCCATGCCTCGCGTCAAACGTGGTGTAACCGCACGCGCCCGCCACTGTCTCTTATACACATC11.4705882352941175No Hit
GTCCAGCAGGGCATCGCGTTGGGCCTCCTTCTCCTCGCGGGTGCAGATCGTGATATTCAGACCCATCACAATCAT11.4705882352941175No Hit
TATAAAATACATCCGATAATTCTGCATAATTTGCTTGCTTTTGTGTGTTTTGTTTTTGTTGTTTGTTAAGCATTT11.4705882352941175No Hit
GCCAATATCATACTGAATGAGCAAAAACTGGAAGTATTCCCTTAGAAAACCAGTACAAGACAAGGATGCCCTCTC11.4705882352941175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAGTGCTTATAGGCTAAAAACAAACGAACGCAAGAGCCATA11.4705882352941175No Hit
GTGGAGCCGTACCTGTTGGTTTGTCCCATTATAAGGACCCTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATA11.4705882352941175No Hit
TCGTGATATTGTTTGAACTCTAGATATCATGCAGATCGTATGGTCTTGTGCCGACGACAGATCTTTCAAATGTCT11.4705882352941175No Hit
AACTTCATCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTGATATTTTAAAAATGTACAAATACTTAGAA11.4705882352941175No Hit
TTGACATCGTAGCCCGAACATAGGACGCACAGCGGGCGAGACTGCCGAGACGTGGTGTCACATAGAGTTGTCTCC11.4705882352941175No Hit
ACAATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCAACAAGACCGTGCAAAAGAAACAAGAC11.4705882352941175No Hit
TAAAAATAACTGATATAATGATACTTTTGATTTTTCAATTTTAATTATTGTGTACTTAGACTGTATATTATACAA11.4705882352941175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAATAAAATTTAAAAAGAATTTAAATAAAAATAAAAATTTTAAAAATATTTAA11.4705882352941175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAGAAACAAAAAAACAAAAACAAA11.4705882352941175No Hit
AATCTGTCAAGATCTCTTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACAAAGATCCGAAAAAAAAAAA11.4705882352941175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAACACCAAATAAAAAAACAAAAGTAA11.4705882352941175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAGCAACGAAAGAATGGTTTTAAGAAAAA11.4705882352941175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACAAAAAGGTATATATAAAAAAAAAACACAA11.4705882352941175No Hit
AAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAACACAAAAACAAAAAAAAAAAA11.4705882352941175No Hit
ATCCTGGCCCTGGCCTACATCATCATACTCTTCAACATCATCAGCATTATCCGACGCAATCGGGGCTCAAATTAA11.4705882352941175No Hit
TCGGAAGAGCACACGTCTCAGAATGGAAGAGATCCCGAGACACGACGCAACCGCACATGAGAAGCAAGCGAAGGG11.4705882352941175No Hit
ACACATAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACCAAATTAAATTAATCAAAAAAAAAGTCCAAGAG11.4705882352941175No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGC11.4705882352941175No Hit
GTTAATAATATAGTAATAGCTCCTGATAGTACTGGCTTTCTCTTATACACATATCCGAGCCCACGAGCCCGGAGC11.4705882352941175No Hit
CGTGTGCTCTTCCGATCTGACATGGGAGACTTTTGCTATTCCGATATGACATGGGAGAGTATTTCTGTTACGAGT11.4705882352941175No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA11.4705882352941175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAACAACGAAAGCTTGCACATAAACACAAAAGCGACACCAACGACGGCGAGC11.4705882352941175No Hit
CTGTTAACGATCTAAGGAACCATAACTGATATAATGAGCCTTTTGCGGTTTCAGTTTTAATTCGTGTGTACTTAG11.4705882352941175No Hit
GCTTACACCTCTCGCCTATCGAAGTTCTGTTCAAAAACCTCGTCCGAGAACTTGTATAGAGAAGGATTTCCCGCG11.4705882352941175No Hit
CCTGTATTGTTATTTTTCTTCACAACCTCCCCGAGCTGGGAGTTGGTAATTTACTCGCATGATGACTTCCTTAGA11.4705882352941175No Hit
GAATTTCACCTCTAGCGTCGTAATAATAATGCCCACAAACTGCTTATATTAATCCTGTGTATTATACAAATCACC11.4705882352941175No Hit
ATAATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGTAATTGATAACACCACACACACACACACACACAAA11.4705882352941175No Hit
GACTGTAACAAGGGTTTCACACTCGCGGTCACGGCTAAAGTTAAACTTAGTTAAAGAATTCATCGTACAGTAGCC11.4705882352941175No Hit
CTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGACTTCAAATGCCCTGTCTATTATACACATCTCAGAGCCCA11.4705882352941175No Hit
AATATTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCAAACAAATAATCGTAGAATGAACAGAGAA11.4705882352941175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCTC50.069.035
GAGAAGG50.069.059
GAGAACT50.069.046
CCTATCG50.069.015
TTGTATA50.069.052
ACTTGTA50.069.050
AGAAGGA50.069.060
AGGATTT50.069.063
CGCCTAT50.069.013
GCCTATC50.069.014
TTTCCCG50.069.067
TTCAAAA50.069.030
CCTCGTC50.069.038
GAAGTTC50.069.021
GTTCTGT50.069.024
GTCCGAG50.069.042
TCGAAGT50.069.019
CTCTCGC50.069.09
TTCTGTT50.069.025
GGATTTC50.069.064