FastQCFastQC Report
Mon 14 May 2018
H27VGAFXY_n01_zt14wtlarvs38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH27VGAFXY_n01_zt14wtlarvs38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86.451612903225806No Hit
AACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAATACCTCGCCGCTAGGGTATAAACGATGCACTAGC10.8064516129032258No Hit
ACATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAAAGTTT10.8064516129032258No Hit
TGTAAGAGCACTACTGAAGGTTTTTAAATATTAGGAACATATGAAAAAAGACAAGTATTTAATAACATATAACAA10.8064516129032258No Hit
GTGTACTCATTCCAATTACAGGGCCTCTGATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCACGAGCTG10.8064516129032258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGTAAAACAAAAGAAAAGAAGA10.8064516129032258No Hit
CAATTGGGTGTAGCTACTTTTATGGCTCTCTCAGTCGCTTCCCGGGAAACCAAAGCTTTTGGGCTCCGGGGGAAG10.8064516129032258No Hit
CCTGAGAAACGGCTACCACATCTAAGGAAGGCAGCAGGCGCGTAAATTACACACTCCCAGCTCGGGGAGGTAGTG10.8064516129032258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGAA10.8064516129032258No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAATCCTTTAACAAGGACCAATTGGAGG10.8064516129032258No Hit
GCATGGGATAATGAAATAAGTCCTCTGGTCTGCTTTCATTGGTTTTCAGATGAAGAGCTAATGATTAATAGAAAC10.8064516129032258No Hit
GCTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGATGGCACCAGACTTGCCCTCCCATTGGTCCTGGTT10.8064516129032258No Hit
CTCCTACTTGCTGGCCACGGCCTTCTTGGGCTTGGCCTGGGTGCCCTCGCGGAATCCGTTGCGGAAACGGCGGCG10.8064516129032258No Hit
GTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAACTTTAATATACGCTATTGGA10.8064516129032258No Hit
ATTGGAATGAGTACACTTTAAATCCTTTAACAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGC10.8064516129032258No Hit
CCAGTGGACGAAGGCACGCTTGGCGTACATCAGATCGAACTTGTGGTCCAGACGGGCCCAGGCCTCGGCGATGGC10.8064516129032258No Hit
AGCTTTGGTTTCCCGGGAAGCGACTGAGAGAGCCATAAAAGTAGCTACACCCAATTGCTAGCTGGCATCGTTTAT10.8064516129032258No Hit
GGAGACGTGTGCTCTTCCGATCTCGAGAGGGCTCTTCCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACT10.8064516129032258Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCATACGGGTAGTACAACTTACAATTGTGGTTAGTACTATACCTTTATGTATGTAAGCGCTGTCTCTTATACACA10.8064516129032258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGAACAGACCCAGAAACACATCACAGAGAAAAA10.8064516129032258No Hit
GACGTACGGGGGTGTGATGCCTGCCCGGTGCTGGAAGGTTAATTGATGGGGTTAGCGTAAGCGAAGCTCTTGATC10.8064516129032258No Hit
ATAAATACCTATTATTTCTATCCTAGCAATTGTGTGGCACAGTAGATTACTGTATCTTATACACAGCTACGATCC10.8064516129032258No Hit
ACCTTAGGACCGTTATAGTTACGGCCGCCGTTTACTGGGGCTTCGATTCGTAGCTTCGCAGAAGCTAAACTCCTC10.8064516129032258No Hit
CCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA10.8064516129032258No Hit
GTGATCACGCCATTTGGCTCGATCCTCACCGATCGATGGAACTTGTAAGTGGTCGAAGGACCTTTCTCGTTGATT10.8064516129032258No Hit
GTCCTAGATACTACCATCAAAGGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC10.8064516129032258No Hit
GTATCACCGATTTTTTGATATCTCTCCTTGAGTGTATCAGCCTTTAGGAATTGCCACGGCAGTGAAACCCTTAAG10.8064516129032258No Hit
GGGCTATGTTGCCCTCGACTTTGAGCAGGAGATGGCCCCCGCTGCCAGCAGCTCCTCGTTGGAGAAGTCCTACGA10.8064516129032258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAA10.8064516129032258No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCTAAGGCTGGCAGTTATAGACAACTCGGCCCCCA10.8064516129032258No Hit
CGGCTACCACATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCGGCTCGGGGAGGTAGTGACGAAAAAT10.8064516129032258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8064516129032258No Hit
AAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTACCGAC10.8064516129032258No Hit
AAACAAAAAAATAAACACATATAACAAAAACACCTCTAATAATAATACATACATAAAGGTCACACAATTATACTC10.8064516129032258No Hit
ACTTTGCAAGTACTCGGCTGACACCGCGCTGTCCAGTGGACACCGCACTGCCCATGGATATAGGACCTCTCTCGG10.8064516129032258No Hit
TATAAGGCAATTGCGTTCGCTTTTTTTACGCCTAATAAGTTCAAAGTAATACTACGGTCCATGATTAAAAAGAGT10.8064516129032258No Hit
GCTAAGATCCGTAAGGCCGCTCGTGAGCTGCTGACCCTCGACGAGAAGGACGAGAAGCGTCTGTTCCAGGGTAAT10.8064516129032258No Hit
ATATTGGGTTGAAGACAAAAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAGAGGAAAGTTAGAAGTTTA10.8064516129032258No Hit
GCCCTCCAATTGGTCCTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGGCGCCGATGCGGGGCCGTGT10.8064516129032258No Hit
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGCTGTCTCTTATA10.8064516129032258No Hit
GATCGGAAGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCAGCTTGAAAAAAAAAAGTGGGGGGGGGG10.8064516129032258Illumina PCR Primer Index 12 (96% over 26bp)
AAAAAAATTACAAAAAATATAAATAACTTTAAATACCTTTGTAATAACAAAATAAAAGCAAAATAAAAAAAACAA10.8064516129032258No Hit
CTCAGTCGCTTCCCGGGAAACCAAAGCTTTTGGGCTCCGGGGGAAGTATGGCTGTCTCTTATACACATCTCCGAG10.8064516129032258No Hit
CATGAAGTTCTGCCGCTGACCGGATCCCTTGTTGCCCGCTCCGTGCTTATCCTGTCTCTTATACACATCTCCGAG10.8064516129032258No Hit
TAACACATTTATCCAATTAACTTATAAATCTATAATTAAACATAAATCATAAAAATGTCTAGTATAAAAAGCAAA10.8064516129032258No Hit
CTGCAGCACTAAGGGGCAGAAACCCCCTAACACTTAGCACTCATCGTTTACGGCGTCGACTACAGTGTATCTAAT10.8064516129032258No Hit
GATGAACAGATTGTCGACAGGTCGCTGAGCAAGCGCCATCGTTTGGGATCGGCTGCTTTCTCTTATACACATCTC10.8064516129032258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAACACACGAAAAAAAACAAAACAGCACAC10.8064516129032258No Hit
GGCCTCCACGTCCTTGATGTCGATCTTCTCGCAGGGCGTTGTGAAGTCAGCGGTTCTGGGGCGTCCATATCCATA10.8064516129032258No Hit
GGTCTGAAGATTGAGGACTTCTTGGAGCGTCGTCTGCAGACGCAGGTGTTCAAGCTGGGACTTGCCAAGTCCATC10.8064516129032258No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTT10.8064516129032258No Hit
TCGCCGAGCTGGGAGTGGGTATTTTACGGGCCTGCAGCCTTGGTTAGATGTGGTAGCCGATTCTCAGGCTCGCTC10.8064516129032258No Hit
GTATTTAATCACATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACCTGTCTCTTATACA10.8064516129032258No Hit
GTACAAAGGGCAGGGACGTAATCAACGCATGCTGATGACATGCGCTTACTAGGCATTCTCGTTGAAGAGCAATAA10.8064516129032258No Hit
GTTCGCTGGGCGTGAAGTGCGACACATCCCTGCAGACCTGGAAGCCATCCGGTCCGATTTTCGCCACGGCTCCCG10.8064516129032258No Hit
GCGGGGGGAACAGGCGGCACCATCTTGTTAATGTCGCCGTAGCGAGTGGGAGCTGTCTCTTCTACACATCTCCGC10.8064516129032258No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATCACTAGATTAGTGTCAGCTAAACACCAAACCGAGCCAC10.8064516129032258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACTAAAGTTTAACACCACAACTCAACAAAGAGTC10.8064516129032258No Hit
TCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAATTAAAACATGAACCT10.8064516129032258No Hit
GCAATTGGGTGTAGCTACTTTTATGGCTCTCTCAGTCGCTTCCCGGGAAACCAAAGCTTTTGGGCTCCGGGGGAA10.8064516129032258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAACACATATAATAGAAAAAGAGACTAA10.8064516129032258No Hit
AAAAAATCTAGGACAACAATGGTTCCAACAAAAAAAAAAAAAAAAAAAAAACAATCCGCAGAGCAACACTGAGAA10.8064516129032258No Hit
AGAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAACCAAAACAAATAAAAAAAAA10.8064516129032258No Hit
AAAAATTAAATAAAAAAAAATTAATAAAATAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAT10.8064516129032258No Hit
ATTTTGAACCGCATGGTTCAAAAGTGAAAGACGGTCTTGCTGTCACTTATAGATGGATCCTGTCTCTTATACACA10.8064516129032258No Hit
GATCCACTTCGGCGCGTCGTCGAATTCCGGCGGATGGTGATGGCCCTGCACCGCTTCGTTCTGCGCGTGGGCATG10.8064516129032258No Hit
GTGCATGCCCTAGTGCGAACGCAGGGACCCATAACCACCGATGCAGATCGTCGAGCTACGCTGGAACGACGAGTC10.8064516129032258No Hit
GGAGTGCACTCTGCACGTGGACGGCTGCCTGCCCATCTACAACAAGGGATCCTGTTGCCCAGTGCGCTACAGTTG10.8064516129032258No Hit
TCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCT10.8064516129032258No Hit
TTCCAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGAGGTA10.8064516129032258No Hit
TGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCTAAGG10.8064516129032258No Hit
ATCTAGGACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATCTG10.8064516129032258No Hit
GCATTAGTGCTCTTAAACGAGTGTTATTGTGGGCCGGTACTATTACCTGTCTCTTATACACATCTCCGAGCCCAC10.8064516129032258No Hit
AAGGAGGGCAAGGAGGGTGGCGAGCAGGAGAAATCAAACGAACAGGAGGGCACTGAGGGCGACAAGATCGAGAAG10.8064516129032258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAGAAGAAACAAAGAATAAA10.8064516129032258No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCATCTGCTTGAAAAAAAAAACGGGGGGGCCGGGCG10.8064516129032258Illumina PCR Primer Index 12 (96% over 26bp)
GCAATGGACACCGTATCCGTGGACAGGTATCCGGACAAGCTGCCCGAGCCATACTGGATCTGTCTCTTATACACA10.8064516129032258No Hit
CAGTTATCCAAGTAACTGTTAACGATCTAAGGAACCATAACTGATATAATGAGCCTTTTGCGGTCTGTCTCTTAT10.8064516129032258No Hit
CAACTATACATTAATTTATATAATCAAATAAACTGTTGACTACTAATAATTGATAAAATACAACTAAAATTAAAT10.8064516129032258No Hit
GTTATATGCATGTCTCAAAGATTAAGCCATGCATGTATAAGTACACACGAATTAAAATTGAAACAGCAAAAGTCT10.8064516129032258No Hit
AGGTAATGATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGTACCGTCGTAA10.8064516129032258No Hit
CATAAAGGTATAGTACTAACAACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTAT10.8064516129032258No Hit
TTTTTTGGGGGAAACTCGAATTAATACCGCATACGACCCGCGGGAGATCGGAAGCTGTCTCTTATACACATCTCC10.8064516129032258No Hit
GTCTTGCTGTCACTTATAGATGCATCCGCTCTGCATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCAACGAT10.8064516129032258No Hit
GTATAGGGGCTGACGCCTGCCCGGTGCTGGAAGGGTAAGAGGAGTGGTTAGCTTCTGCGAAGCTACGAATCGAAG10.8064516129032258No Hit
GTCTTAAGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA10.8064516129032258No Hit
ATACATATATCACCAAGCCTTTTATCATCCTAAAAAAAAAAAAAAAAAACAATATTAAACAAAACACATTGCAAC10.8064516129032258No Hit
CCACGTAGCTATGCGTAAGGTAGCCATTATTTATTTGATTGTTGGGCTACTGTCTCTTATACACATCTCCGAGCC10.8064516129032258No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG10.8064516129032258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGATAATAAAAAAAAAACTCTGTACAGCATGT10.8064516129032258No Hit
TACTAAGACTAAAAAAATATAAATCAAAACAAAAAAAAAAATAAAATAAACAAAAAAAAAAAAAAAAAAAATAAA10.8064516129032258No Hit
CCGTCGGATGAGACAGTGGACACTACCTCGTTGGGGTCAAAGCCCTTGGGCAGGGTATACTTGCGCACAAAGTGA10.8064516129032258No Hit
TATAGTGAGTTGGCACATGATCTGGCTCCATTCGTGACCCACATCCTAATCCTGTCTCTTATACACATCTCCGAG10.8064516129032258No Hit
AAGTAAGACACCAATCAAGAAAAAAAACAAAAACAAAACTTTTAACAGAAACTAATATAAAATACTCATATCCAA10.8064516129032258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGACAACA10.8064516129032258No Hit
CTACCTCCCCGAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCT10.8064516129032258No Hit
GAAGAGCACACGTCTCCCGTTTAAGAGCGGAAGAGATCACCAGTGCCGTTGAAGCGAGGCTGCGCTGCATAGAGC10.8064516129032258No Hit
GCACCCCGGCGTAGATCGGAAGAGCCCACGAGACCGGAGCCTATCTCGTATTCCGTCTTCTTCTTGAAAAAAAAA10.8064516129032258RNA PCR Primer, Index 27 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA10.8064516129032258No Hit
GAACAGAGGTCTTATTTCATTATCCCATGCACAGAATATTCACTGTATCTTATACACATCTCCGATCACACGAGA10.8064516129032258No Hit
ACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAA10.8064516129032258No Hit
ACTTTTGATGGTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGAAATCAGGGTTCGATTCCGGAGAGGT10.8064516129032258No Hit
AATAAACACACATGTGCTATTAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAACACAAGATA10.8064516129032258No Hit
GCACCTGAGGCTGTGCCACGCGAGCGCATGCAGCAGCCGAACGCAACGGAGTGGGCACCGCTCCGGCTGTGCCCG10.8064516129032258No Hit
CTCTACTACTTTAGCATAAATAAAATGCTTTGTACCCAGCCCTATGCTTCTATCTAGATTACAGAAAGATGAAAA10.8064516129032258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAGGAATA10.8064516129032258No Hit
CTCTTAATGACAAGGCCTCAGGAACGGCCCACCCGAAGGGTGACACACCCCTATCCAAAACCCACACACCTCCGC10.8064516129032258No Hit
GTATAGGGGCTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGAGTGGTTAGCTTCTGCGAAGCTACGAATCGAAG10.8064516129032258No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATCTGTCTCTTATACACAT10.8064516129032258No Hit
AGTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGC10.8064516129032258No Hit
AACAAGACGAGCAAAACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACACACTGACACTTAAACACAACTA10.8064516129032258No Hit
ATACTATATAGTAAGGCCCTATATAGCTAGGCGCTAAGCTCTCATATATAAGCATTCAGACATCAGACATTCAAA10.8064516129032258No Hit
GTATGGTCTTGTACCGACGACAGATCTTTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACTAC10.8064516129032258No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAGCGATCGCAAGATCGTTATATTGGTTGA10.8064516129032258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCACACCCCAAACCGAAAAAAAAAAAAAAA10.8064516129032258No Hit
GAGGTAGGGTCCAGCGGCTGGAAGCGCACCGCGGTTCGGGGGGTGTCCGGTGCGCCCCCGGCGGCGCTTGAAAAG10.8064516129032258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAATAAAAATAAAAG10.8064516129032258No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers